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Preparation and application of mercury ion sensor based on photonic crystal enhanced fluorescence

A photonic crystal, enhanced fluorescence technology, used in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of expensive instruments, need professional operators, cumbersome detection process, etc., to reduce the detection concentration and detection limit, and enhance the detection signal. , the effect of high detection selectivity

Active Publication Date: 2016-02-24
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods generally have shortcomings such as cumbersome detection process, expensive instruments, high operating costs, complicated sample pretreatment, and the need for professional operators, and are not suitable for rapid on-site monitoring and continuous online analysis.

Method used

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  • Preparation and application of mercury ion sensor based on photonic crystal enhanced fluorescence
  • Preparation and application of mercury ion sensor based on photonic crystal enhanced fluorescence
  • Preparation and application of mercury ion sensor based on photonic crystal enhanced fluorescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1) Preparation of SiO by the improved StOber method 2 Spherical particles, prepared SiO with different particle sizes by adjusting the ratio of ammonia water and absolute ethanol 2 . Mix a certain amount of tetraethyl orthosilicate (TEOS) with absolute ethanol, and obtain a uniform TEOS solution by ultrasonication, add the above-mentioned TEOS solution to a certain amount of ammonia water and different proportions of absolute ethanol and water mixed solution, Magnetic stirring for 2 hours, centrifugal washing (washing with water and ethanol twice each), drying in a vacuum oven at 60°C for 24 hours to obtain SiO 2 Microspheres. The experimental parameters are as follows:

[0047]

[0048] 2) Synthesis of Opal photonic crystals by vertical deposition self-assembly method

[0049] The glass slides were ultrasonically cleaned in acetone, ethanol, and deionized water for 20 min, and then placed in a mixed solution (H 2 SO 4 and H 2 o 2 The volume ratio is 3:1) Soa...

Embodiment 2

[0059] 1) Synthesis of polystyrene microspheres

[0060] Pour a certain amount of purified styrene into a separatory funnel, wash with NaOH solution and ultrapure water in sequence, then remove a small amount of residual water with anhydrous calcium chloride, and finally obtain purified styrene by vacuum distillation. At room temperature, add styrene monomer, methyl methacrylate solution, acrylic acid, ultrapure water and sodium dodecylbenzenesulfonate into a three-necked flask together, stir at 630 / min, heat in a water bath to 50°C, and keep warm for 15 minutes Add ammonium persulfate dropwise and adjust the water bath to heat to 75°C, use an ice bath to cool to room temperature, and finally use an ice bath to cool to room temperature to complete the reaction to obtain a milky white polystyrene microsphere emulsion, wherein the styrene, methyl The volume ratio of methyl acrylate solution, acrylic acid, ultrapure water and sodium dodecylbenzenesulfonate is 20:1:1:100:123;

[...

Embodiment 3

[0073] 1) Synthesis of polystyrene microspheres

[0074] Pour a certain amount of purified styrene into a separatory funnel, wash with NaOH solution and ultrapure water in sequence, then remove a small amount of residual water with anhydrous calcium chloride, and finally obtain purified styrene by vacuum distillation. At room temperature, add styrene monomer, methyl methacrylate solution, acrylic acid, ultrapure water and sodium dodecylbenzenesulfonate into a three-necked flask together, stir at a speed of 660 / min, heat to 50°C in a water bath, and keep warm for 15 minutes Add the ammonium persulfate dropwise and adjust the water bath to heat to 85°C, use an ice bath to cool to room temperature, and finally use an ice bath to cool to room temperature to complete the reaction to obtain a milky white polystyrene microsphere emulsion, wherein the styrene, methyl The volume ratio of methyl acrylate solution, acrylic acid, ultrapure water and sodium dodecylbenzenesulfonate is 20:1:...

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Abstract

The invention provides preparation and application of a mercury ion sensor based on photonic crystal enhanced fluorescence. The mercury ion sensor is composed of photonic crystal, a nucleic acid fluorescence molecular probe and a DNA assistant probe. The photonic crystal is used as a substrate, and strong affinity between thymidine (T) of DNA and Hg<2+> is employed to allow fluorescence group ROX labeled aptamer and a complementary sequence to compose a fluorescence detection system. A photonic crystal film is formed on a glass sheet substrate by prepared PS microspheres through vertical deposition self-assembly. Gold is sprayed on the surface of the photonic crystal; and based on Au-S bonding effect, mercapto group modified fluorescence group ROX labeled aptamer undergoes self-assembly on the surface of the photonic crystal, wherein the emission wavelength of ROX accords with the forbidden band of the photonic crystal, so fluorescence enhancing effect is achieved. According to the invention, fluorescence signal intensity of a nucleic acid fluorescence molecule is improved by using the principles of enhancement of fluorescence through the photonic crystal, so sensitivity and accuracy of sensor detection are improved.

Description

technical field [0001] The invention belongs to the technical field of material preparation and analysis, and relates to a method for preparing photonic crystals with an opal structure by volatilization and self-assembly of PS pellets, in particular to the preparation and application of a mercury ion sensor based on photonic crystal-enhanced fluorescence. Background technique [0002] The photonic bandgap is the most important feature of photonic crystals. Photonic crystals have a structure similar to that of semiconductors, except that the periodically changing atoms in semiconductors are transformed into two dielectric materials with different dielectric constants that change periodically. Like semiconductor materials, the periodic arrangement of dielectric constants produces a certain "potential field". When the difference between the dielectric constants of the two materials is large enough, Bragg scattering will appear on the dielectric interface, resulting in a photon ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 温永强杨志林焦翔宇王文谦庞铎雷霞张学记
Owner UNIV OF SCI & TECH BEIJING
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