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Silk-screen printing electrode and method for synthesizing chloramphenicol molecular imprinting film

A technology of screen printing electrodes and molecularly imprinted membranes, which is applied in the field of polymers, can solve the problems of not being able to replace solid phase enrichment, separation devices, extraction cartridges, high cost, and long preparation time, and achieve favorable detection results and processing The method is simple and the holes are evenly distributed

Inactive Publication Date: 2009-03-18
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the template molecules bound to the molecularly imprinted microparticles in the extraction column are difficult to elute by conventional chemical methods, so the concentration of the test substance obtained through the extraction column is too low, which greatly affects the detection sensitivity. However, it cannot replace the solid-phase enrichment and separation devices used in existing high-performance liquid chromatography analysis instruments.
The extraction cartridge has high cost, long preparation time, and it is difficult to connect with automatic detection equipment, making it less valuable in practical applications

Method used

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  • Silk-screen printing electrode and method for synthesizing chloramphenicol molecular imprinting film
  • Silk-screen printing electrode and method for synthesizing chloramphenicol molecular imprinting film
  • Silk-screen printing electrode and method for synthesizing chloramphenicol molecular imprinting film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Use screen printing technology to print conductive silver paste on the electrode substrate 1 to print two electrode connecting wires 3 and the substrate of the connecting terminal 2, the surface of the electrode connecting wire 3 is coated with a layer of PVC insulator, and the working electrode 4 and the counter electrode 5 at the lower end A layer of conductive graphite is coated by sputtering graphite method. Soak in 50% nitric acid, and then rinse with distilled water for 3 times. Let dry and store at 4°C for later use.

[0048] Weigh 0.05g of chloramphenicol and 70μl of methacrylic acid functional monomer and dissolve in 380μl of tetrahydrofuran solvent, mix ultrasonically for 10 minutes, add ethylene glycol dimethacrylic acid crosslinking agent, mix ultrasonically for 10 minutes, and then add to the reaction system 70 μl of water-based polyurethane adhesive, after ultrasonic mixing for 10 minutes, add 0.02 g of initiator azoisobutyrocyanide, after ultrasonic mixi...

Embodiment 2

[0056] During the preparation of chloramphenicol molecularly imprinted membranes, the ultraviolet polymerization time was continuous irradiation for 30 minutes, and other conditions remained unchanged. During the detection process, the preparation of the standard curve was the same as in Example 1, and pork was used as the detection sample.

[0057] Sample processing: Take 5 pork samples confirmed by high performance liquid chromatography-mass spectrometry to not contain chloramphenicol residues, take 1 gram of meat samples, put them into the sample cup of the tissue homogenizer, add 10 ml of 0.1M pH to 7.2 Phosphate buffer solution, crushed and homogenized, transferred to a 15ml centrifuge tube, added 1ml of chloramphenicol standard solution containing 40μg / L, then extracted with 6ml of ethyl acetate, shaken and mixed for 15min, Collect the organic phase containing chloramphenicol, evaporate and remove the organic solvent at 40°C and 240mbar, and finally add acetonitrile-2M p...

Embodiment 3

[0059] In the preparation process of the chloramphenicol molecularly imprinted membrane, the screen-printed electrode terminals, electrode connections, working electrodes and counter electrodes were all printed with conductive silver paste, and the others were the same as in Example 1. During the detection process, the preparation of the standard curve was the same as in Example 1, and chicken eggs were used as the detection sample.

[0060] Sample treatment: Take 5 egg samples confirmed by high performance liquid chromatography-mass spectrometry that do not contain chloramphenicol residues, take 5ml and put them in a 10ml centrifuge tube, shake and mix for 15min, add 1ml of chloramphenicol containing 20μg / L Standard solution, then extracted with 3ml ethyl acetate, collected the organic phase containing chloramphenicol, evaporated to remove the organic solvent at 40°C and 240mbar, and finally added 5ml acetonitrile-2M perchloric acid aqueous solution, the volume ratio was 3:2, ...

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Abstract

The invention relates to a silk screen printing electrode and a method for synthesizing chloramphenicol molecularly imprinted membrane, belonging to the macromolecule technical field. The invention embeds template molecules in the direct surface in-situ polymerization of a one-time silk screen printing electrode and elutes the template molecules, to form the cavities of fixed sizes and shapes and the function groups of a fixed arrangement in the molecularly imprinted membrane, therefore, the molecularly imprinted membrane has shape memory property to the stereo structure of the template molecules, the chloramphenicol molecule of the object sample can be combined with the cavity specifically, to modify the electromechanical property of the molecularly imprinted membrane to improve the electric conductivity of the molecularly imprinted membrane and improve membrane passing current. The silk screen printing electrode coated with a chloramphenicol molecularly imprinted membrane can be connected with a current sensor to quickly and sensitively detect chloramphenicol density. The molecularly imprinted membrane can be used to detect chloramphenicol, having simple sample pretreatment, short detection time, strong interference resistance and batch production.

Description

technical field [0001] The invention relates to an electrode in the technical field of polymers and a synthesis method thereof, in particular to a screen printing electrode and a method for synthesizing a chloramphenicol molecularly imprinted membrane. Background technique [0002] As a common high-efficiency broad-spectrum antibacterial drug, chloramphenicol is used illegally in animal husbandry and aquaculture, such as the use of chloramphenicol as an antibacterial drug to control typhoid and other Salmonella infections. With the improvement of people's living standards, people's awareness of health and environmental protection has gradually increased, consumers' requirements for food quality have increased, and they have paid more attention to the nutrition and safety of food. Therefore, food safety issues have been paid more and more attention in my country. Research at home and abroad shows that, Residual chloramphenicol inhibits the hematopoietic function of the bone ma...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N30/56
Inventor 柴春彦刘国艳张挪威李锋刘海峰
Owner SHANGHAI JIAO TONG UNIV
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