The invention discloses a nephrological nursing puncture sampling device. The nephrological nursing puncture sampling device comprises a base, the lower end of the base is provided with wheels, the base is provided with a fixing box, the upper end of the fixing box is provided with a lifting box, the upper end of the lifting box is provided with a lifting base, and the upper end of the lifting base is provided with a bed board; a first sliding seat is arranged inside the lifting seat, the upper end of a first threaded slider is connected with the bed board, one side of the base is provided with a stand rack, the upper end of the stand rack is provided with a second sliding seat, the lower end of a second threaded slider is connected with a third sliding seat, the lower end of a third threaded slider is connected with an electric pushrod, the lower end of the electric pushrod is connected with a turntable rack, inside which a turntable device is arranged, and the turntable device is connected with a rotating rack which is provided with a puncture fixator. The nephrological nursing puncture sampling device is convenient to move, capable of achieving adjustment of different dimensions, convenient to fix and adjust, capable of achieving the functions of lifting adjustment and horizontal movement and facilitating puncture position selection.