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Rhamnolipid crude extract prepared by fermenting food and rink waste oil and application thereof

A technology for rhamnolipid and catering waste oil, which is applied in the fields of fermentation, biological water/sewage treatment, water/sludge/sewage treatment, etc. The problem of high production cost, to achieve the effect of shortening the processing time, shortening the processing time, and reducing the production cost

Inactive Publication Date: 2007-02-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The fermentation of rhamnolipids generally uses various vegetable oils or n-hexane as carbon sources, and the fermentation cycle generally takes about 7-11 days, which makes the production cost relatively high. Among them, the production process and carbon source cost account for the entire production cost. More than 30%, restricting the industrialized large-scale production of rhamnolipids, and when using waste materials such as saponin slices to prepare glycolipids, although the cost of raw materials has been reduced, because the output is very low, industrialized production cannot be realized

Method used

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  • Rhamnolipid crude extract prepared by fermenting food and rink waste oil and application thereof
  • Rhamnolipid crude extract prepared by fermenting food and rink waste oil and application thereof
  • Rhamnolipid crude extract prepared by fermenting food and rink waste oil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the shake flask culture of natural bacterial strain and mutagenized bacterial strain:

[0033] Divide the 50ml shake flasks into two batches of ten groups, add activated 3% ultraviolet mutagenized Pseudomonas aeruginosa ZJU. Bacteria; the non-mutated strain used was naturally screened from the soil of the refinery sewage outlet, and was confirmed to be Pseudomonas aeruginosa after testing its 16sRNA, and the ultraviolet mutagenic strain was based on this strain as the starting bacteria after 15W ultraviolet light The high-yield strain screened after 30 seconds of lamp irradiation was named Pseudomonas aeruginosaZJU.u1.

[0034] a. Medium composition (g / L): NaNO 3 , 4.0; NaCl, 1.0; KCl, 1.0; CaCl 2 2H 2 O, 0.1; KH 2 PO 4 , 3.0; Na 2 HPO 4 12H 2 O, 3.0; MgSO4, 0.2; FeSO 4 ·7H 2 O, 0.001; trace elements, 2mL / L, composition (g / L): FeCl 3 ·6H 2 O 0.08, ZnSO 4 ·7H 2 O 0.75, CuSO 4 ·5H 2 O 0.075, MnSO 4 ·H 2 O 0.75, H 3 BO 3 , 0.15;

[0035] ...

Embodiment 2

[0040] Embodiment 2: Preparation of rhamnolipid powdery crude product (1)

[0041] 1. Strain: the fermentation strain is Pseudomonas aeruginosa ZJU.U1 (Pseudomonas aeruginosa ZJU.u1)

[0042] 2. Place the strain in glycerin medium and activate it in a shaker for 48-72 hours to obtain a sufficient inoculum size for fermentation. The activation temperature is 36-37°C. The pH is controlled between 6.5-7.0, and the shaking time is 300rpm.

[0043] 3. The composition of the activation medium is as follows (g / L):

[0044] Glycerol, 30; NaNO 3 , 4.0; NaCl, 1.0; KCl, 1.0; CaCl 2 2H 2 O, 0.1; KH 2 PO 4 , 3.0; Na 2 HPO 4 12H 2 O, 3.0; MgSO 4 , 0.2; FeSO 4 ·7H 2 O, 0.001; trace elements, 2mL / L, composition (g / L): FeCl 3 ·6H 2 O 0.08, ZnSO 4 ·7H 2 O 0.75, CuSO 4 ·5H 2 O 0.075, MnSO 4 ·H 2 O 0.75, H 3 BO 3 , 0.15

[0045] 4. Production of rhamnolipids in fermentation tanks

[0046] a. Medium composition (g / l): NaNO 3 , 5.0; NaCl, 1.0; KCl, 1.0; CaCl 2 2H 2 O, 0.0...

Embodiment 3

[0050] Embodiment 3: the preparation of rhamnolipid fermentation broth (two)

[0051] 1, bacterial classification: with embodiment 1

[0052] 2, activation: with embodiment 1

[0053] 3. Production of rhamnolipids in fermentation tanks

[0054] a. Medium composition (g / l): NaNO 3 , 2.0; NaCl, 5.0; KCl, 0.5; CaCl 2 2H 2 O, 0.03; KH 2 PO 4 , 2.0; Na 2 HPO 4 12H 2 O, 6.0; MgSO 4 , 0.05; FeSO 4 ·7H 2 O, 0.005; trace elements, 2mL / L, composition (g / L): FeCl 3 ·6H 2 O 0.08, ZnSO 4 ·7H 2 O 0.75, CuSO 4 ·5H 2 O 0.075, MnSO 4 ·H 2 O 0.75, H 3 BO 3 , 0.15.

[0055] b. Waste oil content 30g / L

[0056] c. Fermentation conditions: 72 hours later, the amount of waste catering oil added was 0.125Kg, and the rest of the conditions were the same as in Example 1; the filtrate was directly used as the crude product of the rhamnolipid solution.

[0057] d. Sterilization of fermentation broth and preparation of crude extract: remove bacteria by microfiltration of 0.22 micro...

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Abstract

the invention discloses a manufacturing method of rough rhamnolipid extract and application fermented by waste food and drink oil, which is characterized by the following: proceeding ultraviolet mutagenesis for natural sieved pseudomonas; picking fitful bacteria; adopting waste food and drink oil as ferment carbon source; making the density of rhamnolipid reach 35-45g / L; ultrafiltrating the ferment liquid to remove bacteria to obtain the powder-shaped rough extract through spraying and drying method; adding 0.01-3% rough extract in the biochemical dirty water to improve disposing efficiency.

Description

technical field [0001] The invention relates to a preparation method and application of a biosurfactant, in particular to a rhamnolipid crude extract produced by fermenting waste catering oil and its application. Background technique [0002] As an anionic surfactant, the amphiphilicity of rhamnolipid molecules makes it oriented to form a monomolecular film on the surface of the solution (or oil-water interface). This directional adsorption characteristic makes it have many unique surface properties. Active, widely used in oil extraction, environmental protection, agriculture, cosmetics, food, medicine and industrial purification. [0003] The fermentation of rhamnolipids generally uses various vegetable oils or n-hexane as carbon sources, and the fermentation cycle generally takes about 7-11 days, which makes the production cost relatively high. Among them, the production process and carbon source cost account for the entire production cost. More than 30%, restricting the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/00C09K3/32C02F3/00A62D3/00
Inventor 孟琴章宏梓朱湧
Owner ZHEJIANG UNIV
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