Method for preparing macromolecule resin type bilirubin sorbent

Abstract

The present invention features that the bilirubin adsorbent is synthesized by selecting well biocompatible polystyrene-divinylbenzene copolymer as carrier, PHEMA as coating agent, epichlorohydrin for activating the functional hydroxy radical of PHEMA, and glutaraldehyde as cross-linking agent for cross-linking functional cyclodextrin molecule. The bilirubin adsorbent has biocompatibility and adsorption capacity superior to active carbon and cationic resin adsorbing material, and has no adsorbent molecule falling resulting in immunological dysfunction. Animal experiment shows that the novel bilirubin adsorbent may be used to eliminate bilirubin from blood, blood plasma and albumin solution effectively.

Description

Technical field [0001] The invention belongs to the field of biological separation engineering, and relates to a new type of medical adsorbent, in particular to a bilirubin adsorbent for human hemoperfusion. Background technique: [0002] Bilirubin (BR) is a heme metabolite of senescent red blood cells. Bilirubin generally forms a water-soluble complex by binding to glucuronic acid in the human body. The content of bilirubin in normal human blood is generally 2-10mg / L. The metabolism of bilirubin is mainly completed in the liver. If liver metabolism is unregulated, bilirubin will accumulate in the blood. In severe cases, the concentration of bilirubin in plasma can be as high as 300-400mg / L. Excessive bilirubin becomes an endogenous toxin. Bile duct obstruction, acute and chronic icteric hepatitis, toxic hepatitis, and various hemolytic hepatitis can cause abnormal bilirubin metabolism in humans. Excessive free BR will deposit in tissues (especially in the brain), resulting in hi...

AI Technical Summary

Problems solved by technology

However, even with the continuous improvement of the coating technology of activated carbon, the effect of activated carbon adsorbents on removing BR is not very good, mainly because the inner pores of activated carbon itself are small and cannot sufficiently approach macromolecular substances such as BR.

[0006] Anion-exchange resins (strong and weak bases, polyvinylpyrimidine and other ammonium and amino-containing resins) are effective in removing BR, but they are not well-suited for hemoperfusion therapy because they simultaneously adsorb metal ions , and disrupt the electrolyte balance in the human blood