Injection type tissue engineering bone renovation material and construct method thereof

A tissue engineering bone and repair material technology, applied in medical science, prosthesis, etc., can solve problems such as lack of biomechanical strength, achieve good biodegradability, good biocompatibility, and no toxic side effects

Inactive Publication Date: 2010-03-24
NAN FANG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most of the materials used for injectable bone are gels, mainly including fibrin gels, polyethylene oxide hydrogels, alginate gels, etc. These biomaterials are gel-like after being injected into the body and do not have Biomechanical strength, although the composite seed cells have a certain osteogenic effect after implantation in the body, there is still a considerable distance from the ideal injectable bone scaffold material

Method used

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  • Injection type tissue engineering bone renovation material and construct method thereof
  • Injection type tissue engineering bone renovation material and construct method thereof
  • Injection type tissue engineering bone renovation material and construct method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment one: the cultivation of seed cell

[0031] Take 5-month-old healthy Chinese green goats (weight 35.0-40kg, male or female, provided by the Animal Experiment Center of Southern Medical University), perform iliac crest puncture with a 16-gauge bone puncture needle under aseptic operation after anesthesia, and extract bone marrow by multi-point puncture 10 mL, centrifuged and filtered with a 100-mesh stencil, inoculated into a culture bottle, added high-sugar DMEM medium (Hyclone, USA) containing 10% fetal bovine serum (Hyclone, USA), and placed in CO 2 Cultivate in an incubator (Harris Company, U.S.), change the medium every 3 days, and treat the primary cells to cover the bottom of the bottle, digest and separate the adherent cells with 0.25% trypsin-0.02% DMEM solution, add conditioned medium (DMEM+ 10% fetal bovine serum + dexamethasone 10-8mol / L + β-sodium glycerophosphate 10mmol / L + ascorbic acid 50mg / L), put in CO 2 The incubator continued to cultivate, ...

Embodiment 2

[0032] Example 2: Compounding of seed cells and injectable materials

[0033] The chitosan used in the experiment is a liquid material, and β-tricalcium phosphate (β-TCP) is a solid material (provided by the Department of Materials Science and Engineering of Jinan University). After 15 minutes, it gradually solidified into a solid state. In this experiment, 3ml of chitosan and 1.8g of β-TCP were compounded in vitro. After curing and forming, they were cut into 4mm×4mm×3mm sizes with a sterile scalpel for use. Take the third generation BMSCs (planting density 2×10 4 / hole) were inoculated in 24-well culture plates, and 1ml of medium was added to each well. The experiment was divided into two groups, namely the material group and the blank control group. In the experimental group, 4mm×4mm×3mm chitosan-β-TCP composite material was mixed with the cells in each hole, and the cells were simply inoculated in the control group, and treated at different times for MTT detection. After...

Embodiment 3

[0034] Embodiment 3: Inverted Microscope Observation

[0035] The growth of cells in each group was observed daily using an inverted microscope.

[0036] Results: 4 hours after cell inoculation, the cells were partially adhered to the wall, and 12 hours later, the cells were completely adhered to the wall, and the cells were polygonal and spindle-shaped. The cells grow faster, and cell clones form in 2 to 3 days, and merge into a single layer after 8 to 10 days. The growth of the cells in the material group is similar to that of the control group (such as figure 1 and figure 2 shown), the cell morphology was normal.

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Abstract

The invention relates to an injection organism repair material and relative production. Wherein, it comprises carrier support and seed cell adhered on the carrier support to form skeleton three-dimension structure and biological active composite; and it uses the injection chitose-beta-tricalcium phosphate composite as carrier support to couple seed cell; the seed cell is bone marrow substrate stemcell. The test has proved that the chitose-beta-tricalcium phosphate composite has better compatibility with BMSCs.

Description

technical field [0001] The invention belongs to the technical field of preparing artificial organs by tissue engineering methods in biomedical engineering, and in particular relates to an injection-type tissue engineering bone repair material and a construction method thereof. Background technique [0002] Injectable tissue-engineered bone is a liquid-state injectable tissue-engineered bone prepared by using degradable biomaterials that are initially liquid and organically compounded with seed cells, and gradually solidifies to form a gel during the process of completely filling the bone defect. Or solid-state tissue-engineered bone to repair bone defects. Injectable bone is easy to shape, easy to compound with seed cells and growth factors, and has the advantages of less tissue damage, no damage to the blood supply of the repair area, and easy operation. Many scholars at home and abroad pay close attention to it. At present, most of the materials used for injectable bone ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/00
Inventor 裴国献金丹程文俊
Owner NAN FANG HOSPITAL
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