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Composite preparation capable of promoting restoration of skin wound due to diabetes and preparation method thereof

A compound preparation and skin wound technology, applied in the field of medicine, can solve the problems of increasing the risk of infection, inhibiting inflammatory response, and having little effect on skin wound treatment, and achieving the effect of promoting rapid healing and inhibiting inflammatory response.

Inactive Publication Date: 2014-12-31
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although traditional skin wound repair materials have a good therapeutic effect on ordinary skin wounds, they have little effect on the treatment of severe skin wounds such as diabetic skin ulcers. Restorative materials are not able to suppress the inflammatory response well
On the other hand, autologous keratinocytes and fibroblasts are usually selected when cells combined with biomaterials are used to treat severe skin wounds. However, the culture cycle of autologous keratinocytes and fibroblasts is long, and skin transplantation cannot be performed in time. Increased hospital stay and cost for patients, while also increasing the risk of infection

Method used

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  • Composite preparation capable of promoting restoration of skin wound due to diabetes and preparation method thereof
  • Composite preparation capable of promoting restoration of skin wound due to diabetes and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation and culture of BMSCs cells:

[0023] Choose 8-week-old healthy male C57BL / 6 mice (purchased from the Animal Experiment Center of Southern Medical University), kill them by dislocation, soak them in 75% ethanol for 5 minutes; take out the femurs and tibias under sterile conditions, and wash them with sterilized PBS buffer; Cut off both ends of the bone to expose the bone marrow cavity, use a 1ml syringe to draw culture solution (DMEM containing 10v / v% fetal bovine serum, 1 v / v% double antibody, 1 v / v% glutamine) into the bone marrow cavity, and flush out Bone marrow, wash 3-5 times repeatedly until the bone marrow cavity turns white; transfer the culture medium containing bone marrow cells into a centrifuge tube, centrifuge at 1000rpm for 5min, discard the supernatant, and resuspend in L-DMEM medium containing 10% fetal bovine serum Cells were transferred to a culture dish and placed at 37°C, 5% CO 2 Incubate in an incubator for primary culture; change the m...

Embodiment 2

[0025] Preparation of thermosensitive hydrogel-BMSCs composite biological preparation 1#:

[0026] After the third passage BMSCs grow to 80%, replace with serum-free DMEM medium, 37°C, 5% CO 2 After culturing in the incubator for 6 hours, digest with 0.25% trypsin, centrifuge, discard the supernatant, resuspend with DHanks solution and count, collect the cell pellet by centrifugation, add thermosensitive hydrogel according to the cell count result, mix well, The final concentration of cells was 1×10 5 Each / ml compound biological preparation is ready for use.

Embodiment 3

[0028] Preparation of thermosensitive hydrogel-BMSCs composite biological preparation 2#:

[0029] After the 6th passage BMSCs grew to 60%, they were replaced with serum-free DMEM medium at 37°C, 5% CO 2 After culturing in the incubator for 12 hours, digest with 0.25% trypsin, centrifuge, discard the supernatant, resuspend and count with DHanks solution, collect the cell pellet by centrifugation, add thermosensitive hydrogel according to the cell count result, mix well, The final concentration of cells was 1×10 7 Each / ml compound biological preparation is ready for use.

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Abstract

The invention discloses a composite preparation capable of promoting restoration of skin wound due to diabetes and a preparation method thereof. The method successfully prepare a temperature sensitive hydrogel-BMSCs composite biological preparation, the preparation is used for restoration of skin wound due to diabetes mice, and the result displays that the composite preparation can inhibit the skin wound inflammation due to diabetes mice and can promote rapid heal of wound. The composite preparation provides important drug reference for treating skin wound of clinical diabetes and other chronic skin ulcer.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a compound preparation for promoting diabetic skin wound repair and a preparation method thereof. Background technique [0002] Diabetes mellitus is a chronic metabolic disease characterized by persistent hyperglycemia. The greatest threat of diabetes to patients comes from its complications, such as retinopathy, diabetic nephropathy, neuropathy, and refractory wounds. Among them, the repair of diabetic refractory wounds and the treatment of spontaneous skin ulcers not only plague clinicians, but also are major problems faced by the medical field. Complications of diabetes have become the main cause of disability and death of diabetic patients, and also the main factor of increasing medical expenses. In recent years, many new theories and viewpoints have been put forward for the mechanism of diabetic skin wound healing, mainly focusing on the following aspects: 1. ...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K47/32A61K9/06A61P17/02A61P3/10
Inventor 张琳张璐陈世萱
Owner SOUTHERN MEDICAL UNIVERSITY
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