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3D printing fibroin annular stent reinforced collagen-based corneal repair material and preparation method thereof

A 3D printing and stent reinforcement technology, applied in additive processing, prosthesis, coating, etc., can solve problems such as tearing, lack of natural corneal structure and performance, and achieve improved mechanical properties, good physical and chemical properties, and wide application foreground effect

Inactive Publication Date: 2022-07-12
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current common collagen-based artificial corneas often do not have the complex structure and performance of natural corneas, so during the transplantation process, when the suture shear force exceeds a certain level, it will tear

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Using collagen, silk fibroin and citric acid as raw materials, a 3D printed silk fibroin annular scaffold reinforced collagen-based corneal repair material was prepared. The preparation steps of this silk fibroin-enhanced collagen-based corneal repair material are as follows:

[0022] (1) First, prepare a silk fibroin solution with a concentration of 30% by mass, design the parameters of a 3D silk fibroin ring scaffold with a regular octagonal structure, load the silk fibroin solution into a 3 mL syringe, and dispense the nozzle through No. 33 chamfering Extruded into a glass dish equipped with a water-salt bath (4M sodium chloride and 0.5M potassium phosphate), and the printing speed and pressure were controlled to be 1.2mm.s-1 and 210kPa, respectively, to construct a three-dimensional structure with a regular octagon. Silk fibroin ring scaffold material;

[0023] (2) Then, prepare a 5.0% mass concentration citric acid solution, and chemically modify the surface of th...

Embodiment 2

[0027] Using collagen, silk fibroin and citric acid as raw materials, a 3D printed silk fibroin annular scaffold reinforced collagen-based corneal repair material was prepared. The preparation steps of this silk fibroin-enhanced collagen-based corneal repair material are as follows:

[0028] (1) First, prepare a silk fibroin solution with a concentration of 35% by mass, design the parameters of a 3D silk fibroin ring scaffold with a regular dodecagonal structure, load the silk fibroin solution into a 5 mL syringe, and distribute it through a 33-gauge chamfer The nozzle was extruded into a glass dish containing a water-salt bath (4M sodium chloride and 0.5M potassium phosphate), and the printing speed and pressure were controlled to be 1 mm.s-1 and 210 kPa, respectively, to construct a three-dimensional filament with a regular dodecagon. Veg protein ring scaffold material;

[0029] (2) Then, a 5.5% mass concentration citric acid solution was prepared, and the 3D printed silk f...

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Abstract

The preparation method comprises the following steps: 1) preparing a silk fibroin solution, designing and optimizing 3D printing parameters of a silk fibroin annular stent, and constructing a plurality of silk fibroin annular stent materials with regular three-dimensional space structures; 2) carrying out surface chemical modification on the obtained silk fibroin annular scaffold material through a citric acid molecular solution; (3) preparing a collagen solution, introducing the carboxylated fibroin annular scaffold obtained in the step (2) into a collagen solution system, and enabling fibroin molecules and collagen molecules to form amido bond combination by taking EDC and NHS as a cross-linking agent and a catalyst respectively, so as to obtain a collagen solution; and finally, the silk fibroin reinforced collagen-based cornea repair material which is doubly compounded on a macroscopic physical structure and a microscopic chemical assembly level is formed. The material obtained by the invention has better physical and chemical properties and biocompatibility, is controllable in molding, and can be used for clinical repair and treatment of corneal tissue defects.

Description

technical field [0001] The invention relates to a 3D printing silk fibroin ring-shaped scaffold reinforced collagen-based corneal repair material and a preparation method thereof, in particular to a method for preparing a collagen-based corneal repair material with good physical and chemical properties and biocompatibility. The material obtained by the invention can be applied to the repair and treatment of corneal tissue defects in clinic. Background technique [0002] Corneal disease is a very common eye disease, and about one-third of blind patients are caused by corneal disease. For patients with corneal disease, corneal transplantation is required to replace the damaged cornea with a healthy cornea from a donor, which has led to a great demand for allogeneic corneas. However, in clinical practice, the number of healthy corneal donors is far less than the demand for corneal transplantation, so the research and development of artificial corneas is very necessary. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/34A61L27/50B33Y10/00B33Y70/00B33Y80/00C08H1/00
CPCC08H1/00A61L27/227A61L27/34A61L27/50B33Y70/00B33Y80/00B33Y10/00A61L2430/16
Inventor 刘杨
Owner CHANGZHOU UNIV
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