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Biomimetic specific immune adsorption material with PAMAM (Polyamidoamine) as spacer arm, and preparation method and application thereof

An immunoadsorbent material and biomimetic technology, which is applied in the field of preparation of biomimetic specific immunoadsorbent materials for blood purification, can solve the problems of difficult disinfection process, modification and shedding of proteins, and achieves improved clinical treatment effect, mild reaction conditions, The effect of improving adsorption performance

Active Publication Date: 2012-09-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, protein-based ligands have some defects that cannot be ignored: proteins are difficult to withstand the harsh disinfection process and are easy to fall off from the carrier, and these fallen-off ligands will cause safety problems; the high price of protein-based immunoadsorbent materials has caused great harm to patients. Heavy economic burden; protein ligands are difficult to modify with chemical functional groups, so they cannot be coupled to the carrier at the most suitable angle
The spacer arm commonly used in immunoadsorption materials is a linear spacer arm, and this type of spacer arm will entangle with biomacromolecules when separating biomacromolecules, thereby reducing the effect of immunoadsorption

Method used

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  • Biomimetic specific immune adsorption material with PAMAM (Polyamidoamine) as spacer arm, and preparation method and application thereof
  • Biomimetic specific immune adsorption material with PAMAM (Polyamidoamine) as spacer arm, and preparation method and application thereof
  • Biomimetic specific immune adsorption material with PAMAM (Polyamidoamine) as spacer arm, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Preparation of Azide Sepharose Gels

[0060] Add 4.0 g of drained agarose gel, 8 mL of epichlorohydrin and 4 mL of 3M sodium hydroxide solution into a 100 mL Erlenmeyer flask. After the mixture was stirred and reacted at room temperature for 3 h, it was washed with water and drained until the phenolphthalein did not turn red after adding 1.3M sodium thiosulfate to the washing liquid, and the epoxidized agarose was obtained. Dissolve 1.82g of sodium azide in 15mL of water, then add 4.0g of epoxidized agarose, and react at 20°C for 60h. After the reaction was completed, the gel was washed and dried to obtain an azide agarose gel. attached figure 1 The infrared spectrum shown is at 2100cm -1 The characteristic absorption peak of the azido group appeared at , which proved the existence of the azido group in the azide agarose.

[0061] Synthesis of G3.0PAMAM Dendrimer

[0062] In a 250ml round bottom flask, first add 200ml of methanol, then add 272mmol of methyl acrylat...

Embodiment 2

[0078] Preparation of Azide Sepharose Gels

[0079] Add 4.0 g of drained agarose gel, 8 mL of epichlorohydrin and 8 mL of 1.5 M sodium hydroxide solution into a 100 mL Erlenmeyer flask. After the mixture was stirred and reacted at room temperature for 4 h, it was washed with water and drained until the phenolphthalein did not turn red after adding 1.3M sodium thiosulfate to the washing liquid, and the epoxidized agarose was obtained. Dissolve 0.78g of sodium azide in 10mL of water, then add 4.0g of epoxidized agarose, and react at 40°C for 30h. After the reaction was completed, the gel was washed and dried to obtain an azide agarose gel.

[0080] Synthesis of G2.0PAMAM Dendrimer

[0081] In a 250ml round-bottomed flask, add 270ml of methanol first, then add 272mmol of methyl acrylate, place it in an ice-water bath for a period of time, so that the temperature of the system is about 10°C, and then continuously dropwise add 54.4mmol of propargylamine in 1 hour. 30ml of methan...

Embodiment 3

[0096] Preparation of Azide Sepharose Gels

[0097] Add 4.0 g of drained agarose gel, 8 mL of epichlorohydrin and 6 mL of 2.5 M sodium hydroxide solution into a 100 mL Erlenmeyer flask. After the mixture was stirred and reacted at room temperature for 3.5 h, it was washed with water and drained until the phenolphthalein did not turn red after adding 1.3M sodium thiosulfate to the washing liquid, thus obtaining epoxidized agarose. Dissolve 3.9g of sodium azide in 25mL of water, then add 4.0g of epoxidized agarose, and react at 60°C for 6h. After the reaction was completed, the gel was washed and dried to obtain an azide agarose gel.

[0098] Synthesis of G4.0PAMAM Dendrimer

[0099]In a 250ml round bottom flask, first add 180ml of methanol, then add 300mmol of methyl acrylate, place it in an ice-water bath for a period of time, so that the temperature of the system is about -10°C, and then continuously dropwise add 20.0mmol of alkyne within 1h. The methanol solution of propy...

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Abstract

The invention discloses a biomimetic specific immune adsorption material with PAMAM (Polyamidoamine) as a spacer arm, and a preparation method and the application thereof. The preparation process comprises the following steps of: firstly, obtaining sepharose gel azide by reaction of agarose epoxide and sodium azide; preparing a series of PAMAM dendrimer-like polymers in different generations by repeated addition reaction with methyl acrylate and amination reaction with ethidene diamine; after preparing amino acid methyl ester hydrochloride with the amino acid as the raw material, modifying the PAMAM dendrimer-like polymers with the amino acid methyl ester hydrochloride to prepare the amino acid modified PAMAM; coupling the amino acid modified PAMAM with sepharose gel azide through a click reaction so as to obtain the biomimetic specific immune adsorption material with PAMAM as the spacer arm. The material disclosed in the invention is prepared by simple synthetic steps, and the preparation is safe; products have the characteristics of strong specificity, high adsorbing efficiency to the immunoglobulin and excellent regeneration performance, and can be used for IgG (immunoglobulin G) separation and clinical immune adsorption treatment.

Description

technical field [0001] The invention belongs to biomedical devices, and in particular provides a method for preparing a biomimetic specific immunoadsorption material for blood purification. The adsorption material uses polyamide-amine dendritic polymer (PAMAM) as a spacer arm. Background technique [0002] It is well known that protein immunoadsorbent materials with protein A, G, etc. as ligands have good biospecific affinity to immunoglobulin (IgG), and the interaction between protein ligands and IgG involves hydrophobic and electrostatic interactions. Immunoadsorbent materials containing such biospecific ligands have been proven to remove autoantibodies to purify antibodies and treat various autoimmune diseases, and thus have been widely used in biological purification and clinical practice. However, protein-based ligands have some defects that cannot be ignored: proteins are difficult to withstand the harsh disinfection process and are easy to fall off from the carrier, a...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/30B01D15/08C07K16/00C07K1/22
Inventor 李光吉胡小艳
Owner SOUTH CHINA UNIV OF TECH
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