Preparation method of anti-coagulating composite tubular scaffold with good compliance

Abstract

The invention provides a preparation method of an anti-coagulating composite tubular scaffold with good compliance. The tensile property of the tubular scaffold can be improved by the composite tubular scaffold by virtue of braided fabric; the tubular scaffold with sponge layers can be prepared by modification of silk fibroin and sulfated silk fibroin by polyethylene glycol diglycidyl ether; the compliance of the tubular scaffold is improved; the anti-permeability of the tubular scaffold can be improved by the silk fibroin and sulfated silk fibroin tubular scaffold with double sponge layers; meanwhile, the anti-coagulating property of the tubular scaffold can be improved by the sulfated silk fibroin; the composite tubular scaffold with good tensile property, compliance, anti-permeability and blood compatibility can be prepared by the combination; the pipe diameter is controllable; and the composite tubular scaffold can be applied to repair and reconstruction of a small-caliber blood vessel and building of hemodialysis vascular access. The preparation method is simple to operate and low in cost; and commercial production can be achieved.

Description

(1) Technical field [0001] The invention belongs to the field of biomedical materials, in particular to a composite tubular scaffold of silk fibroin and sulfated silk fibroin with double-layer sponge layers, which has good tensile properties, compliance, permeability resistance and blood compatibility . (2) Background technology [0002] In today's society, cardiovascular diseases seriously endanger human health, and vascular reconstruction plays a very important role in clinical surgery. More than 600,000 people around the world need to undergo various vascular surgeries every year, and most of them need suitable vascular grafts . At present, the vascular grafts used in clinical practice mainly include autologous blood vessels, allogeneic blood vessels and artificial synthetic blood vessels. Due to the limited source of autologous blood vessels and the rejection of allogeneic blood vessels, artificial vascular grafts have attracted much attention. At present, large-calibe...

AI Technical Summary

Problems solved by technology

Heparin is a common anticoagulant protein. Heparin can improve hemorheological properties after simple coating, but the binding strength is not enough, and it is easy to fall off, resulting in too fast release

In recent years, some scholars have used plasma irradiation to change the surface structure of materials, generate reactive groups on the surface of materials, and fix heparin on the surface of materials in the form of covalent bonds, but the anticoagulant effect is not satisfactory.

The scaffold material in the in vivo environment is exposed to various factors such as body fluids, organic macromolecules, enzymes, free radicals, cells, etc., and its biological environment is extremely complex. The method of surface modification can improve the long-term anticoagulant performance of the material Relatively limited, with the degradation of the stent material and the shedding of the surface modification layer, although its anticoagulant performance can meet the needs of large-caliber vessels with high blood flow and low resistance, it still cannot meet the requirements of small-caliber vessels with high anticoagulant performance. blood vessel needs

On the other hand, planting vascular endothelial cells on the inner surface of the scaffold material can form an anti-thrombotic surface layer, which can reduce the activity of prothrombin on the inner surface of tissue engineered blood vessels, thereby improving the hemocompatibility of the material, especially in small-diameter tissue engineered blood vessels. However, the insufficient preservation rate of endothelial cells after being washed by blood flow has always been a major problem for domestic and foreign scholars.

If the amount of infiltration is not enough, the surface blood diffusion cannot provide enough nutrients to the neointima, which in turn will lead to protein denaturation, and eventually cause fibroblasts and smooth muscle cells to proliferate and eventually lead to vascular stenosis and occlusion