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Preparation method of anti-infection calcium sulfate/drug-loaded silk fibroin microspheres/mineralized silk fibroin nanofiber bone repair material

A nanofiber, calcium sulfate technology, used in pharmaceutical formulations, microcapsules, drug delivery, etc., can solve problems such as increasing patient pain and prolonging treatment time, achieving good biocompatibility, promoting adhesion, and good self-curing properties. Effect

Active Publication Date: 2019-06-25
SHANXI JINBO BIO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This treatment method not only increases the pain of the patient, but also prolongs the treatment time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Preparation of α-type calcium sulfate hemihydrate: fully mix 300 g calcium sulfate dihydrate and 1.5 g sodium oleate, add 1200 mL of distilled water and stir evenly, then place in a closed high-pressure reactor, heat to 120 ° C, and the pressure is 0.138 MPa, Keep the pressure and heat at constant temperature for 7 h. Then the prepared material was taken out and placed in an electric ventilation oven at 120 °C to dry for 4 h, cooled naturally to room temperature, ground with a mortar, and sieved with a 180-mesh stopper to obtain α-type calcium sulfate hemihydrate powder with uniform particle size.

[0015] Preparation of drug-loaded silk fibroin microspheres: add vancomycin to silk fibroin solution at a mass ratio of 1:20, and stir magnetically for 30 min at room temperature to obtain a concentration of 20 mg / mL~ 60mg / mL drug-loaded silk fibroin solution. In the experiment, the concentrations of drug-loaded silk fibroin solutions were 20 mg / mL, 40 mg / mL and 60 mg / mL, r...

Embodiment 2

[0021] The preparation of α-type calcium sulfate hemihydrate, drug-loaded silk fibroin microspheres, and mineralized silk fibroin nanofiber solutions is the same as in Example 1.

[0022] Mix 0.1g calcium sulfate dihydrate, 0.2g drug-loaded silk fibroin microspheres, 0.1g vancomycin, and 1.6g α-type calcium sulfate hemihydrate evenly. Add 1mL~1.4mL mineralized nano-silk fibroin fiber solution with a concentration of 3wt%~15wt% to prepare the anti-infective calcium sulfate / drug-loaded silk fibroin microspheres / mineralized silk fibroin nanofiber bone repair material. The measured final setting time of the material is 92min~130min, the compressive strength is 4.53MPa~6.35MPa, and the compressive fracture work is 14.59J~18.83J. As the concentration of silk fibroin increased from 20mg / mL to 60mg / mL, the drug release rate of the drug-loaded silk fibroin microspheres gradually decreased. The drug loading rate and encapsulation rate were 4.46%~4.64%, 87.98%~92.08%, and the diameter o...

Embodiment 3

[0024] The preparation of α-type calcium sulfate hemihydrate, drug-loaded silk fibroin microspheres, and mineralized silk fibroin nanofiber solutions is the same as in Example 1.

[0025] Mix 0.2g calcium sulfate dihydrate, 0.2g drug-loaded silk fibroin microspheres, 0.1g vancomycin, and 1.5g α-type calcium sulfate hemihydrate evenly. Add 1mL~1.4mL mineralized nano-silk fibroin fiber solution with a concentration of 3wt%~15wt% to prepare the anti-infective calcium sulfate / drug-loaded silk fibroin microspheres / mineralized silk fibroin nanofiber bone repair material. The measured final setting time of the material is 36min~50min, the compressive strength is 4.25MPa~5.99MPa, and the compression fracture work is 15.38J~19.41J. As the concentration of silk fibroin increased from 20mg / mL to 60mg / mL, the drug release rate of the drug-loaded silk fibroin microspheres gradually decreased. The drug loading rate and encapsulation rate were 4.54%~4.72%, 87.29%~91.39%, and the diameter of...

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Abstract

The invention provides a preparation method of an anti-infection calcium sulfate / drug-loaded fibroin microsphere / mineralized fibroin nanofiber bone repair material and belongs to the field of biomedical materials. The preparation method comprises steps as follows: 5wt%-10wt% of calcium sulfate dihydrate, 5wt%-10wt% of vancomycin-loaded fibroin microspheres, 5wt% of vancomycin and 75wt%-85wt% of alpha type calcium sulfate hemihydrate are taken as raw materials and mixed uniformly, then a mineralized fibroin nanofiber solution with the concentration being 3wt%-15wt% is added, and the anti-infection calcium sulfate / drug-loaded fibroin microsphere / mineralized fibroin nanofiber bone repair material is obtained. Osteogenesis is promoted while infection at the part with bone defects is effectively controlled. After the surface and the interior of the bone material are absorbed, a remodeling process of bone tissue is simulated with deposition of new bones, and the material can be integrated into metabolism of intravital bones and is finally replaced with own bone tissue.

Description

technical field [0001] The invention belongs to the field of medical biological materials, and in particular relates to a preparation method of an anti-infection calcium sulfate / drug-loaded silk fibroin microsphere / mineralized silk fibroin nanofiber bone repair material. Background technique [0002] Infected bone defect is difficult to treat due to the dual lesions of infection and bone defect, and the course of treatment is long, and it is prone to muscle atrophy and local scars, which seriously affect limb function. So far, infected bone defect is still a difficult and urgent treatment problem in clinical practice. After infection, bacteria form a biofilm on the surface of the implant, which makes the simple bone grafting fail because the infection is not easy to control. Therefore, patients often cannot perform primary bone grafting, but need to thoroughly debride the wound. After the infection factors are eliminated, the wound heals, and the blood vessels After reconst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/42A61L27/54C04B11/02
CPCA61L27/42A61L27/54A61L2300/252A61L2300/406A61L2300/602A61L2300/622A61L2400/12A61L2430/02C04B11/02
Inventor 连小洁刘世超刘力明吴亚丽黄棣魏延
Owner SHANXI JINBO BIO PHARMA CO LTD
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