Induction medium for inducing human adipose tissue-derived stromal cells as nerve cells and method

Abstract

The invention belongs to the technical field of induction and differentiation of stem cells and in particular relates to a medium for inducing differentiation and a method. An induction medium for inducing human adipose tissue-derived stromal cells as nerve cells consists of a solution A, a solution B and a solution C and is prepared by adopting a method comprising the following steps of: preparing the solution A by dissolving 8-12 micrograms of bFGF (Basic Fibroblast Growth Factor) in 500ml of a serum-free medium of the human adipose tissue-derived stromal cells, filtering and degerming to acquire the solution A; preparing the solution B by adding 10-20ml of injection of salviae miltiorrhizae composite into 490-480ml of the serum-free medium of the human adipose tissue-derived stromal cells to acquire the solution B; and preparing the solution C by dissolving 4-8 micrograms of bFGF, 4-8 micrograms of EGF (Epidermal Growth Factor), 40-60 micrograms of BDNF (Brain Derived Neurotrophic Factor) and 80-120 micrograms of GM1 (gangliosides) in 500ml of the serum-free medium of the human adipose tissue-derived stromal cells to acquire the solution C. By adopting the medium for inducing human adipose tissue-derived stromal cells as nerve cells disclosed by the invention, the human adipose tissue-derived stromal cells are differentiated into the nerve cells by adopting the injection of salviae miltiorrhizae composite and combining the growth factors EGF, bFGF, BDNF and GM1; and the induction medium disclosed by the invention is non-toxic in selected induction components, high in induction efficiency and short in induction time; and the exclusion and ethics problems do not exist in the inducted cells after transplanting, so that the induction medium is high in safety.

Description

technical field [0001] The invention belongs to the technical field of induction and differentiation of stem cells, and in particular relates to a medium and a method for inducing differentiation. Background technique [0002] Nervous system damage and neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease, Huntington's disease, etc., have always been a problem that plagues human health, and cell transplantation is the hope for curing such diseases. At present, embryonic stem cells and neural stem cells are mainly used for transplantation to treat central nervous system injuries. However, due to difficulties in donor sources, ethical objections, immune rejection, and difficulties in the survival of transplanted cells, the development space is limited. Adipose-derived mesenchymal stem cells have a wide range of sources, are easy to obtain, are convenient for autologous transplantation, and have strong proliferation ability. They can always maintain their...

AI Technical Summary

Problems solved by technology

[0009] Although the traditional chemical inducer method can efficiently and quickly differentiate adipose-derived mesenchymal stem cells into nerve cells, the cell death rate is relatively high (chemical inducers have certain toxicity, BHA, DMSO, etc. can cause cell mutation, and are used for cell transplantation. There is a risk of tumorigenicity); although the growth factor induction method has a high cell survival rate after induction, the induction time is long and the induction efficiency is low

Gene transfection induces potential risk of cancer due to genetic alterations

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