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Synthesis method of lectin-iron core gold shell nanoparticles for tumor MRI/CT dual-mode diagnosis

A technology of nano-particles and synthesis methods, applied in preparations for in vivo tests, pharmaceutical formulations, preparation of X-ray contrast agents, etc., can solve the problems of long production cycle and complicated process of monoclonal antibodies, and achieve long blood circulation time , the synthesis method is simple, the effect of good stability

Inactive Publication Date: 2013-09-11
CHANGCHUN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the preparation cycle of monoclonal antibody is long and complicated

Method used

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  • Synthesis method of lectin-iron core gold shell nanoparticles for tumor MRI/CT dual-mode diagnosis
  • Synthesis method of lectin-iron core gold shell nanoparticles for tumor MRI/CT dual-mode diagnosis
  • Synthesis method of lectin-iron core gold shell nanoparticles for tumor MRI/CT dual-mode diagnosis

Examples

Experimental program
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preparation Embodiment 1

[0020] Preparation Example 1: Synthesis of Wheat Germ Agglutinin (WGA)-Gold Shell Iron Nuclear Magnetic Nanoparticles

[0021] Combine ferric chloride and ferric chloride with Fe Ⅱ / Fe Ⅲ The solution with a concentration of 0.5 molar ratio is added dropwise to the 1.5M NaOH solution and vigorously stirred according to the volume ratio of 1:10. The resulting black precipitate is collected with a magnet, washed with water three times, and neutralized with 0.01M hydrochloric acid The negative ions in the precipitate are collected with a magnet and washed three times with water to obtain fresh ferroferric oxide nanoparticles, which are dissolved in 0.01M nitric acid solution, heated at 90-100°C and stirred vigorously for several hours until they are completely oxidized to brown Red γ-Fe 2 O 3 After the solution is cooled to room temperature, it is collected by centrifugation, washed with water, and washed once with 0.1M tetramethylammonium hydroxide to dissolve the precipitate. The...

preparation Embodiment 2

[0023] Preparation Example 2: Synthesis of Castor Lectin (RCA)-Gold Shell Iron Nuclear Magnetic Nanoparticles

[0024] Combine ferric chloride and ferric chloride with Fe Ⅱ / Fe Ⅲ A solution with a concentration of 0.5 molar ratio is added dropwise to the 1.5M NaOH solution and vigorously stirred according to the volume ratio of 1:10. The resulting black precipitate is collected with a magnet, washed with water three times, and neutralized with 0.01M hydrochloric acid The negative ions in the precipitate are collected with a magnet and washed three times with water to obtain fresh ferroferric oxide nanoparticles. Dissolve them in a 0.01M nitric acid solution, heat them at 90-100°C and stir vigorously for several hours until they are completely oxidized to brown Red γ-Fe 2 O 3 After the solution is cooled to room temperature, it is collected by centrifugation, washed with water, and washed once with 0.1M tetramethylammonium hydroxide to dissolve the precipitate. The obtained ferr...

preparation Embodiment 3

[0026] Preparation Example 3: Synthesis of Concanavalin A (ConA)-Gold-shell Iron Nuclear Magnetic Nanoparticles

[0027] Combine ferric chloride and ferric chloride with Fe Ⅱ / Fe Ⅲ The solution with a concentration of 0.5 molar ratio is added dropwise to the 1.5M NaOH solution and vigorously stirred according to the volume ratio of 1:10. The resulting black precipitate is collected with a magnet, washed with water three times, and neutralized with 0.01M hydrochloric acid The negative ions in the precipitate are collected with a magnet and washed three times with water to obtain fresh ferroferric oxide nanoparticles, which are dissolved in 0.01M nitric acid solution, heated at 90-100°C and stirred vigorously for several hours until they are completely oxidized to brown Red γ-Fe 2 O 3 After the solution is cooled to room temperature, it is collected by centrifugation, washed with water, and washed once with 0.1M tetramethylammonium hydroxide to dissolve the precipitate. The obtai...

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Abstract

The invention relates to a synthesis method of lectin-iron core gold shell nanoparticles for tumor MRI / CT dual-mode diagnosis, and belongs to the technical field of medical diagnostic devices. The synthesis method comprises: a, adding an iron salt solution comprising ferrous chloride and ferric chloride according to a Fe II / Fe II molar ratio of 0.5 to a 1.5 M NaOH solution according to a volume ratio of 1:10 in a dropwise manner, vigorously stirring, collecting the produced black precipitate by using a magnet, carrying out water washing, collecting, adopting tetramethylammonium hydroxide to wash, dissolving the precipitate, diluting the obtained ferric oxide nanoparticle solution to 1.1 mM with water, adding sodium citrate, stirring, and sequentially and alternately adding hydroxylamine hydrochloride and chlorauric acid to obtain gold coated ferric oxide core-shell structure nanoparticles; and b, carrying out a reaction of lectin and NHS-PEG-S-S-PEG-NHS, carrying out a reaction of the PEG in a K2CO3 solution for at least 4 h, and continuously carrying out a reaction of the Fe2O3@Au nanoparticles and PEG-SH5000 for more than 1 h. The lectin-iron core gold shell nanoparticles have beneficial effects of small particle size, simple synthesis method, good stability, long blood circulation time, and no immunogenicity. In addition, specific lectin binding target molecules can be screened based on different tumor cells.

Description

technical field [0001] The invention belongs to the technical field of medical diagnostic equipment, and relates to an improvement of a method for synthesizing lectin-iron oxide core gold-shell nanoparticles used for MRI / CT dual-mode diagnosis of tumors. technical background [0002] In recent years, with the development of nanoscience and nanotechnology, magnetic nanoparticles have shown a wide range of application prospects. Such as biosensing, disease diagnosis, drug and gene delivery, especially in the field of molecular imaging, used as contrast agent for various imaging applications. Superparamagnetic iron oxide nanoparticles for magnetic resonance imaging (MRI) T 2 Time has a significant effect. Effectively shorten T 2 time so that the resulting T 2 Weighted images are darkened, exhibiting a negative enhancement effect. But none of these traditional contrast agents can provide all the structural, functional and molecular information about tissues. In order to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/14A61K49/04
Inventor 何秀霞王振新于源华张小丽章俊平
Owner CHANGCHUN UNIV OF SCI & TECH
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