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Huidouba polysaccharide and preparation method thereof

A technology of Glycyrrhizae polysaccharides and polysaccharides, which is applied in the field of Glycyrrhizae polysaccharides and its preparation, can solve the problems such as the absence of Glycyrrhizae polysaccharides, achieve the effects of improving glucose tolerance, facilitating industrial production, and improving glucose and lipid metabolism

Inactive Publication Date: 2016-08-31
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, the effect of Glycodida polysaccharides in treating diabetes has gradually attracted the attention of diabetes workers and health food researchers. reports on impact

Method used

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  • Huidouba polysaccharide and preparation method thereof
  • Huidouba polysaccharide and preparation method thereof
  • Huidouba polysaccharide and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: the preparation of polysaccharide

[0036] Weigh 200g of the dry raw material of Douba huidouba, add 6L deionized water, extract at 95°C for 3h, filter through 400 mesh gauze, repeat the extraction twice under the same operating conditions, combine the extracts, and centrifuge at 4000rmp / min for 10min to obtain Supernatant: Add 200g of activated carbon to the supernatant, shake at 55°C for 2.5h, and centrifuge at a speed of 8000rmp / min for 15min to obtain the supernatant, which is light yellow; add 1250mL of Sevag reagent to the supernatant, and stir magnetically for 15min. Stand still in the separatory funnel for 15-20min, remove the middle layer and the lower layer, keep the upper layer, repeat the operation 8 times, add absolute ethanol to make the final ethanol volume fraction 80-90%, and stand at 0-4°C for 12- 24 hours; centrifuge, collect the precipitate, and freeze-dry to obtain the solid powder of Douba polysaccharide.

Embodiment 2

[0037] Embodiment 2: the preparation of polysaccharide

[0038] Weigh 200g of the dry raw material of Douba huidouba, add 6L deionized water, extract at 95°C for 3h, filter through 400 mesh gauze, repeat the extraction twice under the same operating conditions, combine the extracts, and centrifuge at 4000rmp / min for 10min to obtain Supernatant: Add 200g of activated carbon to the supernatant, shake at 55°C for 2.5h, and centrifuge at a speed of 8000rmp / min for 15min to obtain the supernatant, which is light yellow; add 1250mL of Sevag reagent to the supernatant, and stir magnetically for 15min. Let it stand in the separatory funnel for 15-20 minutes, remove the middle and lower layers, keep the upper layer, and repeat the operation 8 times. The supernatant was purified and graded with a 10kDa ultrafiltration membrane, and the solutions flowing out from the retentate end and the permeate end were collected to obtain polysaccharide 1 and polysaccharide 2 respectively; absolute e...

Embodiment 3

[0039] Embodiment 3: Molecular weight determination of polysaccharide

[0040] The molecular weight of polysaccharides was determined by high performance gel permeation chromatography (GPC). The chromatographic conditions used are: TSK-GEL G-5000PWXL column (7.8mm×300mm) chromatographic column connected in series with TSK-GEL G-3000PWXLcolumn (7.8mm×300mm) chromatographic column; 0.02mol / L KH at pH6.0 2 PO 4 The solution was used as the mobile phase, and the flow rate was 0.6mL / min; the column temperature was 35°C; the detector was a waters 2414 differential detector; Dextran series dextran was used as the standard. The GPC gel permeation chromatograms of polysaccharide 1 and polysaccharide 2 are as follows: figure 1 and figure 2 shown. The average molecular weights of polysaccharide 1 and polysaccharide 2 were 36571Da and 6277Da respectively.

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Abstract

The invention belongs to the fields of health foods and medicines and discloses a Huidouba polysaccharide and a preparation method thereof. The preparation method comprises the steps of extracting Huidouba as a raw material with hot water, carrying out ethanol precipitation to obtain rough Huidouba polysaccharide, carrying out activated carbon decoloration, removing protein by virtue of a Sevage reagent, carrying out separation and purification by virtue of a ultrafiltration technique, and screening to obtain a Huidouba polysaccharide 2 with relatively low molecular weight, wherein the peak molecular weight Mp is 6277Da, the weight-average molecular weight Mw is 9703Da, and the number-average molecular weight Mn is 7144Da. By virtue of the preparation method, the Huidouba polysaccharide 2 can be efficiently prepared through separation, is capable of remarkably reducing blood glucose of type 2 diabetes KKAy mice, glycosylating hemoglobin, improving glucose tolerance and glucolipid metabolism, has an in-vitro antioxidation effect and is applicable to the preparation of health Huidouba polysaccharide foods and medicines with the hypoglycemic effect.

Description

technical field [0001] The invention belongs to the field of health foods and medicines, and relates to a polysaccharide from Glycyrrhizae polysaccharide with hypoglycemic effect and a preparation method thereof. Background technique [0002] Diabetes is a metabolic disease caused by multiple etiologies that seriously threatens human health. It is characterized by chronic hyperglycemia, accompanied by disorders of carbohydrate, protein, and fat metabolism caused by insulin secretion and / or action defects, resulting in systemic multiple Chronic organ damage, dysfunction, or even failure. According to statistics, 10% of the world's population suffers from diabetes, and the International Diabetes Federation predicts that if no measures are taken, 642 million people worldwide will suffer from diabetes by 2040. China is the largest country with diabetes. At present, there are more than 100 million adult patients over the age of 20, and the incidence rate is as high as 11.6%. Th...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P3/10
CPCA61K31/715A61K2236/331A61K2236/51A61K2236/53C08B37/00C08B37/0003
Inventor 李琳周新李冰黄吉东张霞苏健裕徐振波
Owner SOUTH CHINA UNIV OF TECH
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