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Verticillium lecanii dispersible oil suspending agent as well as preparation method and application thereof

A technology of verticillium lecanii and dispersing oil, which is applied in the direction of botanical equipment and methods, applications, insecticides, etc., to achieve high suspension rate, improve storage stability, and perform well in wet sieving tests

Active Publication Date: 2022-04-12
NINGXIA UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the market is mainly based on the preparations of Beauveria bassiana and Metarhizium anisopliae, but there are few commercial preparations of Verticillium lecanii. It can be seen that there is an urgent need for research on the application of Verticillium lecanii

Method used

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  • Verticillium lecanii dispersible oil suspending agent as well as preparation method and application thereof
  • Verticillium lecanii dispersible oil suspending agent as well as preparation method and application thereof
  • Verticillium lecanii dispersible oil suspending agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This example provides the preparation of Verticillium lecanii spore powder in the dispersible oil suspension concentrate of Verticillium lecanii.

[0024] (1) Transfer the Verticillium lecanii species (L.lecanii patent preservation number CCTCC NO: M218303) stored in the PDA slant at 4°C to the PDA plate medium, and place in a constant temperature incubator at (25±1)°C Cultured in medium for 7 days, the spores were eluted with sterile water, and the configuration was 1×10 7 The cfu / mL spore suspension was stored at 4°C for later use.

[0025] (2) After soaking the rice in distilled water for 30 minutes, drain the water, pack in 1L tissue culture bottles, 300mL per bottle, sterilize at 121°C for 30 minutes, and inoculate the spore suspension at 1% after cooling to room temperature. Inoculate 1% of the inoculum spore suspension into 100 mL of medium and inoculate 1 mL of the spare spore suspension in (1). Shake well to disperse the rice in the tissue culture flask evenl...

Embodiment 2

[0028] This example provides a dispersible oil suspension of Verticillium lecanii with soybean oil as the dispersion medium.

[0029] Example 1 of source of Verticillium lecanii spore powder.

[0030] Components: 5 parts of Verticillium lecanii spore powder, 70 parts of soybean oil, 6 parts of vegetable oil emulsifier ZR-516, 4 parts of soybean oil emulsifier, 1 part of calcium lignosulfonate, 3 parts of organic bentonite, 1 part of sodium fluorescein share.

[0031] According to the above ratio requirements, weigh the quantitative soybean oil, vegetable oil emulsifier ZR-5, soybean oil emulsifier, calcium lignosulfonate, organic bentonite, sodium fluorescein, mix and shear for 30 minutes, and then add The spore powder of Verticillium lecanii is evenly cut, which is the dispersible oil suspension concentrate of Verticillium lecanii.

Embodiment 3

[0033] This example provides a dispersible oil suspension concentrate of Verticillium lecanii whose dispersion medium is methyl oleate.

[0034] Example 1 of source of Verticillium lecanii spore powder.

[0035] Components: 5 parts of Verticillium lecanii spore powder, 70 parts of methyl oleate, 12 parts of OP-101, 808 parts of Span, 1 part of Tween-801, 3 parts of organic bentonite, 1 part of sodium fluorescein.

[0036] According to the above ratio requirements, weigh quantitative methyl oleate, OP-10, Span 80, Tween-80, organobentonite, and fluorescein sodium, mix and cut for 30 minutes, and then add Verticillium cerevisiae in an ice bath. The fungus spore powder is cut evenly, which is the dispersible oil suspension concentrate of Verticillium lecanii.

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Abstract

The invention belongs to the technical field of pesticide preparations, and relates to a verticillium lecanii dispersible oil suspending agent and a preparation method thereof. The invention provides a Verticillium lecanii dispersible oil suspending agent which comprises Verticillium lecanii spore powder, a dispersion medium, an emulsifier, a dispersant, a viscosity modifier and an ultraviolet protective agent, the dispersion medium is one of soybean oil and methyl oleate; the emulsifier is one of a combination of a vegetable oil emulsifier ZR-5 and a soybean oil emulsifier and a combination of OP-10 and span 80; the dispersing agent is one of calcium lignosulphonate and Tween-80; the viscosity modifier is organic bentonite; the ultraviolet protective agent is fluorescein sodium. According to the verticillium lecanii dispersible oil suspending agent provided by the invention, a microorganism living body preparation is fully utilized, and a verticillium lecanii preparation which is good in dispersible oil suspending agent performance and insecticidal effect is obtained.

Description

technical field [0001] The invention belongs to the technical field of pesticide preparations and relates to a dispersible oil suspension for Verticillium lecanii. Background technique [0002] Pesticide formulations are developing towards multi-function, refinement, labor-saving and sustained release. Some new formulations that are safe, efficient, economical and environmentally compatible are constantly replacing those polluting emulsifiable concentrates, powders and other formulations. The suspending agent not only prevents the powder from polluting the environment, but also avoids the poisoning of benzene solvents in the emulsifiable concentrate to mammals. Dispersible oil suspension (OilDispersion, OD), as a new type of dosage form, is a liquid preparation in which the active ingredient is stably suspended in a water-immiscible liquid, and the oil suspension is used directly after dilution with water. Dispersible oil suspending agent has the advantages of small particl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01N25/04A01P7/04
CPCY02A50/30
Inventor 贾彦霞王芳柴军发洪波赵雪怡郭佳茹
Owner NINGXIA UNIVERSITY
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