Preparation method of skin microscopic tissue slice of hynobiidae animals

Abstract

The invention discloses a preparation method of a skin microscopic tissue slice of hynobiidae animals and belongs to the technical field of microscopic tissue slices. The preparation method includes following steps: 1) dehydration: successively soaking skin tissue, which has been subjected to fixing treatment, of the hynobiidae animals into ethanol solutions being 50%, 70%, 80%, 90%, 95% and 100% in concentration gradient to dehydrate the skin tissue, wherein dehydration time in each concentration gradient is 45-60 min; (2) transparent treatment: soaking the dehydrated skin tissue into a mixed liquid of 100% ethanol and dimethylbenzene for 2-3 min, and then soaking the dehydrated skin tissue into dimethylbenzene for 10-30 min; (3) wax soaking: soaking the skin tissue being subjected to the transparent treatment into a mixed liquid of dimenthylbenzene and paraffin for 20-30 min, and then soaking the skin tissue into paraffin respectively by two times, each time lasts for 45-60 min; and (4) embedding the wax-soaked skin tissue, and performing slice cutting, slice expansion, slice pasting, slice baking, dyeing and seal-fixation to obtain the skin microscopic tissue slice. The preparation method is simple in process and convenient to operate. The prepared slice is complete and shows a clear image structure.

Description

Technical field [0001] The invention relates to a preparation method of amphibian skin microscopic tissue slices, in particular to a preparation method of small salamander skin tissue slices for microscopic observation, and belongs to the technical field of microscopic tissue slices. Background technique [0002] The skin is the first natural barrier of the amphibian organism. In the process of adaptation and evolution, it plays the role of breathing, regulating water balance, ion transport, resisting predators, resisting bacteria, and fungi. These functions reflect The complexity of skin morphology characteristics and the potential physiological characteristics of skin structure play an important role in maintaining the survival of amphibians, developing and adapting to vast habitats and diverse ecological environments. [0003] Salamanders are endemic amphibians in Asia. They are also the third largest family among the 10 families of Urgodae. 66 species are known worldwide, belon...

AI Technical Summary

Problems solved by technology

However, this method has the following deficiencies: (1) incomplete dehydration, resulting in incomplete paraffin penetration, resulting in broken tissue or cavities during sectioning, disintegration of sections during spreading, poor support and coloration during staining, etc.; ( 2) The dehydration effect of n-butanol is not good; (3) Due to the lack of a transitional step in the wax immersion process, the xylene carried from the transparent step enters the paraffin, resulting in a decrease in the paraffin concentration. In addition, the wax immersion time is too long to cause the tissue block to be brittle and hard

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