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Bacillus subtilis YB-04 and microorganism preparation thereof, and application of bacillus subtilis YB-04 in straw degradation

A Bacillus subtilis, YB-04 technology, applied in the field of microorganisms, can solve problems such as affecting the quality of sowing, emergence and growth of seedlings, the impact of the next crop, and slow decomposition of straw.

Active Publication Date: 2013-08-21
河南新仰韶生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under natural conditions, the straw decomposes slowly. If a large amount of straw is returned to the field, the straw cannot be fully decomposed, which will affect the quality of sowing, emergence and growth of seedlings.
Straw mulching will also provide a place for overwintering for pests and diseases, which will affect the next crops

Method used

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  • Bacillus subtilis YB-04 and microorganism preparation thereof, and application of bacillus subtilis YB-04 in straw degradation
  • Bacillus subtilis YB-04 and microorganism preparation thereof, and application of bacillus subtilis YB-04 in straw degradation
  • Bacillus subtilis YB-04 and microorganism preparation thereof, and application of bacillus subtilis YB-04 in straw degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation and screening of Bacillus subtilis YB-04

[0023] Sample collection: The samples were collected from rotting corn and wheat straw in several fields at the Yuanyang Base of Henan Academy of Agricultural Sciences (collection time: June 2010-October 2010; specific collection location: Yuanyang Base of Henan Academy of Agricultural Sciences, Collector: Xie Maofang), brought back in a fresh-keeping bag.

[0024] Isolation and purification of Bacillus subtilis: take the rotten straw collected, weigh 1g into a conical flask containing 100mL of sterile water, and shake fully on a constant temperature oscillator at 28°C for 2h. 10 by dilution coating plate method -1 -10 -7 The samples were spread on LB plates, cultured at 37°C for one day, and purified on LB plates according to the colony morphology. Place the culture medium upside down at 37°C for constant temperature cultivation overnight, and finally pick a single colony similar in shape, color, and siz...

Embodiment 2

[0028] Example 2 Identification of Bacillus subtilis YB-04

[0029] （1） Morphological and Physiological and Biochemical Taxonomic Identification The YB-04 strain was identified by colony morphology observation and microscopic observation, and its species was determined by searching the literature. Individual morphological characteristics, including Gram staining reaction, spore observation, cell size, etc.; group morphology observation, colony shape, colony surface characteristics, colony edge, colony color, and whether soluble pigments are secreted; physiological and biochemical tests. Peroxidase determination, V.P test, sugar fermentation test, starch hydrolysis test, gelatin liquefaction, citrate utilization, indole test, salt tolerance test, etc.

[0030] （2）Molecular identification: Genomic DNA extraction refers to the following steps: ① Cultivate 5 mL of bacterial culture to saturation, and centrifuge 1.2 mL of the culture at 12,000 rpm for 1 min. ② Add 500 μL of s...

Embodiment 3

[0038] Embodiment 3 YB-04 microbial inoculum preparation method

[0039] (1) Strain cultivation and preparation Inoculate Bacillus subtilis YB-04 on the slant medium of TSA test tube, and culture at 28-35°C for 24-36 hours to obtain the bacteria;

[0040] (2) Seed solution preparation: Introduce the test tube bacteria prepared in step (1) into TSB culture medium, and shake and culture at 28-32°C for 12-16 hours to make seed solution;

[0041] (3) Preparation of fermentation medium: Glucose, corn flour, soybean cake powder, and dipotassium hydrogen phosphate were added to water to prepare a culture solution containing 1.2% glucose, 1.8% corn flour, 3% soybean cake powder, and 0.2% dipotassium hydrogen phosphate;

[0042] (4) Liquid fermentation production After sterilizing the fermentation equipment and culture medium, inoculate the seed liquid into the culture liquid prepared in step (3) at an inoculation amount of 5%, at 27-34°C, at a speed of 180rpm, and at a pH of 8.0 Afte...

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Abstract

The present invention relates to a strain of Bacillus subtilis YB-04 and a preparation method of Bacillus subtilis YB-04 agent, wherein the preservation number of the Bacillus subtilis YB-04 is CGMCC NO.6497. According to the present invention, the Bacillus subtilis YB-04 provides a significant straw degradation effect, provides significant bacterial inhibition effects on wheat root take-all diseases, sheath blight and the like, and presents broad-spectrum bacterial inhibition effects, wherein a degradation rate can be 49% when the Bacillus subtilis YB-04 separately acts on straws, when the Bacillus subtilis YB-04 and fungi mixedly act on straws to achieve moistening and rotten surface of the straw, a degradation rate achieves the maximal value 59%, and a degradation effect of the experiment combination is far superior to a degradation effect of the commercially available bacterial agent; and the Bacillus subtilis YB-04 agent further presents broad-spectrum bacterial inhibition effects, wherein the Bacillus subtilis YB-04 agent provides good bacterial inhibition effects for cotton verticillium dahlia, wheat take-all pathogen, wheat sheath blight and other rhizosphere plant diseases, straws can be degraded, rhizosphere pathogenic bacteria can be inhibited, rhizosphere microorganism community can be regulated, and good development utilization prospects are provided.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis YB-04, its microbial preparation and its application in straw degradation. Background technique [0002] In recent years, more and more attention has been paid to returning straw to the field. On the one hand, large-scale agricultural production requires straw to be processed nearby to save labor; on the other hand, long-term simple application of chemical fertilizers is not conducive to the development of soil fertility. Crop stalks are rich in organic nutrients such as nitrogen, phosphorus, and potassium. Returning straw to the field will increase the content of soil organic matter, improve soil structure, and improve soil fertility. A large number of studies have shown that returning straw to the field has a significant impact on soil nutrients and organic matter content. improve. However, under natural conditions, straw decomposes slowly. If a larg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/00A01N63/02A01P3/00C12R1/125
Inventor 杨丽荣薛保国全鑫谢茂芳刘发魁王更新赵献林马雪皎吴坤马毅辉武超
Owner 河南新仰韶生物科技有限公司
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