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Nanometer delivery system and preparation method and application thereof

A delivery system and nano-technology, applied in the field of nano-delivery system and its preparation, can solve problems such as inability to combine gene therapy and unclear tumor antigens

Pending Publication Date: 2022-03-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although immunotherapy can overcome the limitations of gene therapy such as short treatment time, most tumor antigens are not clear, and gene therapy cannot be used in combination

Method used

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  • Nanometer delivery system and preparation method and application thereof
  • Nanometer delivery system and preparation method and application thereof
  • Nanometer delivery system and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] The preparation of embodiment 1 cationic liposome

[0085] (1) Take a certain amount of DOTAP and cholesterol, dissolve it in chloroform respectively, and prepare a storage solution with a concentration of 10 mg / mL;

[0086] (2) mixing DOTAP and cholesterol according to the molar ratio of 1:1;

[0087] (3) nitrogen volatilizes chloroform, and vacuumizes overnight to form a lipid film;

[0088] (4) Hydrate the lipid film with 50°C distilled water and homogenize it by ultrasonic;

[0089] (5) Squeeze the particle size with 200nm and 100nm filter membranes successively to obtain DOTAP / chol cationic liposomes.

Embodiment 2

[0090] The preparation of embodiment 2 guanidinium modified chitosan

[0091] (1) Prepare 1% hydrochloric acid solution, taking 30mL as an example, add 0.3mL concentrated hydrochloric acid to 29.7mL distilled water;

[0092] (2) Weigh 0.1g chitosan (Mr=30000~300000), add 10mL 1% hydrochloric acid solution to dissolve, and prepare 1% chitosan solution;

[0093] (3) In order to improve the reaction efficiency, the molar ratio of chitosan and dicyandiamide was adjusted to 1:2. The molecular weight of chitosan monomer is 161g / mol, so the molar weight of chitosan monomer is n (chitosan monomer)=0.1g / 161(g / mol)=0.000621nmol, then the mole of dicyandiamide The amount should be 0.001242 nmol, m(dicyandiamide) = 104.447 mg. Add dicyandiamide into 1% chitosan solution, stir to promote dissolution.

[0094] (4) After dicyandiamide and chitosan are completely dissolved, the reaction temperature is increased to 100°C, reacted for 3 hours, and cooled to room temperature to obtain the rea...

Embodiment 3

[0095] Purification and physical characterization of embodiment 3 guanidine-modified chitosan

[0096] (1) The reaction product obtained by cooling is placed in a 3500Da dialysis bag. In order to avoid the solubility of chitosan in an aqueous solution and reduce the precipitation, the dialysis is carried out in a weakly acidic environment (1% hydrochloric acid solution), and the dialysis is carried out for 3 hours to remove the non-participating Reactive dicyandiamide.

[0097] (2) Add 2 times the volume of absolute ethanol to the dialyzed solution to precipitate chitosan, and heat and volatilize the ethanol at 65°C to obtain the target reaction product.

[0098] (3) Part of the solid reaction product was taken out for physical characterization by infrared spectroscopy to identify its structure.

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Abstract

The invention discloses a nano delivery system and a preparation method and application thereof, the nano delivery system comprises a nano delivery carrier and a negative charge component, and the nano carrier comprises guanidyl modified chitosan, cationic liposome and an amphiphilic compound. The chitosan is subjected to guanidyl modification, so that the chitosan forms a polymer with more cations, and the entrapment amount and transfection efficiency of nucleic acid molecules can be improved. The nano-particles also have an immune activation effect, and the activation effect is better than that of traditional nano-particles prepared from protamine and cationic lipid. In the invention, the nanoparticles prepared on the basis of guanidyl modified chitosan can provide a new strategy for combined application of gene therapy and immunotherapy.

Description

technical field [0001] The invention belongs to the field of nano-biological materials, and in particular relates to a nano-delivery system and its preparation method and application. Background technique [0002] Due to its unique surface effects and quantum size effects, nanomaterials have shown outstanding advantages and broad application prospects in optics, electricity, and magnetism. With the development of nanoscience, the excellent functions exhibited by nanomaterials have given birth to their research and application in the field of biomedicine. At present, the research of nanobiomedicine mainly focuses on nanobiological effects and safety, nanotoxicology, biosensing, tissue engineering, medical imaging, drug delivery, disease (especially tumor) diagnosis and treatment, etc. Nanomaterials are increasingly showing unique advantages in dealing with cancer and other diseases with complex pathological environments and high heterogeneity. With the development and maturi...

Claims

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Application Information

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IPC IPC(8): A61K47/61A61K47/60A61K47/69A61K31/713A61K39/00A61K48/00A61P35/00A61P37/04C12N15/87
CPCA61K47/61A61K47/60A61K47/6935A61K47/6939A61K31/713A61K39/0011A61K48/0041A61K48/0008A61K48/005A61P35/00A61P37/04
Inventor 张元刘占燕
Owner SOUTH CHINA UNIV OF TECH
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