Preparation method of glycosylated corn protein polypeptide carried xanthophyll nanoparticles

Abstract

The problems that zein is easy to aggregate in water, poor in solubility, low in lutein loading efficiency, poor in dissolution controlled release effect and the like are solved. The preparation method of the glycosylated corn protein polypeptide carrying xanthophyll nanoparticles comprises the following steps: 1, carrying out enzyme hydrolysis on zein to obtain corn protein polypeptide with specific molecular weight; 2, preparing a corn protein hydrolyzed polypeptide subjected to glycosylation modification; and 3, preparing the high-load xanthophyll nanoparticles by taking the glycosylated corn peptide as a carrier. The invention has the advantages that: 1, the carrying and controlled release performance of the corn protein on the xanthophyll is improved; 2, the structural stability and digestion release property of the xanthophyll are improved; and thirdly, the nutrition safety is high, and the nutritional value of the fat-soluble lutein component can be improved. The preparation method of the modified corn polypeptide-xanthophyll nanoparticles has the advantages of high embedding efficiency on active ingredients such as fat-soluble xanthophyll, good dissolution dispersibility and strong nutrition functionality.

Description

Technical field: [0001] The invention relates to a preparation method of lutein nanoparticles carried by glycosylated zein polypeptide. Background technique: [0002] Most of zein is gliadin, which is mainly derived from corn yellow powder, a by-product of corn processing. It is a safe, non-toxic, low-cost, high biocompatibility and good degradability zein. However, due to the low solubility and poor absorption and utilization rate of corn protein, it is mainly used in feed to replace some feed protein at present, and the development and utilization rate is low. In recent years, studies have found that the non-polar amino acids of zein itself (accounting for about 50% of the total amino acid residues of zein, including leucine, alanine and proline), can self-assemble into spherical particles. , making it an ideal delivery vehicle for bioactive compounds, drugs, and other nutraceuticals and food ingredients. For example, the zein / soluble soybean polysaccharide compound is u...

AI Technical Summary

Problems solved by technology

Currently, zein and its modified products have been studied as the carrier of active substances, but there are still deficiencies in the dissolution and release of lutein, absorption and utilization in the body, and nutritional safety. For example, Chinese patents CN103431156A and CN 106420666A disclose A zein nanoparticle and a preparation method thereof, and a preparation method of a glycosylated zein nano-carrier, wherein the zein and the glycosylated prolamin are respectively made into a nano-carrier through extrusion and jetting equipment Particles, but the prepared particles are relatively large, and they do not have good water solubility, release and absorption as nanocarriers

Another example is Chinese patent 103445282A, which discloses a preparation method of corn peptide glycosylation product nanoparticles embedding fat-soluble vitamins, and prepares fat-soluble nanoparticles such as corn peptide glycosylation product nanoparticles loaded with vitamins. High efficiency, small particle size, good transparency and stability of the solution, but the structure and properties of the glycosylation product used by the dry heat method are not easy to control, the loading affinity of the glycosylated protein is not significant, and the structure-activity relationship is not good. Clear, limited to embedding liquid oil-soluble vitamin D, E and other ingredients

Therefore, based on the above status quo, it can be seen that there is currently no method for preparing lutein nanoparticles with high efficiency by using glycosylated zein hydrolyzed polypeptides.

Images