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Bipolar electrode-paper-based microfluidics type chip and preparation method thereof

A microfluidic chip and bipolar electrode technology are applied in the field of microfluidic analysis to achieve the effects of low cost, fast analysis speed and easy portability

Inactive Publication Date: 2013-04-03
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bipolar electrode chip devices reported internationally are mainly glass and PDMS, but the paper-based microfluidic-bipolar electrode electrophoresis separation chip and its preparation method disclosed in this patent have not been reported yet.

Method used

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  • Bipolar electrode-paper-based microfluidics type chip and preparation method thereof
  • Bipolar electrode-paper-based microfluidics type chip and preparation method thereof
  • Bipolar electrode-paper-based microfluidics type chip and preparation method thereof

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Effect test

Embodiment 1

[0038] A. Paper-based cover sheet production

[0039] according to figure 1 (B) Dilute the photoresist SU-82025 and SU-82000 diluent according to the volume ratio of 1:6, spread 0.5ml of the diluted photoresist evenly on the 5cm diameter chromatographic paper, and dry at room temperature for 2min Afterwards, directly align the mask film with the chromatographic paper on a photolithography machine (OL-2Mask Aligner, AB-M, Inc) and expose for 30s, develop with acetone for 5min, to obtain a paper cover sheet with microchannels;

[0040] B. Production of paper-based substrates

[0041] press figure 1 As shown in (c), use ordinary A4 paper cut into a certain size of paper as a mask, and cut off the electrode area of ​​the paper according to the size and shape of the electrode designed on the chromatographic paper, and place the finished paper mask on the chromatogram On the paper, magnetron sputtering tin indium oxide film is used to obtain 100nm thick nano-electrodes and bipolar electr...

Embodiment 2

[0045] A. Paper-based cover sheet production

[0046] according to figure 1 (B) Dilute the photoresist SU-82150 and chloroform in a volume ratio of 1:4, and evenly spread 0.5ml of the diluted photoresist on a 5cm diameter chromatographic paper. After drying at room temperature for 2 minutes, directly Align the mask film with the chromatographic paper on a photolithography machine (OL-2Mask Aligner, AB-M, Inc) and expose for 30s, and develop with acetone for 5min to obtain a paper cover film with microchannels;

[0047] B. Production of paper-based substrates

[0048] press figure 1 As shown in (c), use ordinary A4 paper cut into a certain size of paper as a mask, and cut off the electrode area of ​​the paper according to the size and shape of the electrode designed on the chromatographic paper, and place the finished paper mask on the chromatogram On the paper, the Au film is sputtered by magnetron to obtain 100nm thick nano-electrodes and bipolar electrodes; the bipolar electrode ...

Embodiment 3

[0053] A. Paper-based cover sheet production

[0054] according to figure 1 (B) Dilute the photoresist SU-8100 and cyclopentanone in a volume ratio of 1:8, and evenly spread 0.5ml of the diluted photoresist on a 5cm diameter chromatographic paper. After drying at room temperature for 2 minutes, Directly align the mask film with the chromatographic paper on a photolithography machine (OL-2Mask Aligner, AB-M, Inc) and expose for 30s, and develop with acetone for 5min to obtain a paper cover sheet with microchannels;

[0055] B. Production of paper-based substrates

[0056] press figure 1 As shown in (c), use ordinary A4 paper cut into a certain size of paper as a mask, and cut off the electrode area of ​​the paper according to the size and shape of the electrode designed on the chromatographic paper, and place the finished paper mask on the chromatogram On the paper, magnetron sputtering platinum film is used to obtain 100nm thick nano-electrodes and bipolar electrodes; the bipolar e...

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Abstract

The invention discloses a bipolar electrode-paper-based microfluidics type chip and a preparation method of the bipolar electrode-paper-based microfluidics type chip. The chip comprises a paper substrate, a paper cover plate, a nanometer electrode and a bipolar electrode, wherein the nanometer electrode and the bipolar electrode are deposited on the surface of the paper substrate; the nanometer electrode is used as a driving unit used for electroosmotic flow in a microfluidic channel and electrochemistry of the bipolar electrode; the bipolar electrode is used a non-uniform field which enables the components to be highly concentrated in the microfluidic channel, and the detected components are subjected to oxidation-reduction reaction at two ends of the bipolar electrode. The invention discloses a method for producing the chip through three different photoresists and diluents. The bipolar electrode-paper-based microfluidics type chip is light in weight, convenient to carry, low in cost and disposable; the required sample is small in size and fast to be analyzed; and the bipolar electrode-paper-based microfluidics type chip is applicable to detection on complex samples of foundations or on-board spaceship life science instruments.

Description

Technical field: [0001] The invention relates to a bipolar electrode-paper-based microfluidic chip and a preparation method thereof, belonging to the technical field of microfluidic analysis. Background technique: [0002] Since the concept of micro total analysis system was put forward in the 1990s, microfluidic chip as the core technology has become one of the fastest growing fields in the world. Paper-based microfluidics is a new direction in the research of microfluidic chips in recent years. It uses filter paper as the chip material, using photolithography, plotter technology, inkjet printing technology, wax printing technology, paper cutting, screen printing, flexographic printing, printing PDMS and plasma oxidation, laser processing, origami, etc. Used alone to make paper-based microfluidic chips, they have been successfully used in colorimetric analysis, electrochemical analysis, biological sample analysis and other fields. Because it has the advantages of light weight,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
Inventor 梁恒欧阳良飞刘倩杜芳马国营
Owner XI AN JIAOTONG UNIV
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