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Pleurotus eryngii polysaccharide capable of reducing accumulation of foam cell lipid and preparation method thereof

A technology for the accumulation of polysaccharides and lipids in Pleurotus eryngii, applied in food preparation, anti-toxic agents, food science, etc., can solve problems such as difficult to make innovative breakthroughs, separation and purification of active polysaccharides, and difficult structure identification, so as to reduce lipids quality accumulation, simple preparation and high yield

Inactive Publication Date: 2012-12-19
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The edible and medicinal mushroom Pleurotus eryngii has high nutritional value, and its active components such as polysaccharides have attracted more and more attention because of their excellent health care and therapeutic functions. However, most of the current research is limited to the optimization of polysaccharide extraction processes. A large number of low-level repetitive studies have been conducted, and there are relatively few studies on the separation of active ingredients and metabolic regulation mechanisms, so it is difficult to make innovative breakthroughs
Through in vitro biochemical experiments, it has been proved that the water-soluble polysaccharide (PEPE) of Pleurotus eryngii has a strong ability to scavenge free radicals, and it has been confirmed that PEPE can improve antioxidant enzymes by increasing the antioxidant activity in the carbon tetrachloride-induced liver injury mouse model. activity to protect the liver; at the same time, the results of animal models of hyperlipidemia show that it has the effect of lowering blood lipids, but the separation, purification and structural identification of active polysaccharides are still difficult problems

Method used

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  • Pleurotus eryngii polysaccharide capable of reducing accumulation of foam cell lipid and preparation method thereof
  • Pleurotus eryngii polysaccharide capable of reducing accumulation of foam cell lipid and preparation method thereof
  • Pleurotus eryngii polysaccharide capable of reducing accumulation of foam cell lipid and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment one, the preparation of polysaccharide

[0021] (1) Separation and purification of the active polysaccharides of Pleurotus eryngii was first carried out, and the dried Pleurotus eryngii fruiting mushroom powder was leached with hot water at 90°C for 3 hours, and the crude polysaccharide PE was obtained by four-fold volume ethanol precipitation;

[0022] (2) Crude polysaccharide PE passes through a dialysis membrane with a molecular cut-off of 3.5 kDa, dialyzes overnight with deionized water to remove small molecular weight impurities, redissolves in deionized water after drying the precipitate, adds trypsin to remove protein, and adds hydrogen peroxide to remove pigment , and finally add four times the volume of absolute ethanol to precipitate the polysaccharide, and obtain water-soluble Pleurotus eryngii polysaccharide (PEPE) after centrifugal drying;

[0023] (3) Separate and purify PEPE with DEAE-52 anion exchange column, use 0.05 M phosphate buffer (PBS) ...

Embodiment 2

[0025] Example 2: After derivatization of PEPE-1 and PEPE-2, two single polysaccharide components were detected by gas chromatography, and the monosaccharide composition analysis of PEPE-1 and PEPE-2 was carried out by complete acid hydrolysis (TFA hydrolysis) Post-gas chromatographic analysis method. The sample was hydrolyzed with 2 mol / L TFA 4 mL at 110°C for 4 h, the hydrolyzed sample solution was evaporated to dryness under reduced pressure, and then about 0.5 mL of methanol was added to the sample to dissolve the sample completely. ) were evaporated to dryness three times to obtain fully hydrolyzed monosaccharide components. The obtained sample acid hydrolyzate and standard monosaccharides were acetylated and then detected by gas phase. Finally, the composition and ratio of monosaccharides in the sample were estimated according to the retention time and peak area of ​​the standard monosaccharides. The results are shown in Table 1.

[0026] Table 1 Gas phase analysis res...

Embodiment 3

[0031] Example three: Fourier transform infrared spectrum detection of PEPE-1 and PEPE-2 two single polysaccharide components, the results are shown as image 3As shown, the infrared spectra of the two single polysaccharide components of PEPE-1 and PEPE-2 show that this group of high polymers has no derivatization groups, which are polyhydroxy compounds, and the polysaccharides are in the pyranose configuration. Periodic acid can selectively oxidize and break down the dihydroxyl or trihydroxyl groups in sugar molecules to generate corresponding polysaccharide aldehydes, formaldehyde or formic acid. The reaction proceeds quantitatively, and one molecule of periodic acid is consumed for every C-C bond split. The sugar group bonded at the 1→2 position is oxidized by periodic acid, and each sugar group consumes only one molecule of periodic acid on average, and no formic acid is released; the sugar group bonded at the 1→4 position is oxidized by periodic acid, On average, each s...

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Abstract

The invention discloses a pleurotus eryngii polysaccharide capable of reducing the accumulation of foam cell lipid and a preparation method of the pleurotus eryngii polysaccharide. The pleurotus eryngii polysaccharide is the pleurotus eryngii polysaccharide PEPE-1 of which the average molecular weight is 37.50kDa or the pleurotus eryngii polysaccharide PEPE-2 of which the average molecular weight is 30.00kDa; and the monosaccharide mainly consists of glucose, and a small quantity of mannose and galactose. The preparation method of the pleurotus eryngii polysaccharide comprises the following steps of: digesting and dialyzing pleurotus eryngii sporocarp dry powder by hot water, and further passing through an anion-exchange column DEAE-52 and separating and purifying by the salt concentration gradient elution to obtain PEPE-A; and further separating and purifying the PEPE-A by SephadexG-100, so that the components PEPE-1 and PEPE-2 can be obtained. The PEPE-1 and the PEPE-2 have a single peak, so that the pleurotus eryngii polysaccharide can preferably prevent the foam cell lipid accumulation function and the anti-oxygenation function; and the pleurotus eryngii polysaccharide can be used as a food additive.

Description

technical field [0001] The invention relates to the separation and purification of Pleurotus eryngii polysaccharide, in particular to a preparation method of Pleurotus eryngii polysaccharide capable of reducing foam cell lipid accumulation. Background technique [0002] In recent years, with the development of the economy and the improvement of the quality of life, diseases caused by unreasonable eating habits, such as obesity, hyperlipidemia, and atherosclerosis, have shown an increasing trend year by year, and a large number of research results have shown that if the Effective control of blood lipid levels can effectively prevent the occurrence of diseases. The edible and medicinal mushroom Pleurotus eryngii has high nutritional value, and its active components such as polysaccharides have attracted more and more attention because of their excellent health care and therapeutic functions. However, most of the current research is limited to the optimization of polysaccharide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61P39/06A23L1/09
Inventor 陆玲雍阳阳陈晶晶邢美春
Owner NANJING NORMAL UNIVERSITY
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