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A kind of preparation method of collagen membrane

A collagen membrane and collagen technology, which can be used in food preparation, peptide/protein components, medical preparations containing active ingredients, etc. Toxicity, promotion of cell proliferation, hemolytic and antigenic low effects

Active Publication Date: 2015-12-02
SHANGHAI JINGZE PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the interaction between PEO and collagen, the fiber has good mechanical properties; however, the introduction of other polymer materials has many limitations in its application, and it also greatly reduces the safety of the use of collagen membranes; in addition, the application No.: 200910209509.6, Invention name: "Preparation method of collagen ultrafine fiber membrane material" reported in the patent application document that the mixture of phosphate and absolute ethanol was used as the collagen electrospinning solution, although the spinnability of collagen can be achieved technically , but it is not easy to desalt in the later stage, which not only increases the production cost, but also limits the application

Method used

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  • A kind of preparation method of collagen membrane
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  • A kind of preparation method of collagen membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 The preparation method of nano-collagen film of the present invention

[0044] 1. Preparation method

[0045](1) Accurately weigh 1.3531g of collagen sponge, put it into a small beaker, draw 12.3ml of hexafluoroisopropanol solution into the small beaker, stir on a magnetic stirrer at 25°C for about 6 hours until the solution is clear and transparent, and prepare the concentration Collagen hexafluoroisopropanol solution of 110mg / ml;

[0046] (2) Take 12ml of hexafluoroisopropanol solution in 5ml glass syringes, connect the needle, and fix it on the micro-injection pump. Fix the aluminum foil of width)×30 (length) on the flat receiver, ground it, and set the distance between it and the needle at 12.5cm; set the voltage at 16.0KV, conduct electrospinning, and dry in a vacuum oven at 25°C for 2 hours , the collagen fiber electrospun membrane was removed from the aluminum foil to obtain the nano-collagen membrane.

[0047] The resulting collagen film was meas...

Embodiment 2

[0049] Embodiment 2 The preparation method of double-layer collagen film of the present invention

[0050] The nano-collagen electrospun membrane obtained in Example 1 was fixed on a flat plate receiver;

[0051] Prepare a collagen hexafluoroisopropanol solution with a concentration of 50mg / ml, take a 5ml glass syringe to absorb the collagen solution in hexafluoroisopropanol solution, connect the needle, and fix it on the micro-injection pump, connect the needle to the high-voltage DC power supply Connected, the propulsion speed is 1.2ml / h, take 20 (width) × 30 (length) aluminum foil and fix it on the flat receiver, ground it, set the distance between it and the needle to 12.5cm; set the voltage to 16.0KV, use Collagen hexafluoroisopropanol solution is sprayed by electrospraying to form a layer of nanoparticles on the collagen electrospun membrane, and dried in a vacuum oven at 25°C for 2 hours.

[0052] Under the electron microscope, it can be seen that the collagen film is ...

Embodiment 3

[0054] Embodiment 3 The preparation method of double-layer collagen film of the present invention

[0055] The nano-collagen electrospun membrane obtained in Example 1 was fixed on a flat plate receiver;

[0056] Prepare a collagen hexafluoroisopropanol solution with a concentration of 80mg / ml, take a 5ml glass syringe to draw the hexafluoroisopropanol solution collagen solution, connect the needle, and fix it on the micro-syringe pump, connect the needle to the high-voltage DC power supply Connected, the propulsion speed is 0.5ml / h, take 20 (width) × 30 (length) aluminum foil and fix it on the flat receiver, ground it, set the distance between it and the needle to 12.5cm; set the voltage to 15.0KV, use Collagen hexafluoroisopropanol solution was sprayed by electrospraying to form a layer of nanoparticles on the collagen electrospun membrane, and dried in a vacuum oven at 25°C for 2 hours.

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Abstract

The invention discloses a preparation method of a nano collagen membrane. The method includes the following steps of step one, dissolving collagen in hexafluoroisopropanol to obtain an 80-120mg / ml of collagen hexafluoroisopropanol solution; and step two, subjecting the collagen hexafluoroisopropanol solution obtained from the step one to electrostatic spinning with voltage of 15-19 KV, the receiving distance of 12.5-15cm and the advance speed of 0.5-1.2 ml / h so as to remove hexafluoroisopropanol to obtain the nano collagen membrane. The invention further discloses a preparation method of a double-layer collagen membrane, the prepared collagen membrane and application of the collagen membrane. The collagen membrane is excellent in mechanical property, free of cytotoxicity, low in hemolysis and antigenicity and high in biological activity.

Description

technical field [0001] The invention relates to a preparation method and application of a collagen film. Background technique [0002] Collagen is a natural protein that widely exists in animal skin, bone, cartilage, teeth, tendons, ligaments, and blood vessels. It is an extremely important structural protein in connective tissue and plays a role in supporting organs and protecting the body. Collagen is generally white, transparent, unbranched fibrils, mainly found in raw hides and secondarily in bones. The collagen family includes 19 kinds of collagen and more than 10 kinds of collagen-like proteins. They are wound by three left-handed helical polypeptide chains, right-handed, forming a rope-like right-handed superhelical structure. The common characteristics of their I-level structures are: two There is a glycine in the amino acid. In the Y-Gly-X-Y-sequence, the X and Y positions are respectively proline and hydroxyproline to limit the rotation of the polypeptide chain. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/30D04H1/728D04H13/00D01D5/00A23L1/305A61K38/39A61P17/02A61P7/04A61L27/24
Inventor 孙莉嫚彭红卫陈春会李好雨刘富俊张风豪朱小舟
Owner SHANGHAI JINGZE PHARMA CO LTD
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