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Calcium ion content determination method and calcium ion diagnosis kit

A technology of a diagnostic kit and a determination method, applied in the field of medical inspection and determination, can solve problems such as high cost, and achieve the effects of good accuracy and accurate test results

Inactive Publication Date: 2006-04-26
王尔中
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

but the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Example 1 (single dose)

[0097] The ionized calcium diagnostic kit of this embodiment includes:

[0098] Buffer 80mmol / l

[0099] Phospholipids 2mmol / l

[0100]Phospholipase (requires calcium ion activation) 20000U / l

[0101] Choline Oxidase 2000U / l

[0102] Peroxidase 2000U / l

[0103] Stabilizer 50% (total volume)

[0104] 4-Amino arsenic resistance 2mmol / l

[0105] Carbolic acid 10mmol / l

[0106] Set on the automatic biochemical analyzer: the temperature is 37°C, the reaction time is 10 minutes, the main test wavelength is 495-505nm, the test sub-wavelength is above 600nm, the volume ratio of the tested ion calcium sample to the reagent is 1 / 25, and the reaction direction is Positive reaction.

[0107] After the sample and reagents are added and allowed to mix, the following reaction occurs:

[0108] Phospholipids + water Phospholipase-D / Ca ++ Phosphatidic acid + choline

[0109] Choline + 2 Oxygen + Water bile phosphate oxidase Betaine + 2 Hydroge...

Embodiment 2

[0114] Embodiment 2 (double dose)

[0115] The ionic calcium diagnostic reagents of the present embodiment include:

[0116] Reagent I

[0117] Buffer 100mmol / l

[0118] Choline Oxidase 11000U / l

[0119] Peroxidase 11000U / l

[0120] Stabilizer 50% (total volume)

[0121] 4-Amino arsenic resistance 1mmol / l

[0122] Reagent II

[0123] Buffer 100mmol / l

[0124] Phospholipids 6mmol / l

[0125] Phospholipase (requires calcium ion activation) 110000U / l

[0126] Stabilizer 50% (total volume)

[0127] 2,4,6-Tribromo-3-hydroxy-benzenesulfonic acid 1mmol / l

[0128] When measuring the content of ionized calcium, the temperature is controlled at 30°C, the reaction time is 15 minutes, the test main wavelength is 546nm, the test sub-wavelength is above 630nm, the volume ratio of the tested ionized calcium sample to the reagent is 1 / 25, and the reaction direction is positive reaction.

[0129] The specific measurement steps are:

[0130] Phospholipids + water Phospholipase-D / Ca...

Embodiment 3

[0136] Embodiment three (three doses)

[0137] The ionic calcium diagnostic reagents of the present embodiment are three doses, including:

[0138] Reagent I

[0139] Buffer 120mmol / l

[0140] Stabilizer 20mmol / l

[0141] 3-Methyl-2-benzothiazolone hydrazone 2mmol / l

[0142] Reagent II

[0143] Buffer 120mmol / l

[0144] Choline oxidase 20000U / l

[0145] Peroxidase 20000U / l

[0146] Stabilizer 50% (total volume)

[0147] Reagent III

[0148] Buffer 120mmol / l

[0149] Phospholipids 10mmol / l

[0150] Phospholipase (requires calcium ion activation) 200000U / l

[0151] Stabilizer 50% (total volume)

[0152] Dimethylaniline 2mmol / l

[0153] Set on the automatic biochemical analyzer: the temperature is 25°C, the reaction time is 20 minutes, the test main wavelength is 578nm, the test sub-wavelength is above 700nm, the volume ratio of the tested ion calcium sample to the reagent is 1 / 25, and the reaction direction is positive reaction .

[0154] The specific measurement ...

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Abstract

The present invention relates to ionic calcium content determining method and ionic calcium diagnosis kit, and belongs to the field of medical detecting technology. The kit includes buffering solution, phospholipid, phosphatidase, choline oxidase, hydrogen peroxidate, aldehyde stabilizer and reducing chromogen. Through mixing the sample and reagent in certain volume ratio to generate enzyme coupling reaction, detecting the main wavelength absorbance variation of the reaction product under biochemical analyzer and calculation, ionic calcium content is obtained. The present invention has the advantages of high sensitivity, high precision and no contamination of inner and outer matters.

Description

technical field [0001] The invention relates to a method for measuring the content of ionized calcium, and at the same time, the invention also relates to an ionized calcium diagnostic kit for realizing the method, which belongs to the technical field of medical examination and determination. Background technique [0002] There are many methods for the determination of total calcium, which can be summarized as titration (including redox titration, complexometric titration), colorimetric method (the most commonly used and convenient for automated operation are o-cresolphthalein complex ketone method, methyl thymol). Blue method, arsine azo III method, etc.), flame photometry, atomic absorption spectrophotometry, isotope dilution mass spectrometry. The decisive method for calcium determination recommended by the International Federation of Clinical Chemistry (IFCC) is isotope dilution mass spectrometry (IDMS), and the reference method is atomic absorption spectrophotometry. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/44
Inventor 王尔中
Owner 王尔中
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