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Two flavone glycosides in dandelion and medical use for against Gram-positive bacterium thereof

A technology of flavonoid glycosides and medicinal salts, applied in the field of pharmaceutical compositions containing these compounds, can solve the problems of unclear chemical composition and the like

Active Publication Date: 2009-07-08
DALI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few domestic research reports on the chemical constituents of Dandelion plants, and the chemical constituents of the medicinal dandelion Taraxacum officinale are mostly cited. The current search results found that Ling Yun’s research group has researched and reported on the chemical constituents of Mongolian Dandelion [Ling Yun, Bao Yanyan, Zhu Lili, Zheng Junhua, Cai Shaoqing, Xiao Yue, Research on Chemical Constituents of Dandelion, Chinese Journal of Pharmaceutical Sciences, 1997, 32, 584-586; Ling Yun, Bao Yanyan, Zhang Yonglin, Cai Shaoqing, Zheng Junhua, Research on Chemical Constituents of Dandelion, Journal of Naval General Hospital, 1998, 11, 167- 169], a total of 5 compounds were obtained: β-sitosterol, quercetin, caffeic acid, chlorogenic acid and luteolin-7-oxo-glucoside, while other chemical components are not yet clear

Method used

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  • Two flavone glycosides in dandelion and medical use for against Gram-positive bacterium thereof
  • Two flavone glycosides in dandelion and medical use for against Gram-positive bacterium thereof
  • Two flavone glycosides in dandelion and medical use for against Gram-positive bacterium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Separation of Mongolian Taraxacin A and Mongolian Taraxacin B:

[0024] 1.1 Instruments and reagents

[0025] The melting point was measured with a micro melting point apparatus (produced by Beijing Tektronix Co., Ltd.), and the temperature was not corrected; the optical rotation was measured on the Polax-2L type automatic optical rotation made in Japan; ; Ultraviolet spectrum is measured with Shimadzu UV-240 ultraviolet spectrophotometer; Proton nuclear magnetic resonance spectrum ( 1H-NMR), carbon nuclear magnetic resonance spectrum ( 13 C-NMR) and two-dimensional nuclear magnetic resonance (2D NMR) were determined by INOVA superconducting nuclear magnetic resonance spectrometer (VARIAN INOVA-400 MHz) (tetramethylsilyl ether TMS as internal standard); electrospray mass spectrometry (ESI-MS ) was measured by Bruker Esquire 3000+ mass spectrometer, silica gel GF254 (10-40 mesh) for column chromatography (100-200 mesh, 200-300 mesh and 300-400 mesh) for thi...

Embodiment 2

[0047] Example 2: Detection of the Ability of Flavonoid Glycosides 1 and 2 to Inhibit Gram-positive Bacteria

[0048] 2.1 Principle:

[0049] The "tube-and-disk method" is used to study the in vitro antibacterial activity of the test drug, which is to use the test drug added to the Oxford cup to diffuse into the culture medium of the inoculated test bacteria, thereby inhibiting the growth of the bacteria. The diameter of the bacterial circle was used to determine the antibacterial activity of the test drug.

[0050] 2.2 Detection of bacteria:

[0051] Staphylococcus aureus 26003-23 ​​was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0052] Beta-hemolytic Streptococcus 32210 was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0053] 2.3 Test drugs:

[0054] DMSO: DMSO solvent control;

[0055] Norfloxacin: 1.25mg / ml;

[0056] Mongolian dandelion A (compound 1): 5.0mg / ml; Mongolian ...

Embodiment 3

[0072] Example 3: Comparison of Compounds 1 and 2 in Rhizomes and Shoots and Leaves of Five Species of Dandelion

[0073] Dandelion (Mongolian dandelion) (Taraxacum mongolicum Hand-Mazz), Alkaline dandelion (Hua dandelion) (Taraxacum sinicum Kitag), Jehol dandelion (White margin dandelion) (Taraxacum platypecidum Diels), Northeast dandelion (Taraxacum ohwianumKitag), anti-bract dandelion (Taraxacum grypodon Dahlst) and Xing'an dandelion (Taraxacum falcilobum Kitag) each 20 grams were collected and identified by Professor Peng Hua from Kunming Institute of Botany, Chinese Academy of Sciences.

[0074] Waters high performance liquid chromatography (Waters HPLC, 2695 separation system includes quaternary pump, column thermostat autosampler; 2996 ultraviolet diode matrix detector, Empower chromatographic operating software and Waters RP-184.6mm×25mm, 5μm chromatographic column) to detect the content of public compounds 1 and 2.

[0075] Treatment of test samples: Weigh 0.5 g (...

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Abstract

The present invention relates to flavonoid glycosides isolated from dandelion and capable of preventing and treating Staphylococcus aureus and beta-hemolytic streptococcus infection diseases and their pharmaceutically acceptable salts, their anti-infective medicinal uses, and pharmaceutical compositions containing these compounds. The two novel flavonoid glycoside compounds of the present invention have obvious effects of inhibiting the growth of Staphylococcus aureus and beta-hemolytic streptococcus, and can be expected to be used for the prevention and treatment of boils, pustules and toxic epidermal decomposition caused by the infection of these two. , pneumonia, osteomyelitis, acute myocarditis, endocarditis, meningitis, mastitis, cystitis, pelvic inflammatory disease, urinary tract inflammation, prostatitis, bacteremia, toxic shock syndrome, lymphadenitis, arthritis, pharyngitis , cervicitis, and muscle, skin, urogenital tract, central nervous system abscesses.

Description

technical field [0001] The present invention relates to the field of medical technology. Specifically, the present invention relates to two novel flavonoid glycosides isolated from dandelion that can prevent and treat Staphylococcus aureus and / or beta-hemolytic streptococcus infection and their medicinal properties. Salts, their use in anti-infective medicines, and pharmaceutical compositions containing these compounds. It is expected to be used in the prevention and treatment of boils, pustules, toxic epidermis, pneumonia, osteoarthritis, acute myocarditis, endocarditis, meningitis, mammary inflammation, cystitis, pelvic inflammatory disease, urinary tract inflammation, prostatitis, bacteremia, toxic shock syndrome, lymphadenitis, arthritis, pharyngitis, cervicitis, and muscle, skin, urogenital tract, central nervous system abscess. technical background [0002] Staphylococcus aureus, a Gram-positive coccus, and methylcillin-resistant Staphylococcus aureus (MRSA), a strai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61K36/288A61P31/04
Inventor 李峰施树云徐艳陶巧凤关静宋月晗曾苏赵昱
Owner DALI PHARMA
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