Method for preparing alkyl glycoside

Abstract

The invention discloses a method for preparing alkyl glycoside, and belongs to the technical field of fine chemical engineering. The method comprises the steps that bacillus is firstly cultured, bacillus strains are obtained, then a certain number of bacillus strains are moved for ultraviolet mutagenesis, the strains with high reductase activity of acyl coenzymes A obtained after mutagenesis and high fatty alcohol productivity are selected for culture, and then fermentation is carried out; after fermentation is finished, glucose is added into the fermentation broth, and reacting is carried out to generate alkyl glycoside under the action of aldolase. According to the method, the aldolase is adopted as a catalyst, dehydration condensation between the glucose and dehydration of the glucose are avoided, the reaction time is shortened by 12 h through the efficient catalyzing performance of enzymes, the conversion rate and the yield are increased, the yield is increased by 95% or above, and the purity of the alkyl glycoside reaches 96%. The method is easy and convenient to operate, no organic reagent is adopted, environment friendliness is achieved, pollution is avoided, and industrial production is promoted.

Description

technical field [0001] The invention discloses a method for preparing alkyl glycosides, which belongs to the technical field of fine chemicals. Background technique [0002] Alkyl glycosides, referred to as APG, have excellent ecological and toxicological properties, excellent physical and chemical properties, and good compatibility. It has low surface tension, strong detergency, rich and fine foam, and can be compounded with any type of surfactant It has obvious synergistic effect, and is non-toxic, harmless, non-irritating, and its biodegradability is better than any other type of surfactants. functional surfactant. [0003] Alkyl glycosides can be used as raw materials for daily chemicals such as shampoo, shower gel, facial cleanser, laundry detergent, hand sanitizer, dishwashing liquid, and vegetable and fruit cleaning agents; they are also used in soap powder, phosphorus-free detergent, and phosphorus-free laundry powder and other synthetic detergents; it can also be ...

AI Technical Summary

Problems solved by technology

[0007] The technical problem mainly solved by the present invention: In the process of producing alkyl glycosides by the traditional method at present, the reaction speed of the condensation reaction between glucose and fatty alcohol is still slow, the reaction time is long, and what is used in the condensation reaction is an acid catalyst, so it is easy to occur The dehydration condensation between glucose and the dehydration of glucose itself make the alkyl glycoside products have high impurity content, dark color, and difficulty in removing impurities. A method for preparing alkyl glycosides is provided. First, by culturing Bacillus, Obtain Bacillus strains, then pipette certain Bacillus strains for ultraviolet mutagenesis, select strains with high acyl-CoA reductase activity after mutagenesis and thus high fatty alcohol production for cultivation, and then carry out fermentation, fermentation At the end, glucose is added to the fermentation broth, and under the action of aldolase, the reaction generates alkyl glycoside. The present invention uses aldolase as a catalyst to avoid the dehydration condensation between glucose and the dehydration of glucose itself, and utilize the high efficiency of enzyme Catalytic, the reaction is shortened by 12 hours, and the conversion rate and yield are improved, and the yield is increased to more than 95%, and the purity of the alkyl glycoside reaches 96%. The invention is easy to operate, does not use organic reagents, and is green and pollution-free , conducive to industrial production