Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting gourmet powder through concentration isoelectric point technology

An isoelectric point, monosodium glutamate technology, applied in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of environment, water source hazards, low fermentation efficiency, and difficulty in waste liquid treatment, etc. The effect of promoting emission standards, reducing costs, and increasing industrial added value

Inactive Publication Date: 2015-11-11
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the following problems exist in the production process of sodium glutamate: On the one hand, the extraction of monosodium glutamate usually adopts the isoelectric-isoelectric method, and glutamic acid is crystallized by adding concentrated sulfuric acid to adjust the isoelectric point, while the sulfuric acid produced in the production process Ammonium waste liquid has brought great difficulties to waste liquid treatment and caused direct harm to the environment and water sources
On the other hand: low fermentation efficiency, high cost of raw materials, and declining corporate profits
In short, the defects in the preparation process of monosodium glutamate are mainly the low fermentation efficiency, the large amount of waste water produced, and the high concentration. Overcoming the above defects is the direction of our research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for extracting monosodium glutamate by using concentrated isoelectric point technology comprises the following steps: 1) preparing Corynebacterium glutamicum seed liquid, 2) preparing Brevibacterium lactose fermenting seed liquid, 3) preparing mixed seed liquid, 4) fermenting, 5) Concentration and isoelectricity, 6) preparation of monosodium glutamate, 7) preparation of biological agents, 8) biological treatment.

[0030] Specifically, the method includes the steps of:

[0031] 1) Preparation of Corynebacterium glutamicum seed solution:

[0032] Corynebacterium glutamicum (Corynebacterium glutamicum) ATCC13032 is inserted in Corynebacterium glutamicum seed culture medium, inoculum size is 5%, at 30 ℃, 180rpm shaking table is cultivated 18h, the Corynebacterium glutamicum seed liquid that obtains; The components of Corynebacterium acidic acid seed culture medium are (below all are mass percent): glucose 3.2%, corn steep liquor 2.8%, K 2 HPO 4 0.2%, Na 2 HP...

Embodiment 2

[0055] 1. Strain fermentation comparison test (same fermentation conditions): Corynebacterium glutamicum ATCC13032 was used as the fermentation strain, and the maximum glutamic acid production can reach 200g / L (see the applicant's previous patent cn201210211393 ); and adopt the mixed strain of the present invention to ferment, the output of glutamic acid reaches 226g / L, has improved 13%, is better than single strain fermentation greatly.

[0056] 2. Examples of wastewater treatment effects

[0057] According to the process of Example 1, the waste water extracted by fermentation of sodium glutamate is processed, and the data of COD, ammonia nitrogen, and total nitrogen are measured by sampling; compared with Example 1, the control group 1 does not add liquid bacterial agent; the control group 2 is compared with Example 1 The adsorbent carrier is wheat bran only. Sampling and determination of COD and ammonia nitrogen data, the test data are shown in Table 1:

[0058] Table 1 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle size (mesh)aaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of gourmet powder extracting technology in the biological fermentation technology and discloses a method for extracting gourmet powder through the concentration isoelectric point technology. The method comprises the following steps that firstly, a corynebacterium glutamicum seed solution is prepared; secondly, a brevibacterium lactofermentum seed solution is prepared; thirdly, a mixed seed solution is prepared; fourthly, fermentation is carried out; fifthly, concentration isoelectric operation is carried out; sixthly, gourmet powder is prepared; seventhly, a biological agent is prepared; eighthly, biological processing is carried out. The method overcomes the technological defects that the fermentation efficiency of the gourmet powder is not high, and the energy conservation and emission reduction cannot meet the standard.

Description

technical field [0001] The invention relates to the technical field of monosodium glutamate extraction in the biological fermentation industry, and specifically provides a method for extracting monosodium glutamate by using a concentrated isoelectric point technology. Background technique [0002] Monosodium glutamate, the scientific name is sodium glutamate, and the chemical name is monosodium α-aminoglutaric acid. It is a salt formed by sodium ions and glutamate ions. Among them, glutamic acid is an amino acid and sodium is a metal element. . The main ingredient of seasoning MSG commonly used in life is sodium glutamate. Monosodium glutamate is a commonly used flavoring agent in daily life, which can increase the umami taste of food and help improve the digestibility of food by the human body. In addition, sodium glutamate has very important functions and is widely used in food, medicine, industry and agriculture. [0003] At present, there are the following problem...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P13/14C07C229/24C07C227/40C02F3/34C12R1/01C12R1/15
Inventor 赵凤良孙钦波刘化庄翟德亮董力青
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com