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Extraction technology of active component from red onions

A technology of active ingredients and extraction process, applied in the field of extraction process of active ingredients, can solve the problems of small side effects, large toxic and side effects, single synthetic drug target, etc.

Inactive Publication Date: 2015-05-20
QINGDAO ZHONGREN ZHIYE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Synergistic treatment, with few side effects, overcomes the shortcomings of single synthetic drug target and large toxic side effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Get 4kg of dried red onions and grind them into coarse powder. Soak in 20L of 65% ethanol for 40 minutes, heat and reflux in a water bath at 85°C for 2 hours, filter and separate the extract and filter residue; reflux for 3 times, and finally combine the extracts. Recover under reduced pressure to a thick paste, add 3 L of water to suspend; extract with chloroform and n-butanol respectively to obtain 96 g of chloroform layer, 98 g of n-butanol layer, and 104 g of water layer. Using the blast fungus screening system, the chloroform layer with better activity against blast fungus was separated by silica gel column chromatography, medium and low pressure column chromatography and high performance liquid chromatography to obtain compound 1 (3g), compound 2 (5g), and compound 3 (4g). , Compound 4 (500 mg), Compound 5 (20 mg), Compound 6 (35 mg), Compound 7 (25 mg), Compound 8 (6 mg), Compound 9 (22 mg) and Compound 10 (12 mg). Among them, compounds 9 and 10 were isolated fr...

Embodiment 2

[0020] Get 4kg of dried red onions and grind them into coarse powder. Soak in 20L of 65% ethanol for 40 minutes, heat and reflux in a water bath at 85°C for 2 hours, filter and separate the extract and filter residue; reflux for 3 times, and finally combine the extracts. Recover under reduced pressure to a thick paste, add 3L of water to suspend; extract with chloroform and petroleum ether respectively to obtain 83g of chloroform layer, 35g of petroleum ether layer, and 145g of water layer. Using the blast fungus screening system, the petroleum ether layer with better activity against blast fungus was separated by silica gel column chromatography, medium and low pressure column chromatography and high performance liquid chromatography to obtain compound 1 (120mg), compound 2 (50mg), compound 3 (2g ), Compound 4 (20 mg), Compound 5 (33 mg), Compound 6 (20 mg), Compound 7 (140 mg), Compound 8 (10 mg), Compound 9 (50 mg). Among them, compound 9 was isolated from this genus for t...

Embodiment 3

[0022] Get 4kg of dried red onions and grind them into coarse powder. Soak in 20L of 65% ethanol for 30 minutes, heat and reflux in a water bath at 85°C for 2 hours, filter and separate the extract and filter residue; reflux for 3 times, and finally combine the extracts. Recover under reduced pressure to a thick paste, add 3 L of water to suspend; extract with chloroform, ethyl acetate, and n-butanol respectively to obtain 96 g of chloroform layer, 98 g of ethyl acetate, 98 g of n-butanol layer, and 104 g of water layer. Using the blast fungus screening system, the chloroform layer with better activity against blast fungus was separated by silica gel column chromatography, medium and low pressure column chromatography and high performance liquid chromatography to obtain compound 1 (100 mg), compound 2 (30 mg), and compound 3 (40 mg). , compound 4 (10 mg), compound 5 (3 g), compound 6 (200 mg), compound 7 (45 mg), compound 8 (12 mg), compound 9 (15 mg), compound 10 (60 mg), com...

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PUM

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Abstract

The invention relates to an extraction technology of active component from red onions. The extraction technology comprises main steps of pulverizing, extracting, condensing, extracting, and separating; and is characterized in that a pyricularia oryzae activity screening mode is used to assist the extraction technology so as to determine the optimal extraction part for active component extraction, and the active components are separated by silica gel column chromatography, medium-low pressure column chromatography, high performance liquid chromatography (HPLC), and the like. The provided extraction technology can be applied to massive production of active components with a stable quality from effective parts of red onions. The extraction technology has a high efficiency, and the obtained active component has the advantages of high purity and strong pharmaceutical effect.

Description

technical field [0001] The invention relates to a method for extracting natusin, in particular to an extraction process involving active ingredients of shallots. Background technique [0002] Shallot is a plant of the genus Shallot in the family Iridaceae, which is a folk herbal medicine; it is also known as red onion and small red garlic; the medicinal part is the whole plant and the bulb. Red onion is bitter and cool, and it is mainly used for rheumatic joint pain, bruises, sores, hematemesis, hemoptysis, dysentery and amenorrhea abdominal pain. So far, only a dozen compounds have been isolated from shallots, including naphthols, naphthones, anthraquinones and glucosides, which [0003] It is the main active ingredient of red onion, some of which have various pharmacological activities such as antibacterial, increasing coronary blood flow, inhibiting human immunodeficiency virus, and inhibiting human topoisomerase II. [0004] Red onion is of great development value in t...

Claims

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Application Information

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IPC IPC(8): C07G99/00
Inventor 谭超智
Owner QINGDAO ZHONGREN ZHIYE BIOTECH
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