Heat-shock transcription factor gene AtHSFA1d, and coding protein and application thereof

Abstract

The invention belongs to the fields of plant molecular biology and transgenic correlation techniques, and particularly relates to verification of high-temperature-resistant functions of Arabidopsis thaliana heat-shock transcription factor gene AtHSFA1d and application of the gene in culture of new species of high-temperature-resistant plants. By using the model plant Arabidopsis thaliana as the material, the AtHSFA1d gene T-DNA insertion homozygous mutant strain system salk_058618 is utilized to research the functions of the AtHSFA1d gene in the plant high-temperature-resistance aspect. The experimental result shows that the AtHSFA1d gene knockout mutant is sensitive phenotype under heat shock stress and has obvious or extremely obvious differences from the wild type in the aspects of survival rate, electric conductivity, osmotic pressure, malondialdehyde, SOD (superoxide dismutase), POD (peroxidase) enzyme activities and other physiologic indexes, which indicates that the AtHSFA1d gene has important actions on signal transmission paths under plant response heat-shock stress. The overexpression of the gene is estimated to enhance the heat resistance of the plants and have great economic value in plant stress-tolerant molecule breeding.

Description

Technical field: [0001] The invention relates to the field of plant molecular biology, in particular to the verification of the new high-temperature resistance function of Arabidopsis thaliana heat shock transcription factor (Heat Shock Transcription Factors, HSFs) family member AtHSFA1d, and the application of the gene in breeding new high-temperature-resistant plant varieties. Background technique: [0002] The change of temperature has an important impact on the growth and development of plants. Appropriate temperature can ensure or promote the normal growth and development of plants, while unsuitable temperature conditions will inhibit the growth of plants and even cause plant death. Since the 20th century, with the rapid growth of world population and the continuous deepening of industrialization, the greenhouse effect has become more and more serious, and the gradual increase of global temperature has become an important limiting factor for the normal growth, developmen...

AI Technical Summary

Problems solved by technology

But so far there is no report on the heat stress response of a single gene of the HSFA1s subfamily, which makes it difficult for people to understand the functions and interactions of each member of the subfamily, and also makes it difficult to breed new varieties of high-temperature resistant crops through transgenic means. More cumbersome, polygenic transformation not only increases the technical difficulty, but also increases the uncertainty of offspring functions and phenotypes, and related theoretical and technical research often becomes at a loss as a result.

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