Protein-free, hydrolysate-free and serum-free culture medium and preparation method thereof
A serum-free medium and hydrolysate technology, applied in the field of serum-free medium without protein and hydrolysate and its preparation field, can solve the problems such as difficulty in controlling the differences between product batches, interference of research results, and difficulty in clarifying components, and achieve Small difference between product batches, simple ingredients, and the effect of reducing production costs
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[0024] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. Unless otherwise indicated or defined, all terms used have their ordinary meanings in the art, which will be understood by those skilled in the art.
[0025] The preparation method of protein-free, hydrolyzate-free serum-free medium is as follows:
[0026] Add Tween 80 into a clean container with a capacity of 1000 ml, add 50 ml of water for injection to dissolve, add linoleic acid, linolenic acid, and arachidonic acid in sequence, wait until it is fully dissolved, rehydrate to 500 ml with water for injection, and then Add sodium chloride, glucose, and fructose in sequence, stir until clear, rehydrate with water for injection to 900 ml, and then add in sequence: calcium chloride, magnesium sulfate, potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, L fine hydrochloride Amino acid, L aspartic acid, L asparagine, L cyst...
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