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Anti-coagulation anti-bacterial biomedical material and preparation method thereof

A biomedical material and biomaterial technology, applied in the field of anticoagulant antibacterial biomedical materials and their preparation, can solve the problems of Staphylococcus aureus infection, failure, lack of antibacterial function, etc.

Inactive Publication Date: 2010-09-22
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem solved by the present invention is to propose an anticoagulant anticoagulant biomedical material and its preparation method, which overcomes the fact that the existing heparinized anticoagulant materials only have anticoagulant function and lack antibacterial function. In clinical application, such materials are prone to cause Staphylococcus aureus infection and fail

Method used

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  • Anti-coagulation anti-bacterial biomedical material and preparation method thereof
  • Anti-coagulation anti-bacterial biomedical material and preparation method thereof
  • Anti-coagulation anti-bacterial biomedical material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Dissolve 0.5g of silk fibroin (SF) in 50ml of 0.05M MES buffer solution with pH=5.5--6.5, and stir evenly for 1h.

[0027] 2) Dissolve 0.5g of heparin (heparin, Hep) in 50ml of MES buffer solution of 0.05M, pH=5.5--6.5, stir evenly for 30min, follow EDC:NHS:Hep-COOH=2:1:1 Add EDC and NHS at the same ratio, and react for 5--30 minutes to activate heparin.

[0028] 3) Add the above silk fibroin solution into the activated heparin solution, stir for 5 hours, and let it fully react to obtain heparinized silk fibroin material (Hep-SF).

[0029] 4) Dissolve 0.5g of chitosan (chitosan, CS) in 10ml of 0.5% acetic acid solution, and then dilute to 100ml with MES buffer.

[0030] 5) The above heparinized silk fibroin (Hep-SF) solution was gradually added dropwise to the chitosan solution, and after mixing for 2 hours, a milky white viscous suspension was formed.

[0031] 6) Put the suspension into a dialysis bag with a molecular weight cut-off of 3500, and dialyze in pure wa...

Embodiment 2

[0041] 1) Dissolve 0.5g of silk fibroin (SF) in 50ml of 0.05M MES buffer solution with pH=5.5--6.5, and stir evenly for 1h.

[0042] 2) Dissolve 0.5g of heparin (heparin, Hep) in 50ml of MES buffer solution of 0.05M, pH=5.5--6.5, stir evenly for 30min, follow EDC:NHS:Hep-COOH=2:1:1 Add EDC and NHS at the same ratio, and react for 5--30 minutes to activate heparin.

[0043] 3) Add the above silk fibroin solution into the activated heparin solution, stir for 5 hours, and let it fully react to obtain heparinized silk fibroin material (Hep-SF).

[0044] 4) Dissolve 1g of chitosan (chitosan, CS) in 20ml of 0.5% acetic acid solution, and then dilute to 100ml with MES buffer.

[0045] 5) Dissolve 0.5g of arginine (arginine, Arg) in 100ml of MES buffer solution, then add EDC and NHS according to the ratio of EDC:NHS:Arg-COOH=2:1:1, react for 15min for activation The carboxyl group of arginine, and then the chitosan solution was reacted with activated arginine to prepare a positively...

Embodiment 3

[0056] 1) Dissolve 0.5g of silk fibroin (SF) in 50ml of 0.05M MES buffer solution with pH=5.5--6.5, and stir evenly for 1h.

[0057] 2) Take 0.5g of heparin (heparin, Hep) and dissolve it in 50ml of MES buffer solution of 0.05M, pH=5.5--6.5, stir evenly for 30min, follow EDC:NHS:Hep-COOH=2:1:1 Add EDC and NHS at the same ratio, and react for 5--30 minutes to activate heparin.

[0058] 3) Add the above silk fibroin solution into the activated heparin solution, stir for 5 hours, let it fully react, and obtain the heparinized silk fibroin material (Heparin-silkfibroin, Hep-SF).

[0059] 4) Take 1g of chitosan (chitosan, CS) and 1g of sodium iodide (NaI) and react with 60ml of N-methylpyrrolidone (NMP) at 60°C for 2h under the catalysis of 60ml, then add 5ml, 10% NaOH and 5ml of methyl iodide, still React at 60°C for 2h.

[0060] 5) The obtained samples were washed three times in ethanol solution and three times in pure water, and finally the samples were freeze-dried to obtain ...

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Abstract

The invention relates to an anti-coagulation anti-bacterial biomedical material and a preparation method thereof in particular. The anti-coagulation anti-bacterial material is a composite material prepared by mixing a heparinized anti-coagulation material which is obtained by performing heparinization on an amidogen-containing natural biological material by a chemical covalent grafting method and an anti-bacterial material which takes chitosan as a substrate. The difunctional anti-coagulation anti-bacterial material of the invention has not only high blood compatibility, but also very good anti-staphylococcus aureus function. The novel material has double functions of anti-coagulation and antibacterium for the first time, and has a broad application prospect in clinical vascular repair.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to an anticoagulant and antibacterial biomedical material and a preparation method thereof. Background technique [0002] With the development of population aging process, the number of patients with cardiovascular diseases in our country is increasing year by year, so the demand for artificial blood vessel materials and anticoagulant medical materials is increasing day by day. Whether it is used as a vascular replacement material or a stent material inside a blood vessel, its blood compatibility is a prerequisite for determining whether it has clinical application value. Therefore, improving and improving the blood compatibility of materials is one of the important research directions in the field of vascular repair materials. At present, there are many methods for preparing anticoagulant materials, such as heparin immobilization, increasing the water content of mat...

Claims

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Application Information

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IPC IPC(8): A61L33/10A61L27/54
Inventor 张胜民王江林刘群
Owner HUAZHONG UNIV OF SCI & TECH
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