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Method for improving vitamin D content in cordyceps sinensis calcium strengthening agent by ultraviolet irradiation

A technology of Cordyceps sinensis calcium and fortifier, which is applied in the directions of botanical equipment and methods, medical preparations containing active ingredients, and pharmaceutical formulas, etc. question

Inactive Publication Date: 2013-03-20
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although edible and medicinal fungi are an important source of vitamin D in natural foods, their content is far from meeting the human body's demand for vitamin D
Vitamin D in calcium fortifiers currently on the market is also artificially added to assist calcium absorption, which does not have bioidentity and balance

Method used

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  • Method for improving vitamin D content in cordyceps sinensis calcium strengthening agent by ultraviolet irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] The strain used: the strain of Cordyceps sinensis of CCTCC AF99009.

[0081] A, prepare liquid culture medium, add following composition (weight / volume percentage) in every 100ml culture medium:

[0082] Sucrose 3.0g

[0083] Corn flour 3.0g

[0084] Soybean powder 1.5g

[0085] Yeast powder 0.1g

[0086] K H 2 PO 4 0.05g

[0087] MgSO 4 0.02g

[0088] FeSO 4 Add iron 0.01g

[0089] ZnSO 4 Add zinc 0.01g

[0090] Take Na 2 SeO 3 Added Selenium 0.00002g

[0091] to CrCl 3 Add chromium 0.00002g

[0092] CaCO 3 Add calcium 0.2g

[0093] VB 1 0.002g

[0094] Add water to dissolve and make the volume to 100ml;

[0095] Sterilize at 120°C for 30 minutes and then cool to 30°C;

[0096] b. Inoculate the medium of the above step a into the culture medium of the above-mentioned step a and inoculate the Cordyceps sinensis fungus (Cordyceps sinensis) with the preservation number CCTCC AF99009 in the Chines...

Embodiment 2

[0126] Example 2: The vitamin D content in the product reaches 35mg / 100g

[0127] The strain used: the strain of Cordyceps sinensis of CCTCC AF99009.

[0128] A, prepare liquid culture medium: add following composition (weight / volume percentage) in every 100ml culture medium:

[0129] Sucrose 4.0g

[0130] Corn flour 4.0g

[0131] Soybean powder 2.0g

[0132] Yeast powder 0.2g

[0133] K H 2 PO 4 0.1g

[0134] MgSO 4 0.02g

[0135] FeSO 4 Add iron 0.01g

[0136] ZnSO 4 Add zinc 0.01g

[0137] Take Na 2 SeO 3 Added Selenium 0.00002g

[0138] to CrCl 3 Add chromium 0.00002g

[0139] with CaCl 2 Add calcium 0.3g

[0140] VB 1 0.003g

[0141] Add water to dissolve and make the volume to 100ml;

[0142] Sterilize at 120°C for 30 minutes and then cool to 30°C;

[0143] b. Inoculate the medium of the above step a into the culture medium of the above step a and inoculate the Cordyceps sinensis fungu...

Embodiment 3

[0174] The strain used: the strain of Cordyceps sinensis of CCTCC AF99009.

[0175] A, prepare liquid culture medium: add following composition (weight / volume percentage) in every 100ml culture medium:

[0176]Sucrose 6.0g

[0177] Corn flour 4.0g

[0178] Soybean powder 2.0g

[0179] Yeast powder 0.3g

[0180] K H 2 PO 4 0.1g

[0181] MgSO 4 0.02g

[0182] FeSO 4 Add iron 0.01g

[0183] ZnSO 4 Add zinc 0.01g

[0184] Take Na 2 SeO 3 Added Selenium 0.00002g

[0185] to CrCl 3 Add chromium 0.00002g

[0186] With Ca(NO 3 ) 2 Added Calcium 0.4g

[0187] VB 1 0.003g

[0188] Add water to dissolve and make the volume to 100ml;

[0189] Sterilize at 120°C for 30 minutes and then cool to 30°C;

[0190] b. Inoculate the medium of the above step a into the culture medium of the above-mentioned step a and inoculate the Cordyceps sinensis fungus (Cordyceps sinensis) with the preservation number CCTCC AF99009 prese...

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Abstract

The invention discloses a method for improving vitamin D content in a cordyceps sinensis calcium strengthening agent by ultraviolet irradiation, which comprises the following steps of: a, preparing a culture medium: preparing a liquid culture medium from a carbon source, a nitrogen source, mineral elements and the like, and sterilizing the prepared culture medium; b, inoculating cordyceps sinensis fungus into the culture medium, and performing shake culture to prepare liquid cordyceps sinensis fungus; c, inoculating the cordyceps sinensis fungus into the culture medium, performing the shake culture under certain conditions, and taking fermentation liquor out; and d, selecting a UV-B ultraviolet light source with the power of 14 to 60 W and the wavelength of 280 to 325 nm, pouring the cordyceps sinensis fungus fermentation liquor into a large culture dish, placing the ultraviolet light source over the culture dish, irradiating the prepared fermentation liquor, concentrating and drying the fermentation liquor, and breaking wall and grinding the obtained product into a powdery product. The method has the advantages of simple process, convenient operation and wide application to prevention and treatment of rickets of children, acalcerosis of women and osteoporosis of old peoples.

Description

technical field [0001] The present invention relates to calcium-supplementing food and pharmaceutical products, and more specifically relates to a method for increasing the vitamin D content in Cordyceps sinensis calcium fortifier by ultraviolet light irradiation. Alcohol Photoconversion to Vitamin D 2 It can be used in the preparation of medicines or health food for the prevention and treatment of calcium deficiency in children and women; the osteoporosis of the elderly and other diseases. Background technique [0002] A Chinese patent discloses a patent on "The preparation method of Cordyceps sinensis bioactive multidimensional enhancer" (application number is 03106294.6). The medicinal fungus Cordyceps sinensis is used as a biological carrier, and inorganic calcium can be converted into active calcium in a chelated state of biological macromolecules through liquid fermentation. The product contains a variety of vitamins, bacterial immune proteins and bacterial polysacch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/29A61K36/068A01G1/04A23L33/00
Inventor 胡征
Owner HUBEI UNIV OF TECH
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