Anemia screening kit for CRISPR and CAS9 targeted capture of long fragment DNA and method thereof
A targeted capture and long-fragment technology, applied in recombinant DNA technology, DNA/RNA fragments, biochemical equipment and methods, etc., can solve problems such as limited throughput of sequencing detection, complex primer design, and manual analysis of detection results. Achieve the effects of avoiding non-specific amplification and PCR preference, improving sequencing accuracy and good detection advantages
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[0077] Example 1: Detection and verification of thalassemia gene mutation;
[0078] A total of 1257 blood samples diagnosed with thalassemia were collected from a certain area, including 302 healthy people, 589 α-thalassemia cases, 337 β-thalassemia cases, and 22 cases carrying both α and β thalassemia mutations. There were 4 cases of delta thalassemia and 3 cases of delta beta thalassemia.
[0079] Use this method to test these screen-positive samples;
[0080] At the same time, the α-thalassemia gene detection kit (Gap-PCR method) was used to detect 3 common deletion genes of α-thalassemia (-SEA, -α3.7 and -α4.2);
[0081] Non-deletion α-thalassemia gene mutation detection kit (PCR-reverse dot blot method) to detect 3 gene mutations of α-thalassemia (αCSα, αWSα and αQSα);
[0082] β-thalassemia gene detection kit (PCR-reverse dot blot method) detects 17 common gene mutations in β-thalassemia (CD41-42, CD17, IVS-II-654, CD26CD71-72, IVS-I-1, - 28, CD31, CD43, -29, CD27-28,...
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