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Antitumor drug carrier and application method thereof

An anti-tumor drug and carrier technology, applied in the field of biomedicine, can solve the problems of severe systemic toxicity and low drug concentration, and achieve the effects of convenient storage, good biocompatibility and good stability

Active Publication Date: 2015-06-03
GUANGDONG JINAN ESTABLISHED SELENIUM SOURCE NANO TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an anti-tumor drug carrier and its application method to solve the problems of low local drug concentration and severe systemic toxic reaction during the chemotherapy of tumors.

Method used

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  • Antitumor drug carrier and application method thereof
  • Antitumor drug carrier and application method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0066] The above-mentioned phycobiliprotein is made into phycobiliprotein nanoparticles of the antitumor drug carrier of the present invention, and the preparation method comprises the following steps:

[0067] S1, prepare phycobiliprotein aqueous solution;

[0068] S2. Use a pH regulator to adjust the pH of the phycobiliprotein aqueous solution away from the isoelectric point of the phycobiliprotein;

[0069] S3. Desolventizing phycobiliproteins from the aqueous phycobiliprotein solution to form nanoparticles;

[0070] S4. Using a cross-linking agent to cross-link the precipitated phycobiliprotein nanoparticles to obtain an aqueous solution of tightly cross-linked phycobiliprotein nanoparticles.

[0071] Wherein step S1 is specifically, using the dry protein powder of the above-mentioned phycobiliprotein, dissolving it in water, and the concentration of the phycobiliprotein aqueous solution is 2-200 mg / mL.

[0072] Wherein step S2 is specifically, adjusting the pH of the abov...

Embodiment 1

[0089] Embodiment 1 Preparation of Phycobiliprotein Nanoparticles

[0090] Under normal temperature and pressure (15-35°C, 1 standard atmospheric pressure), prepare a water-soluble phycoerythrin solution with a mass concentration of 2 mg / mL, and adjust the pH of the phycoerythrin solution to 7.0 with 0.1N aqueous sodium hydroxide solution. Slowly add 8 mL of absolute ethanol dropwise under continuous stirring at a rate of 0.5 mL / min to desolventize the phycoerythrin to form a nano-protein sol. After the dropwise addition, 23.5 μL of 8% (volume fraction) glutaraldehyde was added, mixed evenly under a magnetic stirrer, and the reaction was continued for 1 hour. After the reaction, the phycoerythrin nanoparticles were centrifuged for 2 rounds (the centrifugation speed was 5000rpm, and each round lasted 10 minutes), and then resuspended with 8 mL of double distilled water to obtain a colloidal suspension of phycoerythrin nanoparticles, which was denoted as PCNPs .

[0091] Under...

Embodiment 2

[0097] Example 2 Phycobiliprotein Nanoparticle Loaded Drugs

[0098] At normal temperature and pressure (15-35°C, 1 standard atmospheric pressure), prepare a water-soluble phycocyanin (PC) solution with a mass concentration of 20 mg / mL, and adjust the pH of the phycocyanin solution to 8.4. Slowly add 8 mL of anhydrous acetone dropwise under continuous stirring at a rate of 0.8 mL / min to desolvate the phycocyanin to form a nano-protein sol. After the dropwise addition, 23.5 μL of 8% (volume fraction) vanillin was added, mixed evenly under a magnetic stirrer, and the reaction was continued for 24 hours. After the reaction, the phycocyanin nanoparticles were centrifuged for 5 rounds (the centrifugation speed was 12000rpm, and each round lasted 10 minutes), and then resuspended with 8 mL of double distilled water to obtain a colloidal suspension of phycocyanin nanoparticles, which was recorded as PCNPs.

[0099] Dissolve 16mg of folic acid (folate, FA) in 0.8mL of 0.1N sodium hyd...

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Abstract

The invention relates to the field of biological medicines, and particularly provides an antitumor drug carrier and an application method thereof. The antitumor drug carrier is a phycobiliprotein nano particle. On the basis of the difference between normal cells and tumor cells, the phycobiliprotein nano particle without toxicity and immunogenicity is adopted as the antitumor drug carrier; the tumor-targeting capability is given; meanwhile, antitumor drugs are efficiently loaded; the antitumor drugs specifically reach the tumor lesion part; the treatment effects of high selectivity and low toxicity are realized; and the defects that a traditional cytotoxic drug is poor in selectivity and relatively high in toxic and side effect, and the drug resistance is easily generated are overcome. The preparation method provided by the invention is simple and feasible; and the prepared product can be stably stored in a water solution and is beneficial to storage.

Description

technical field [0001] The present invention relates to the field of biomedicine, and more specifically, relates to an antitumor drug carrier and its application method. Background technique [0002] Cancer is one of the major diseases that affect human health. According to a survey report published by the World Health Organization in February 2013, the number of people who died of cancer in the world in 2008 reached 7.6 million, and there are about 13 million new cancer patients every year. At present, cytotoxic drugs have been widely used in cancer chemotherapy, but these drugs are non-specific to cancer cells and healthy cells of the human body, so that while killing cancer cells, they also affect normal body functions. Therefore, increasing the local drug concentration and reducing the systemic toxicity is an urgent problem to be solved in tumor treatment. It is imperative to develop a tumor drug delivery system that is safe, non-toxic, tumor-specific and can efficientl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K45/00A61P35/00
Inventor 陈填烽郑文杰黄妍瑜
Owner GUANGDONG JINAN ESTABLISHED SELENIUM SOURCE NANO TECH RES INST CO LTD
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