Method for measuring vitamins A, D and E in compound vitamin
A technology for complex vitamins and vitamins, applied in measuring devices, color/spectral characteristic measurements, instruments, etc., can solve the problems of low accuracy, cumbersome operation, and inability to detect vitamin A at the same time
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Embodiment 1
[0077] A method for measuring vitamin A, vitamin D and vitamin E in compound vitamins, comprising the steps of:
[0078] (1) Preparation of sample solution to be tested: take by weighing the multivitamin sample to be tested in m grams in a 100ml volumetric flask, add ammonia water to adjust to pH 8.0 (also can be controlled at 7.8 or 8.5), add 400mg (also It can be 300mg or 500mg) of trypsin, treated in an ultrasonic water bath at 65°C for 30min (or 20min or 40min), let it stand to cool to room temperature, set the volume to the mark with ethanol, shake well and filter, filter out the residue, take V 1 Put the filtrate in a 100ml volumetric flask filled with 50ml petroleum ether and 5ml (or 10ml) water, vibrate the volumetric flask to separate the filtrate, and let it stand for 10min; take V 2 1 ml of petroleum ether extract was concentrated in a 40°C water bath under a nitrogen blowing device, and then dissolved in 1 ml of methanol to obtain the sample solution to be tested; ...
Embodiment 2
[0138] With the method of embodiment 1 adopt following condition to certain company's simultaneously containing vitamin A, vitamin D 2 , Vitamin D 3 The product samples were tested and found to be qualified.
[0139] Chromatographic conditions Chromatographic column Waters Symmetry C18 (250×4.6mm, 5μm); mobile phase acetonitrile-methanol-water system, ratio 25:75:4, flow rate 1.0ml / min, column temperature: 30°C, injection volume 50μl, The detection wavelength of vitamin D is 265nm. The detection wavelength of vitamin A is 300nm. Under these chromatographic conditions, vitamin D 2 , Vitamin D 3 Retention times of 22.917min, 22.605min ( Picture 1-1 ); The retention time of vitamin A is 9.130min ( Figure 1-2 ); Each substance is well separated, and the peak area of each substance is plotted against the concentration to calculate the regression equation and the correlation coefficient. The correlation coefficient r is all > 0.999, indicating that the peak area has a good ...
Embodiment 3
[0145] Use the method of Example 1 to adopt the following conditions to contain vitamin D at the same time for a certain company 3 , α-tocopherol, γ-tocopherol, and δ-tocopherol product samples were tested, and their content was found to be qualified.
[0146] Chromatographic conditions Chromatographic column Waters Symmetry C18 (250×4.6mm, 5μm); mobile phase acetonitrile-methanol-water system, ratio 25:75:4, flow rate 1.0ml / min, column temperature: 30°C, injection volume 50μl, The detection wavelength of vitamin D is 265nm. The detection wavelength of vitamin E is 300nm. Under this chromatographic condition, the retention time of vitamin D3 is 23.759min ( Figure 2-1 ); the retention times of α-tocopherol, γ-tocopherol, and δ-tocopherol were 30.700min, 26.022min, 21.364min ( Figure 2-2). Each substance is well separated, and the peak area of each substance is plotted against the concentration to calculate the regression equation and correlation coefficient. The correla...
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