Culture fluid for promoting formation of tissue engineering skin lipid and formulating method thereof
A tissue-engineered skin and culture fluid technology is applied to the culture fluid and its preparation field for promoting the formation of tissue-engineered skin lipid structures, and can solve the problems of high misjudgment rate, cumbersome operation during use, inconsistent data, etc. The ability of structure formation, the simple preparation method and the effect of strong stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment Construction
[0043] For further illustrating the present invention, specifically set forth in conjunction with the following examples:
[0044] 1. Preparation of culture medium:
[0045] Its components and the consumption of each component are:
[0046] (1) Basal culture medium: mixed with the following substances in volume percentage:
[0047] DMEM culture medium 65%
[0048] F12 medium 25%
[0049] Fetal Bovine Serum 10%
[0050] (2) Nutrients:
[0051] Hydrocortisone 0.4~0.5μg / mL
[0052] Epidermal growth factor 5~15ng / mL
[0053] Isoproterenol 1~3×10 -6 mol / L
[0054]Insulin 5~15μg / mL
[0055] Transferrin 5~10μg / mL
[0056] Triiodothyronine 2~6×10 -9 mol / L
[0057] Adenine 1~3×10 -4 mol / L
[0058] Vitamin C 50~100μg / mL
[0059] Retinoic acid 4~10μg / mL
[0060] Cholera toxin 0.1~1nmol / L
[0061] L-serine 1~3×10 -2 mol / L
[0062] L-carnitine 1~5×10 -5 mol / L
[0063] Palmitic acid 15~35μmol / L
[0064] Linoleic acid 5~25μmol / L
[0065] Arachidonic acid 5~10μmol / L
...
PUM
Property | Measurement | Unit |
---|---|---|
thickness | aaaaa | aaaaa |
thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com