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Cadmium-IgG chelate as well as preparation method and application thereof

A chelate, chelation reaction technology, applied in the field of detection, to achieve the effect of improving accuracy and repeatability

Active Publication Date: 2015-10-21
上海保亿仁生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For the serious problem of cadmium pollution, the object of the present invention is to provide a kind of cadmium-IgG chelate and preparation method thereof, and establish the qualitative and quantitative detection method of cadmium-IgG chelate, so that quantitative detection cadmium-IgG chelate Application in evaluating the degree of cadmium pollution in an area

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  • Cadmium-IgG chelate as well as preparation method and application thereof
  • Cadmium-IgG chelate as well as preparation method and application thereof
  • Cadmium-IgG chelate as well as preparation method and application thereof

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preparation example Construction

[0061] The present invention also provides a kind of preparation method of cadmium-IgG chelate, comprises the following steps:

[0062] A) chelation reaction of cadmium and IgG: adding cadmium ions to human IgG or IgG recombined according to biological methods for chelation reaction to obtain a reaction solution;

[0063] B) Extraction of purified cadmium-IgG chelates: Immunoaffinity chromatography is used to remove unreacted IgG and excess cadmium ions in the reaction solution to obtain cadmium-IgG chelates. The specific steps are as follows:

[0064] (1) Dissolving sample: add physiological saline to the reaction solution obtained through step A) to redissolve the cadmium-IgG chelate

[0065] (2) Equilibrium chromatography column: Use the dilution buffer to flush the pipeline of the chromatography column, fill the chromatography column with a filler that can specifically bind to IgG, and continue to use the dilution buffer to balance the chromatography column after loading t...

Embodiment 1

[0159] Example 1 : the preparation method of cadmium-IgG chelate, comprises the following steps:

[0160] A) chelation reaction of cadmium and IgG: adding cadmium ions to human IgG for chelation reaction to obtain a reaction solution;

[0161] Reagent preparation:

[0162] 1) Borate buffer (0.01M): Weigh 0.31g of boric acid and dissolve it in 400ml of ultrapure water, adjust the pH to 9.0 with 0.1M NaOH, and dilute to 500mL.

[0163] 2) IgG solution: Weigh 4.0mg IgG and dissolve it in 4.0mL 0.01M pH9.0 borate buffer solution, fully shake and dissolve, and prepare a 1.0mg / mL protein solution;

[0164] 3) 5mmol / L EDTA+200mmol / LNaHCO 3 Solution: weigh EDTA·2H 2 O 1.86g, NaHCO 3 Dissolve 16.8g in 900mL ultrapure water, adjust the pH to 8.0 with 1.0M NaOH and dilute to 1000ml, autoclave, and store at room temperature;

[0165] 4) ITCBE (purchased from Japan Tongren Chemical Research Institute, item number M030)

[0166] 5) Dialysis bag (molecular weight cut off 14000) (Bio...

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Abstract

The invention discloses a cadmium-IgG chelate as well as a preparation method and an application thereof. The cadmium-IgG chelate is formed by chelating cadmium ions and IgG by virtue of sulfydryl or / and cysteine residues. The invention establishes a quantitative and qualitative detection method of the cadmium-IgG chelate, so as to perform a quantitative detection on the application of the cadmium-IgG chelate in evaluating the cadmium pollution level in an area. By performing the quantitative detection on the cadmium-IgG chelate in sera of a group of people in the area, the condition of cadmium-polluted people in the area can be indirectly reflected, so as to indirectly reflect the cadmium pollution level of the area. The cadmium-IgG chelate quantitative detection method established by the invention has the advantages that the accuracy is greatly improved, and the repeatability of the detection is greatly improved.

Description

technical field [0001] The invention relates to the field of detection, and more specifically relates to a cadmium-IgG chelate and a preparation method and application thereof. Background technique [0002] IgG is mainly synthesized and secreted by plasma cells in the spleen and lymph nodes, and exists in the form of monomers. In the process of individual development, the body synthesizes IgG later than IgM, and begins to synthesize it in the third month after birth, and it is close to the adult level at 3 to 5 years old. IgG is the main antibody component in serum, accounting for about 75% of total Ig in serum. IgG is the only Ig that can cross the placenta and plays an important role in natural passive immunity. In addition, IgG also has the functions of opsonizing phagocytosis, ADCC and binding SPA. Due to the above characteristics of IgG, IgG plays a major role in the immune protection of the body, and most antibacterial, antiviral, and antitoxin antibodies belong to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/00G01N33/68G01N33/96
CPCC07K16/00G01N33/6854G01N33/96
Inventor 张积仁阳帆董欣敏吴婧蔡睿孙遥赵乙木
Owner 上海保亿仁生物医药有限公司
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