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Enteric oral vaccine for fishing gear and preparation method thereof

An oral vaccine and enteric-coated technology, which is applied in the field of fish oral vaccines, can solve the problems of destroying the immunogenicity of vaccines, lack of particles, and the inability to establish vaccine controlled release technology, etc., to efficiently stimulate immune responses, enhance adhesion firmness, and improve The effect of immune efficiency

Inactive Publication Date: 2008-10-15
天津市水产研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above technologies have not carried out research on the physiological characteristics of the gastrointestinal tract of fish, nor have they carried out enteric test observations, and cannot establish a suitable vaccine controlled release technology. Identify the release site of microparticle and microcapsule vaccines in the gastrointestinal tract
The materials used in its preparation technology do not have a definite pH dependence, can only achieve oral delivery and exert a certain sustained release effect, and cannot effectively achieve the purpose of intestinal release. If the vaccine is released too early, it will be digested by gastric acid and digestive enzymes , destroy, and cannot effectively protect the vaccine from acting on the immune-related lymphoid tissue of the intestinal mucosa
Moreover, in the preparation process of the above technology, the vaccine needs to be miscible into the organic solvent of the polymer material. The existence of the organic solvent will destroy the immunogenicity of the vaccine, resulting in unstable immune effect of the vaccine, which further limits its application in production.
[0005] Due to the influence of gastrointestinal factors, most protein drugs are easily inactivated and poorly absorbed, resulting in poor bioavailability, and their oral drug delivery system still faces challenges

Method used

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  • Enteric oral vaccine for fishing gear and preparation method thereof
  • Enteric oral vaccine for fishing gear and preparation method thereof
  • Enteric oral vaccine for fishing gear and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation method: select 10-30 mesh starch granules and set aside.

[0028] The first step of vaccine spraying: the dry powder composition A is dissolved in water, and it is formulated into an aqueous suspension with a solid content of 5%, and then an appropriate amount of flounder ascites disease vaccine physiological saline solution (10 8 CFU / ml), prepared into 10 6 The suspension at the concentration of CFU / ml vaccine is evenly sprayed on the surface of the particles using a high-pressure spray gun. Vaccine spray amount 10 6 CFU / g.

[0029] The second step of coating the isolation layer: the dry powder composition A was dissolved in water to prepare a suspension with a solid content of 5%, and evenly sprayed on the surface of the vaccine layer with a high-pressure spray gun. The coating weight gain was 2%.

[0030] Dry powder composition A formula consists of:

[0031] Hypromellose E5 25g

[0032] Hypromellose E15 22g

[0033] Polyvinyl alcohol S205 13g

[...

Embodiment 2

[0070] Preparation method: select 10-30 mesh starch granules and set aside.

[0071] The first step of vaccine spraying: the dry powder composition A is dissolved in water, configured into an aqueous suspension with a solid content of 8%, and then an appropriate amount of flounder ascites disease vaccine physiological saline solution (10 10 CFU / ml), prepared into 10 7 The suspension at the concentration of CFU / ml vaccine is evenly sprayed on the surface of the particles using a high-pressure spray gun. Vaccine spray amount 10 7 CFU / g. (that is, each gram of sprayed particles contains 10 7 CFU)

[0072] The second step of coating the isolation layer: the dry powder composition A was dissolved in water to prepare a suspension with a solid content of 8%, and evenly sprayed on the surface of the vaccine layer with a high-pressure spray gun. The coating weight gain was 4%.

[0073] Dry powder composition A formula consists of:

[0074] Hypromellose E5 30g

[0075] Hypromell...

Embodiment 3

[0095] The functional dry powder film-forming composition of embodiment 3 fish enteric-coated vaccine

[0096] Preparation method: select 10-30 mesh starch granules and set aside.

[0097] The first step of vaccine spraying: the dry powder composition A is dissolved in water, configured into an aqueous suspension with a solid content of 5%, and then an appropriate amount of flounder ascites disease vaccine physiological saline solution (10 10 CFU / ml), prepared into 10 8 The suspension at the concentration of CFU / ml vaccine is evenly sprayed on the surface of the particles using a high-pressure spray gun. Vaccine spray amount 10 8 CFU / g.

[0098] The second step of coating the isolation layer: Dissolve the dry powder composition A in water to prepare a suspension with a solid content of 10%, and use a high-pressure spray gun to evenly spray it on the surface of the vaccine layer. The coating weight gain was 4%.

[0099] The formula of dry powder composition A consists of: ...

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Abstract

The invention relates to an enteric-coated oral vaccine for fishing, belonging to the field of oral vaccines for fishing. The enteric-coated oral vaccine for fishing comprises particles or pellets sprayed with a vaccine layer and sequentially coated with an isolating layer and an enteric-coated layer outside the vaccine layer. A dry powder composition A for spraying vaccine and coating the isolating layer comprises the following components (by weight parts) one or more film-forming agents selected from hydroxypropyl methylcellulose, polyvinyl alcohol and polyvinyl pyrrolidone 40-60 parts, one or more plasticizing agents selected from phosphoglyceride, triethyl citrate and polyethylene glycol 3-10 parts, one or more antisticking agents selected from silicon dioxide, magnesium stearate, talcum powder and titanium oxide 30-50 parts, and surfactant selected from lecithin and Tween-80 0-5 parts. The vaccine can be directionally released in intestinal tract.

Description

technical field [0001] The invention belongs to the field of fish oral vaccines. Background technique [0002] Diseases of farmed fish are increasing year by year, causing huge economic losses and seriously hindering the development of aquaculture. At present, the main means of controlling various diseases is extensive and large-scale input of chemical drugs and antibiotics. As a result of long-term and large-scale use of chemical drugs and antibiotics, environmental pollution problems, pathogenic and non-pathogenic microbial resistance problems, As well as food safety issues with excessive drug residues; for example, the turbot incident is a typical example, which has attracted close attention from many parties. At present, the state has banned the production and use of seriously harmful drugs. Improving the ability to prevent and control diseases in aquaculture is imminent. With the requirements of green food and environmental protection and the development of immunolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K9/28A61K47/38A61P37/00
Inventor 孙金生王守斌耿绪云韩超王雪惠董学旺李翔
Owner 天津市水产研究所
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