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Preparation method of grease with humanized structure

A technology of structured oil and breast milk, applied in dairy products, edible oil/fat, milk preparations, etc., can solve the problems of inappropriate content of palmitic acid at the Sn-2 position, complex production process, short reaction time, etc., and achieve enzyme duplication The effect of high number of times of use, wide range of applications, and short reaction time

Active Publication Date: 2012-11-14
ZANYU TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The invention provides a method for preparing breast-emulsified structured oil, which has good raw material safety, simple preparation process, short reaction time, less enzyme amount and low cost, and can be prepared to obtain the same composition as breast milk in terms of fatty acid composition and triglyceride structure distribution. The fat is extremely similar to breastmilk structure oil, which solves the problems of complex production process, high cost and inappropriate content of Sn-2 palmitic acid in the product in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Put 50g of palm oil with a palmitic acid content of 58.3% into a 250mL round bottom flask, place it in a water-bath shaker at 60°C, add 10g of non-specific lipase Novozym 435 (purchased from Novozymes, Denmark, enzyme Live is 10000PLU / g), reacted 5 hours with the rotating speed shaker of 250 rev / mins, filter and remove non-specific lipase, adopt gas chromatography-hydrogen flame ionization detector (GC-FID) to detect, Sn in the product -2 palmitic acid content is 58.7%.

[0043] (2) Triglyceride 50g and 125g mixed fatty acid (capric acid 3%, lauric acid 10%, myristic acid 4%, stearic acid 10%, oleic acid 50% triglyceride 50g and 125g with Sn-2 position palmitic acid content , linoleic acid 20%, linolenic acid 3%) were put into 250mL round bottom flask, placed in 60 ℃ water-bath shaker, added 17.5g 1, 3 specificity lipase Lipozyme RM IM (purchased from Denmark Novozymes company, the enzyme activity is 5-6BAUN / g), and reacted for 3 hours with a rotating speed shaker ...

Embodiment 2

[0046] (1) Put 20g of palm oil with a palmitic acid content of 58.3% into a 250mL round-bottomed flask, add 80mL of n-hexane, place it in a water bath shaker at 55°C, add 3g of non-specific lipase Novozym 435 (purchased from Denmark Nuo Weixin Company, enzyme activity is 10000PLU / g), with 250 rpm speed shaker reaction for 4 hours, filter to remove non-specific lipase, adopt gas chromatography-hydrogen flame ionization detector (GC-FID) to carry out Detection shows that the content of palmitic acid at Sn-2 position in the product is 58.1%.

[0047] (2) Triglyceride 10g and 30g mixed fatty acid (capric acid 3%, lauric acid 10%, myristic acid 4%, stearic acid 10%, oleic acid 50% triglyceride 10g and 30g mixed fatty acid with Sn-2 position palmitic acid content , linoleic acid 20%, linolenic acid 3%) are put into 250mL round bottom flask, add 160mL n-hexane, place in 55 ℃ water-bath shaker, add 6g 1,3 specificity lipase Lipozyme TL IM (purchased from Danish Novozymes company, enz...

Embodiment 3

[0050] (1) 100g of palm oil with a palmitic acid content of 58.3% is put into a 1L glass reactor, and after dehydration at 110°C for 1 hour under a vacuum of 0.08MPa, 1g of sodium ethylate is added, vacuumed at 90°C (air residual pressure lower than 0.06MPa) and stirred for 60 minutes, then cooled to 60°C, added 5mL of 10% citric acid to stop the reaction, stirred for 10 minutes and then washed with warm water at 30°C for 10 minutes to remove sodium ethoxide. Detected by gas chromatography-hydrogen flame ionization detector (GC-FID), the content of Sn-2 palmitic acid in the product is 59.1%.

[0051] (2) Triglyceride 50g and 100g mixed fatty acid (capric acid 3%, lauric acid 10%, myristic acid 4%, stearic acid 10%, oleic acid 50% triglyceride 50g with 59.1% content of Sn-2 position palmitic acid , 20% linoleic acid, 3% linolenic acid) into a 250mL round bottom flask, placed in a water bath shaker at 55°C, and added 15g of 1,3-specific lipase Lipozyme TL IM (purchased from Novo...

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PUM

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Abstract

The invention discloses a preparation method of grease with a humanized structure. The preparation method comprises the following steps: carrying out intra-molecular rearrangement reaction to palm oil with palmitic acid content of higher than 50% under the action of catalyst to obtain Sn-2 locus triglyceride with palmitic acid content of higher than 50%; and carrying out ester exchange reaction to the Sn-2 locus triglyceride with palmitic acid content of higher than 50% with mixed fatty acid or non-glyceride under the action of 1,3 locus Specificity lipase, and then subjecting the reaction product to after treatment, so as to obtain the grease with humanized structure, wherein the mixed fatty acid is mixture of at least two selected from the group consisting of decanoic acid, lauric acid,myristic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid and linolenic acid. The vegetable oils are used as the raw material according to the method, so the safety is high; the preparation process is simple, the reaction time is short, the enzyme dosage is low, and the cost is low; and the prepared grease with the humanized structure is very similar to the beast milk fat in the composition of fatty acid and the structural distribution of triglyceride, so the grease can be added in infant formula or formula foods and used as breast milk fat substitute.

Description

technical field [0001] The invention relates to the field of breastmilk fat substitutes, in particular to a method for preparing breastmilkized structured oil. Background technique [0002] Breast milk is the best food for human beings to provide to infants, which contains 3-5% triglycerides, and the main type of triglycerides is Sn-USU (U refers to unsaturated fatty acids, S refers to saturated fatty acids). Triglycerides are one of the main nutrients in breast milk, providing about 45% of the baby's energy and essential fatty acids. Breast milk is the best food for infant growth and development, but due to personal, cultural, socioeconomic and health reasons, more than 50% of families in my country use formula milk powder to feed their infants. The composition of fatty acids in breast milk will vary due to lactation stage, diet, season, race, geographical location, etc., but there are some things in common: in the total fatty acids, capric acid is 0.5-2%, lauric acid is 1...

Claims

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Application Information

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IPC IPC(8): C11C3/10A23C9/152A23D9/04
Inventor 冯凤琴冯永方陈锡威张辉韦伟
Owner ZANYU TECH GRP CO LTD
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