Tissue engineered osteochondral composite stent and preparation method thereof

Abstract

A tissue engineered osteochondral cartilage composite stent and a preparation method thereof belong to the technical field of tissue engineering and bio-manufacturing. The preparation method comprises the following steps of: simulating natural materials and structures of osteochondral cartilage; preparing a cartilage stent with an oriented structure by applying the principle of oriented crystallization; preparing a core-spun structural stent by applying a low-temperature deposition manufacturing process based on the rapid forming technique and designing a special extrusion and injection device with a core-spun sprayer; and connecting the oriented structural stent and the core-spun structural stent in a dissolution-adhesion method so as to construct the tissue engineered osteochondral cartilage composite stent with the structure of oriented structural cartilage and core-spun structural bone. According to the tissue engineered osteochondral cartilage composite stent and the preparation method thereof, with respect to the defect treatment of articular cartilages, a material with biological activity is adopted and the osteochondral cartilage composite stent with a special bionic structure is manufactured; the cell implanting density and depth of the stent can be improved, and the simultaneous regeneration of bones and cartilage tissues can be promoted with respect to the different functional regional characteristics of the osteochondral cartilage, so that the repairing effect of osteochondral cartilage defects is improved.

Description

technical field [0001] The invention relates to a preparation method of a tissue-engineered osteochondral composite bracket, which belongs to the technical field of tissue engineering and biomanufacturing. Background technique [0002] Articular cartilage is a specialized connective tissue with biophysical properties such as toughness, good elasticity, and low coefficient of friction. Trauma and joint inflammation can easily lead to articular cartilage defects and affect the stability of the joints, resulting in the occurrence of osteoarthritis and joint degenerative lesions. Such lesions can not only make people lose their ability to work, but have even become one of the main causes of physical disabilities. one. [0003] The osteochondral complex tissue at the joint end of adult can be divided into hyaline cartilage layer, calcified layer and subchondral bone from the depth direction of the joint. Each layer is connected with a unique composition and structure and perform...

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Problems solved by technology

[0008] 1. When using tissue engineering to simply repair articular cartilage defects, the healing interface between the graft body and the transplantation bed is the cartilage-cartilage and cartilage-bone interface. The integration of these two interfaces is slow, and the new tissue is formed on the surface of the transplantation bed during the repair process. Unstable, prone to repair failure

[0009] 2. At present, in the tissue engineering repair process, the proportion of hyaline cartilage in the cartilage tissue is relatively small, and the physiological functions and mechanical properties of the composite tissue cannot be maintained for a long time. The materials and construction methods of the cartilage scaffold are not bionic enough. The proportion of hyaline cartilage in cartilage tissue is small, and the physiological function and mechanical properties of the composite tissue cannot be maintained for a long time

[0010] 3. The hydrophilicity and compatibility of osteochondral scaffolds are not ideal enough. At present, the methods to improve the hydrophilicity of scaffolds in osteochondral tissue engineering research are mainly surface modification or intermittent addition of highly hydrophilic materials during scaffold construction. The modified material is not easy to distribute evenly, and the complete pore structure cannot be maintained after the material is modified.

Dietmar W. et al. (Shao X X, Hutmacher D W, Ho S T, et al. Evaluation of a hybrid scaffold / cell construct in repair of high-load-bearing osteochondral defects in rabbits. BIOMATERIALS, 2006, 27(7) :1071~1080) in 2005 constructed a medical-grade polycaprolactone / fibrin glue osteochondral integrated scaffold inoculated with BMSCs on both sides and filled the animal defect with a diameter of 4mm and a depth of 5mm. The samples showed good cartilage repair after 3 months Characteristics, mechanical experiments prove that PCL is a better subchondral bone scaffold material, but fibrin glue is not suitable as a cartilage scaffold due to its weak mechanical properties

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