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Liquid fermentation culture medium of brevibacterium friariorum SDB5

A cold-resistant Brevibacterium, liquid fermentation technology, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of lack of organic matter, medium/trace elements, high production cost, low fermentation density, etc., to improve nutrient utilization efficiency, The effect of shortening the fermentation cycle and promoting plant growth

Pending Publication Date: 2022-06-07
榆林市中泰农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Today's cultivated land soil in China has the following six problems: (1) the quality of cultivated land is declining, and it is polluted by heavy metals, pesticides, and inferior chemical fertilizers; (2) the soil is severely drought, desertification, salinization, and acidification; / trace elements; (4) more than 50% of nitrogen loss, phosphorus and potassium are fixed and unused; (5) crops are planted continuously, leading to aggravation of soil-borne diseases; (6) soil fertility decreases, microbial activity is poor, and productivity decreases, The above problems seriously restrict the development of China's green agriculture and ecological agriculture
At present, expensive materials such as yeast powder, tryptone, and analytically pure sodium chloride are mainly used to cultivate Brevibacterium cold-resistant SDB5. The production cost is high and the fermentation density is low, which is not conducive to the production and application of related products. Therefore, looking for Materials with low price and sufficient nutrient content are used for fermentation production, and the number of functional bacteria in the fermented product is continuously increased, which is an urgent problem to be solved

Method used

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  • Liquid fermentation culture medium of brevibacterium friariorum SDB5

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Medium optimization of Brevibacterium hardy SDB5

[0019] The yeast extract was replaced with yeast paste, and NaCl was replaced with pickled coarse salt (the main component was sodium chloride). On this basis, a single-factor replacement experiment was performed. In this experiment, LB medium was used as the control group, and its components were: 10g / L tryptone, 5g / L yeast extract, 10g / L NaCl.

[0020] 1.1 Single factor influence

[0021] (1) Experiment of pickling coarse salt instead of analytically pure sodium chloride

[0022] Experimental group: peptone medium: 10g / L peptone, 5g / L yeast extract, 10g / L coarse salt. The bacterial culture conditions were the same. Using a spectrophotometer to measure the OD of the bacterial liquid at different incubation times 600 The value of bacteria was measured, and the bacterial content in the bacterial liquid was detected to judge the growth of bacteria. The experimental results are shown in Table 1.

[0023] Ta...

Embodiment 2

[0045] Example 2: Sodium chloride removal experiment

[0046] Coarse salts were removed from the medium most suitable for SDB5 bacteria, and inorganic salts were directly added for comparative experiments, as shown in Table 7. The control group is LB medium (numbered 4), and the results are shown in the results table of the plate counting experiment, as shown in Table 8. It can be seen from Table 8 that NaCl has a significant impact on SDB5 bacteria and cannot be directly replaced by inorganic salts, otherwise the growth ability of the strain will be weakened, thereby reducing the bacterial content in the fermentation broth.

[0047] Table 7 Plate Counting Experiment Design Table (unit: g / L)

[0048]

[0049] Table 8 Plate Counting Experiment Results Table

[0050]

Embodiment 3

[0051] Example 3: Optimal medium composition and plate count experiment

[0052] Based on the above experimental results, it can be seen that the most suitable special low-cost high-density liquid fermentation medium for Brevibacterium cold-resistant SDB5 is: 8g / L soybean meal, 2g / L krill meal, 5g / L yeast extract, 10g / L pickled Coarse salt, 1.8g / L MgSO 4 , 1.8g / L K 2 HPO 4 and 1.2g / L CaCO 3 . Fermentation experiments were carried out using this medium, and the control group was LB medium, and the fermentation conditions were the same. The results showed that the viable bacteria content of Brevibacterium cold resistant SDB5 in the fermentation broth of the control group was 0.8×10 8 CFU / mL, while the viable bacterial content of Brevibacterium cold-resistant SDB5 in the fermentation broth of the optimal medium was 3.42×10 8 CFU / mL, the fermentation effect of the optimal medium was about 4.3 times higher than that of the control group, and the effect was good.

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Abstract

The invention relates to a liquid fermentation culture medium of brevibacterium friaricola SDB5. The special low-cost and high-density liquid fermentation culture medium for the brevibacterium friaricola SDB5 comprises 8 g / L of soybean meal, 2 g / L of krill meal, 5 g / L of yeast extract, 10 g / L of pickling crude salt, 1.8 g / L of MgSO4, 1.8 g / L of K2HPO4 and 1.2 g / L of CaCO3. And the viable organism content of the brevibacterium brevicola SDB5 in the fermentation liquor reaches 3.42 * 10 < 8 > CFU / mL. The liquid fermentation culture medium of brevibacterium friaricola SDB5 effectively overcomes the defects of low biomass, long fermentation period, high production cost and the like in the traditional fermentation process, is safe and non-toxic, can effectively improve the nutrient utilization efficiency, remarkably promote plant growth, assist fertilizer reduction, increase the crop yield and improve the crop quality, is beneficial to large-scale popularization and application, and has wide application prospects. The brevibacterium friaricola SDB5 microbial inoculum can be widely applied to agricultural production as a bio-fertilizer.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to a liquid fermentation medium of Brevibacterium cold-resistant SDB5. Background technique [0002] There are six problems in China’s arable land soil today: (1) The quality of arable land is declining and polluted by heavy metals, pesticides, and inferior chemical fertilizers; (2) Soil aridity, desertification, salinization, and acidification are serious; (3) There is a general lack of organic matter, medium and / trace elements; (4) more than 50% nitrogen loss, phosphorus and potassium are fixed and unused; (5) continuous stubble planting of crops leads to aggravation of soil-borne diseases; (6) soil fertility declines, microbial activity is poor, and productivity declines, The above problems seriously restrict the development of green agriculture and ecological agriculture in China. [0003] Microbial fertilizers are inputs that meet the green requirements of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/13
CPCC12N1/20Y02P60/87Y02E50/10
Inventor 王建武刘畅相微微张宁宁张楠杨斌冯光惠
Owner 榆林市中泰农业科技有限公司
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