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SERS (Surface Enhanced Raman Scattering) virus detection chip for detecting novel coronavirus and preparation method of SERS virus detection chip

A coronavirus and chip technology, applied in the field of laser Raman spectroscopy and virus detection, can solve the problems of false negative test results, long detection time, and insufficient sensitivity of detection reagents, and achieve enhanced specificity, light weight, and good electrical insulation. Effect

Pending Publication Date: 2022-05-27
GUANGXI UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. The detection time is long, and the average single detection time takes 12 hours;
[0004] 2. The sensitivity of the detection reagent is not high enough, which can easily lead to false negative results

Method used

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  • SERS (Surface Enhanced Raman Scattering) virus detection chip for detecting novel coronavirus and preparation method of SERS virus detection chip
  • SERS (Surface Enhanced Raman Scattering) virus detection chip for detecting novel coronavirus and preparation method of SERS virus detection chip
  • SERS (Surface Enhanced Raman Scattering) virus detection chip for detecting novel coronavirus and preparation method of SERS virus detection chip

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Effect test

Embodiment 1

[0066] A SERS virus detection chip for detecting the new coronavirus, such as figure 1 As shown, it includes a SERS substrate, a graphene 3 arranged on the SERS substrate, and an ACE2 bioprobe 4 arranged on the graphene 3, and the SERS substrate includes Si / SiO as a base 2 Wafer 1 and metal nanostructures 2 above it.

[0067] The metal nanostructure 2 is a gold nanodisc array structure, which is made of Si. 3 N 4 The method of direct metal evaporation on the mask plate prepares periodic gold nanodiscs and nanopore materials, and the gold nanodisc array is formed by a plurality of nanocolumns perpendicular to the substrate periodically arranged, and the pore size is the same, The thickness is 45 nm or 50 nm or 55 nm and the root diameter is 45 nm or 50 nm or 55 nm.

[0068] The graphene 3 is a single-layer CVD graphene with a thickness of 0.3 nm or 0.325 nm or 0.35 nm, a length of 500 μm and a width of 500 μm.

[0069] The ACE2 bioprobe 4 is efficiently immobilized on the g...

Embodiment 2

[0071] A preparation method of a SERS virus detection chip, which is the preparation method of a SERS virus detection chip for detecting a novel coronavirus according to Embodiment 1, and the specific steps are:

[0072] (1) In Si / SiO 2 Metal nanostructures are prepared on the wafer to form a SERS substrate;

[0073] That is, the metal nanostructures are deposited on the wafer by the simple, convenient and economical mask direct metal evaporation preparation method in the physical preparation method (see image 3 ), thereby immobilizing metal nanostructures on Si / SiO cleaned for many times 2 On the wafer substrate, the specific steps are:

[0074] ①If figure 2 shown, select Si with the same diameter size and periodically arranged pore-like structure 3 N 4 Film 5 is used as a mask, which is fixed on the cleaned Si / SiO with Kapton tape with no glue residue. 2 on wafer 1;

[0075] ②If image 3 shown, the Si / SiO 2 The wafer was placed in a magnetron sputtering apparatus,...

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Abstract

The SERS virus detection chip for detecting the novel coronavirus comprises an SERS substrate, graphene arranged on the SERS substrate and an ACE2 biological probe arranged on the graphene, and the SERS substrate comprises a Si / SiO2 wafer serving as a base and a metal nanostructure above the Si / SiO2 wafer; the preparation method comprises the following specific steps: (1) preparing a metal nanostructure on a Si / SiO2 wafer to form an SERS substrate; (2) transferring the single-layer CVD graphene to an SERS (Surface Enhanced Raman Scattering) substrate; and (3) modifying ACE2 on graphene to form a new crown detection probe, thereby completing the preparation of the SERS virus detection chip. The ACE2 biological probe molecules and the graphene / periodic nano-metal structure SERS detection substrate are combined to form the new coronavirus biological detection chip, the detection chip is utilized to achieve high-sensitivity detection of the new coronavirus, the detection is rapid and efficient, and the capturing efficiency is higher.

Description

technical field [0001] The invention relates to the technical field of laser Raman spectroscopy and virus detection, in particular to a SERS virus detection chip for detecting novel coronavirus and a preparation method thereof. Background technique [0002] The 2019 new coronavirus (2019-nCOV) can be transmitted through respiratory droplets, contact, vertical mother-to-child transmission, fecal-oral transmission, and aerosol transmission. It has strong infectivity, strong mutation ability, and long incubation time. The symptoms are fever, cough, difficulty breathing, and severe cases may directly lead to kidney failure or even death. At present, the commonly used methods for screening and detecting the new coronavirus are mainly reverse transcription-polymerase chain reaction (RT-PCR) nucleic acid detection method and serum antibody detection including colloidal gold method and magnetic particle chemiluminescence method. Antibody detection can be used for nucleic acid detect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N33/569C12Q1/70C12R1/93B82Y30/00
CPCG01N21/658G01N33/56983C12Q1/70B82Y30/00G01N2333/165Y02A50/30
Inventor 王国富刘宴升邓峻鹏周瑾贾小波王小红韦秦明陈华金樊香所秦觅觅李红立常海欣高喜刘天行秦振乐
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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