Azoxystrobin compound sterilization composition
A composition, azoxystrobin technology, applied in the directions of fungicides, biocides, biocides, etc., to achieve the effects of reducing the dosage of field drugs, delaying disease resistance, and improving control effects
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example 1
[0017] Bioassay Example 1: Toxicity determination of azoxystrobin and prochloraz compounded against blast fungus
[0018] Test object: Magnaporthe grisea
[0019] The test refers to the "Indoor Bioassay Test Guidelines for Pesticides NY / T1156.2-2006" This test adopts the living pot test method, 2 rice plants are planted in each pot, and each concentration is replicated five times, with a total of 10 rice plants in each treatment. Water treatment was used as blank control. Wash the surface spores of the cultivated pathogenic bacteria with sterile water to prepare a concentration of 1×10 6 The spore suspension of 4 spores / ml was evenly sprayed and inoculated on the rice leaves with a crop sprayer when the rice was at the three-leaf one-core stage. After the treatment, the rice was bagged, sprayed 24 hours later, placed in an artificial climate chamber to moisturize, and taken out after 7 days. Count the number of diseased spots on the first, second and third leaves (30 leaves...
example 2
[0024] Bioassay Example 2: Indoor Toxicity Determination of Azoxystrobin and Prochloraz against Cucumber Powdery Mildew
[0025] Test object: Cucumber powdery mildew pathogen
[0026] The test refers to "Pesticide Bioassay Technology" (Edited by Chen Nianchun, published by Beijing Agricultural University Press), and "Pesticide Indoor Bioassay Test Guidelines NY / T1156.2-2006" This test uses the cucumber powdery mildew pot test method. On the basis of the preliminary test, select 2 melon seedlings at the true leaf stage with the same growth, write the label number with an oily marker, insert it into the pot of cucumber seedlings, discharge them in sequence, and spray the medicine on the melons by spraying the leaves. On the leaves of seedlings, after spraying treatment, dry the test materials in the fume hood or greenhouse for 24 hours before inoculation. Cultivate in the greenhouse for 7 days, investigate the control effect, the grading standard implements the national standar...
example 3
[0031] Bioassay Example 3: Indoor Toxicity Determination of Azoxystrobin and Prochloraz to Cucumber Botrytis Botrytis
[0032] Test object: Cucumber Botrytis cinerea
[0033] The test refers to "Pesticide Bioassay Technology" (Edited by Chen Nianchun, published by Beijing Agricultural University Press), and "Pesticide Indoor Bioassay Test Guidelines NY / T1156.2-2006" This test uses the growth rate method (plate method). On the basis of the preliminary test, on the aseptic operation table, quantitatively add the pre-thawed sterilized medium into the sterile Erlenmeyer flask according to the test treatment. Have 45ml of heat medium (PDA medium, 45-50 ℃) in the Erlenmeyer flask, after shaking well, pour into the glass petri dish of diameter 90mm rapidly, each petri dish is poured into 10ml of culture medium with medicine. Set aside horizontally to cool and form into slabs. Each concentration was replicated five times. The treatment without the active ingredient of the drug was ...
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