Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Total triterpene of garden burnet root with anti-inflammatory and analgesic effects and preparation method thereof

An anti-inflammatory, analgesic, and total triterpene technology, which is applied in the field of medicine, can solve problems such as the preparation method of total triterpenes of Burnet burnet, and achieve the effect of simple preparation process and clear mechanism of action

Inactive Publication Date: 2009-12-02
SHENYANG PHARMA UNIVERSITY
View PDF0 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the preparation method of the total triterpenes of Burnet burnet and its application in medicines for treating proctitis, ulcerative colitis, pyelonephritis, burns and scalds, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Total triterpene of garden burnet root with anti-inflammatory and analgesic effects and preparation method thereof
  • Total triterpene of garden burnet root with anti-inflammatory and analgesic effects and preparation method thereof
  • Total triterpene of garden burnet root with anti-inflammatory and analgesic effects and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 5kg of Burnet coarse powder, add 35L of 70% ethanol to soak overnight, heat and reflux extraction twice, the extraction time is 2 hours and 2 hours respectively, combine the alcohol extracts, filter, and concentrate under reduced pressure at 60°C to reach the crude drug content in the solution. The concentration is 30g / ml, filter; the filtrate is adsorbed by D101 macroporous adsorption resin, the amount of resin is 6kg, the impurities are washed away with water and 20% ethanol respectively, and then eluted with 75% ethanol, the alcohol eluate is collected, and depressurized The solvent was recovered, the obtained crude product was suspended in an aqueous solution, and then subjected to polyamide column chromatography, washed with water first, and then fully eluted with 75% ethanol, and the ethanol was recovered under reduced pressure to obtain 340 g of the total triterpenes.

Embodiment 2

[0020] Take Burnet burnet coarse powder 5kg, add 45L 70% ethanol to soak overnight, heat and reflux extraction 3 times, the extraction time is 2, 2, 1 hour respectively, combine the extracts, filter, and concentrate under reduced pressure at 60°C until the crude drug contained in the solution The concentration is 40g / ml, filter; the filtrate is adsorbed by D101 macroporous adsorption resin, the amount of resin is 6kg, the impurities are washed away with water and 20% ethanol respectively, and then eluted with 70% ethanol solution, the ethanol eluate is collected, decompressed The solvent was recovered, the obtained crude product was suspended in an aqueous solution, and then extracted with equal amounts of ethyl acetate and n-butanol respectively, each extracted 4 times, the solvent was recovered under reduced pressure, and the extracts were combined to obtain 310 g of the total triterpenes.

Embodiment 3

[0021] Example 3 Inhibitory effect of total triterpenes of Burnet burnet on mouse ear swelling induced by xylene

[0022] (1) Experimental materials

[0023] Animals: male KM mice (25±2g), fasted for 24 hours before administration, free to drink water.

[0024] Reagents: normal saline, xylene, 0.5% CMCNa, aspirin (Shenyang Wanrong Pharmaceutical Co., Ltd., product batch number: 061205), indomethacin (Indomethacin) (Shanxi Yunpeng Pharmaceutical Co., Ltd., product batch number: A070801 ).

[0025] Materials and instruments: 1ml mouse gavage device, 1ml syringe, surgical scissors, tweezers, hole punch, volumetric flask, electronic balance (CPJ603 Ohaus Instruments (Shanghai) Co., Ltd.).

[0026] (2) Experimental method

[0027] Mice were randomly divided into 5 groups, 10 in every group: negative control group (0.2% CMCNa, 10ml / kg, i.g.), aspirin control group (30mg / kg -1 , i.g,), indomethacin control group (10mg / kg -1 , i.g.), drug group (100, 30mg / kg, i.g.). Thirty minut...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicaments, and provides a method for preparing total triterpene of Chinese medicament garden burnet root. The total triterpene is mainly obtained by performing reflux extraction by 70 percent ethanol, absorbing by a macroporous absorbent resin, using water to wash off impurities, using ethanol solution for elution, then decolorizing an eluent by daiamid powder, using the ethanol solution for elution, and recovering the ethanol through pressure reduction. The total triterpene mainly contains triterpenic acid active components such as arjunic acid, 1beta-euscaphic acid, euscaphic acid, tormentic acid and the like, and triterpenoid saponin active components such as sanguisorbin I, sanguisorbin II, sanguisorbin III, niga-ichigoside, kaji-ichigoside F1 and the like. Experimental results show that the total triterpene has remarkable anti-inflammatory and analgesic effects. The total triterpene has definite action substance basis and action mechanism as well as simple preparation technology, is suitable for industrialized production, can be taken as medicinal raw materials for treating inflammatory diseases such as burn and scald, rectitis, ulcerative colitis, pyelonephritis and the like, and is prepared into clinically acceptable dosage forms such as tablets, capsules, granules or compound preparations according to the prior process.

Description

technical field [0001] The invention relates to the technical field of medicines, and relates to a preparation method of total triterpenes of Burnet eucalyptus, and also relates to a medical application of total triterpenes of Burnet eucalyptus in treating proctitis, ulcerative colitis, pyelonephritis, burns and scalds and other inflammatory diseases. Background technique [0002] Sanguisorba officinalis L. has the effect of cooling blood to stop bleeding, detoxifying and astringing sores. It is widely used and has definite curative effect. It is widely used clinically in the treatment of proctitis, ulcerative colitis, pyelonephritis, burns and other inflammatory diseases, as well as internal and surgical hemostasis. The research on the chemical constituents of Burnet root shows that: Burnet is rich in triterpene acids and triterpenoid saponins, and mainly ursane-type and oleanane-type triterpenes. [0003] Previous literature has reported that the water and alcohol extract...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/739A61P29/00A61P1/00A61P1/04A61P13/12A61P17/02A61K125/00
Inventor 原忠王寒罗艳谷雪
Owner SHENYANG PHARMA UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products