Preparation method of glycosylated corn protein polypeptide carried xanthophyll nanoparticles

A corn protein and corn polypeptide technology, which is applied to the preparation methods of peptides, chemical instruments and methods, hydrolyzed protein components, etc. Problems such as unclear structure-activity relationship

Pending Publication Date: 2022-03-11
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, zein and its modified products have been studied as the carrier of active substances, but there are still deficiencies in the dissolution and release of lutein, absorption and utilization in the body, and nutritional safety. For example, Chinese patents CN103431156A and CN 106420666A disclose A zein nanoparticle and a preparation method thereof, and a preparation method of a glycosylated zein nano-carrier, wherein the zein and the glycosylated prolamin are respectively made into a nano-carrier through extrusion and jetting equipment Particles, but the prepared particles are relatively large, and they do not have good water solubility, release and absorption as nanocarriers
Another example is Chinese patent 103445282A, which discloses a preparation method of corn peptide

Method used

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  • Preparation method of glycosylated corn protein polypeptide carried xanthophyll nanoparticles
  • Preparation method of glycosylated corn protein polypeptide carried xanthophyll nanoparticles
  • Preparation method of glycosylated corn protein polypeptide carried xanthophyll nanoparticles

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specific Embodiment approach 1

[0026] Embodiment 1: A preparation method of lutein nanoparticles loaded with glycosylated zein polypeptide is realized by the following steps:

[0027] 1. A method for preparing lutein nanoparticles carried by glycosylated zein polypeptide, characterized in that: the method comprises the following steps:

[0028] (1) Enzymatic hydrolysis preparation of zein polypeptide

[0029] Disperse corn gluten powder into 10-20 times the volume of deionized water, add protease with 1.0%-3.0% protein powder mass, and enzymatically hydrolyze at 40-60°C for 60-120min. After the enzyme reaction, cook at 95°C for 5 minutes to inactivate the enzyme. After centrifugation, the supernatant is taken, and ultrafiltration membranes with different molecular weights are used to obtain corn peptide solutions with a certain molecular weight. After vacuum concentration and freeze-drying, corn protein hydrolyzed polypeptide powder is obtained.

[0030] (2) Preparation of glycosylated zein polypeptide

...

specific Embodiment approach 2

[0034]Specific embodiment two: The difference between this embodiment and specific embodiment one is that step (1) disperses zein powder with a purity greater than 95% into deionized water of 10 to 20 times the volume, and adds 1.8% protein powder mass ~2.7% neutral protease, enzymolysis at 50~55°C for 60~80min. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0035] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step (1), ultrafiltration membranes with molecular weight cut-offs of 10,000 Da and 5,000 Da are respectively used to obtain corn peptide solutions with molecular weights of 5,000 to 10,000 Da. Other steps are the same as in the first embodiment.

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Abstract

The problems that zein is easy to aggregate in water, poor in solubility, low in lutein loading efficiency, poor in dissolution controlled release effect and the like are solved. The preparation method of the glycosylated corn protein polypeptide carrying xanthophyll nanoparticles comprises the following steps: 1, carrying out enzyme hydrolysis on zein to obtain corn protein polypeptide with specific molecular weight; 2, preparing a corn protein hydrolyzed polypeptide subjected to glycosylation modification; and 3, preparing the high-load xanthophyll nanoparticles by taking the glycosylated corn peptide as a carrier. The invention has the advantages that: 1, the carrying and controlled release performance of the corn protein on the xanthophyll is improved; 2, the structural stability and digestion release property of the xanthophyll are improved; and thirdly, the nutrition safety is high, and the nutritional value of the fat-soluble lutein component can be improved. The preparation method of the modified corn polypeptide-xanthophyll nanoparticles has the advantages of high embedding efficiency on active ingredients such as fat-soluble xanthophyll, good dissolution dispersibility and strong nutrition functionality.

Description

Technical field: [0001] The invention relates to a preparation method of lutein nanoparticles carried by glycosylated zein polypeptide. Background technique: [0002] Most of zein is gliadin, which is mainly derived from corn yellow powder, a by-product of corn processing. It is a safe, non-toxic, low-cost, high biocompatibility and good degradability zein. However, due to the low solubility and poor absorption and utilization rate of corn protein, it is mainly used in feed to replace some feed protein at present, and the development and utilization rate is low. In recent years, studies have found that the non-polar amino acids of zein itself (accounting for about 50% of the total amino acid residues of zein, including leucine, alanine and proline), can self-assemble into spherical particles. , making it an ideal delivery vehicle for bioactive compounds, drugs, and other nutraceuticals and food ingredients. For example, the zein / soluble soybean polysaccharide compound is u...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K47/42A61K47/26A61K31/047A61K38/01A61K31/7008A61P27/02A61P27/10A61P39/06C12P21/06C07K1/34C07K1/107A23L33/105A23L29/00
CPCA61K9/5169A61K9/5123A61K9/5192A61K31/047A61K38/011A61K31/7008A61P27/02A61P27/10A61P39/06C12P21/06C07K1/34C07K1/1077A23L33/105A23L29/045A23V2002/00A61K2300/00A23V2250/211A23V2250/55
Inventor 焦岩常影崔璐麟孙晓宏宋志强韩赫刘志宇杨又彬
Owner QIQIHAR UNIVERSITY
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