Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for measuring vitamins A, D and E in compound vitamin

A technology for complex vitamins and vitamins, applied in measuring devices, color/spectral characteristic measurements, instruments, etc., can solve the problems of low accuracy, cumbersome operation, and inability to detect vitamin A at the same time

Inactive Publication Date: 2011-11-23
TIANJIN TIANSHI BIOLOGICAL DEV +2
View PDF6 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the saponification method to process the multivitamin samples will lead to serious loss of vitamins A, D, and E in the multivitamins, which will cause the test results to not truly reflect the vitamin content in the sample and affect the accuracy of the test data.
[0006] 3. Poor reproducibility and low accuracy;
[0007] 4. Reagent consumption is large
[0008] Due to the different polarities of vitamins A, D, and E, vitamins A, D, and E cannot be detected simultaneously by existing technologies. Usually, vitamins A and E are separated by reversed-phase chromatography, detected by an ultraviolet detector, and internal standard Quantitative determination of vitamin A and vitamin E by method, vitamin D is separated by normal phase chromatographic system and then detected by reversed phase chromatographic system
The disadvantages of this detection method are: the life of the normal phase chromatographic column is short, the price is expensive, and it is cumbersome to use the two chromatographic systems of normal phase and reverse phase alternately, which increases the loss of samples and reduces the detection accuracy.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring vitamins A, D and E in compound vitamin
  • Method for measuring vitamins A, D and E in compound vitamin
  • Method for measuring vitamins A, D and E in compound vitamin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A method for measuring vitamin A, vitamin D and vitamin E in compound vitamins, comprising the steps of:

[0078] (1) Preparation of sample solution to be tested: take by weighing the multivitamin sample to be tested in m grams in a 100ml volumetric flask, add ammonia water to adjust to pH 8.0 (also can be controlled at 7.8 or 8.5), add 400mg (also It can be 300mg or 500mg) of trypsin, treated in an ultrasonic water bath at 65°C for 30min (or 20min or 40min), let it stand to cool to room temperature, set the volume to the mark with ethanol, shake well and filter, filter out the residue, take V 1 Put the filtrate in a 100ml volumetric flask filled with 50ml petroleum ether and 5ml (or 10ml) water, vibrate the volumetric flask to separate the filtrate, and let it stand for 10min; take V 2 1 ml of petroleum ether extract was concentrated in a 40°C water bath under a nitrogen blowing device, and then dissolved in 1 ml of methanol to obtain the sample solution to be tested; ...

Embodiment 2

[0138] With the method of embodiment 1 adopt following condition to certain company's simultaneously containing vitamin A, vitamin D 2 , Vitamin D 3 The product samples were tested and found to be qualified.

[0139] Chromatographic conditions Chromatographic column Waters Symmetry C18 (250×4.6mm, 5μm); mobile phase acetonitrile-methanol-water system, ratio 25:75:4, flow rate 1.0ml / min, column temperature: 30°C, injection volume 50μl, The detection wavelength of vitamin D is 265nm. The detection wavelength of vitamin A is 300nm. Under these chromatographic conditions, vitamin D 2 , Vitamin D 3 Retention times of 22.917min, 22.605min ( Picture 1-1 ); The retention time of vitamin A is 9.130min ( Figure 1-2 ); Each substance is well separated, and the peak area of ​​each substance is plotted against the concentration to calculate the regression equation and the correlation coefficient. The correlation coefficient r is all > 0.999, indicating that the peak area has a good ...

Embodiment 3

[0145] Use the method of Example 1 to adopt the following conditions to contain vitamin D at the same time for a certain company 3 , α-tocopherol, γ-tocopherol, and δ-tocopherol product samples were tested, and their content was found to be qualified.

[0146] Chromatographic conditions Chromatographic column Waters Symmetry C18 (250×4.6mm, 5μm); mobile phase acetonitrile-methanol-water system, ratio 25:75:4, flow rate 1.0ml / min, column temperature: 30°C, injection volume 50μl, The detection wavelength of vitamin D is 265nm. The detection wavelength of vitamin E is 300nm. Under this chromatographic condition, the retention time of vitamin D3 is 23.759min ( Figure 2-1 ); the retention times of α-tocopherol, γ-tocopherol, and δ-tocopherol were 30.700min, 26.022min, 21.364min ( Figure 2-2). Each substance is well separated, and the peak area of ​​each substance is plotted against the concentration to calculate the regression equation and correlation coefficient. The correla...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for measuring vitamins A, D and E in a compound vitamin. The method comprises the following steps: (1) preparing solution of a sample to be measured; (2) preparing standard substance solution; (3) taking the solution of the sample to be measured, separating the solution prepared in the step (2) with a Waters reversed phase C18 chromatographic column and detecting the solution with a diode array detector to obtain a corresponding chromatogram and qualitatively deciding various vitamins in the solution of the sample to be measured according to the retention time of various vitamins in the standard substance solution; (4) respectively obtaining the mass concentrations of various vitamins in the solution of the sample to be measured via the proportional relationships of the peak areas of various vitamins in the chromatogram according to the concentrations of various vitamins in the standard substance solution; and (5) computing the contents of the vitamins in the solution of the sample to be measured through a formula according to the concentrations. The method has the following advantages: the method has the characteristic of simple, convenient, rapid and accurate measurement; and the analysis time is shortened and the organic reagent consumption is reduced while control of the raw materials of the health food is implemented.

Description

technical field [0001] The invention relates to a method for determining vitamins A, D and E in multivitamins of health food. Background technique [0002] Vitamins are essential nutrients to maintain the normal physiology of humans and animals. Although humans and animals have little demand for vitamins, their functions are very important. Because people cannot synthesize vitamins, they must rely on food to provide enough vitamins. With the development of society, more and more vitamin-containing products appear on the market, and more and more health products are labeled as rich in various vitamins. However, how many vitamins are contained in a certain vitamin-containing product, the method of determining vitamins in food is very important. [0003] In recent years, with the development of society, there are more and more products containing multivitamins, and the dosage forms include tablets or granules. Most of the vitamins in food are added in the form of multivitamins...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/60G01N21/33
Inventor 赵晓娟李星芝袁海新贾鑫
Owner TIANJIN TIANSHI BIOLOGICAL DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products