Preparation method and application of beer-based nitrogen-phosphorus co-doped biomass carbon dots

A biomass carbon, co-doping technology, applied in chemical instruments and methods, nanocarbon, fluorescence/phosphorescence, etc., can solve the problems of increased operation and cost, detection performance disadvantage, etc., to achieve low cost, rich surface functional groups, fluorescence The effect of high intensity and fluorescence quantum yield

Pending Publication Date: 2022-02-15
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Nitrogen, phosphorus, sulfur, silicon, boron, and manganese are common heteroatoms in carbon quantum dot doping; for the doping of heteroatoms in carbon quantum dots, the usual strategy is to add compounds containing this atom, such as nitrogen-doped Carbon quantum dots, whose raw materials are usually doped with urea, ammonia, and ethylenediamine; however, doping by adding exogenous compounds will undoubtedly increase the operation and cost of the experiment
[0008] The patent document (CN109880619A) discloses a method for detecting Fe(III) ions using nitrogen-doped lignocellulosic biomass-based carbon quantum dots, but the preparation of raw materials requires a series of cumbersome operations such as grinding, screening, and enzymatic hydrolysis; in addition , single nitrogen doping has certain disadvantages in detection performance compared with multi-element doping carbon dots

Method used

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  • Preparation method and application of beer-based nitrogen-phosphorus co-doped biomass carbon dots
  • Preparation method and application of beer-based nitrogen-phosphorus co-doped biomass carbon dots
  • Preparation method and application of beer-based nitrogen-phosphorus co-doped biomass carbon dots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] This example provides a method for preparing beer-based nitrogen and phosphorus co-doped biomass carbon dots, specifically:

[0048] Get 100 milliliters of commercially available beer, filter the beer with a microporous membrane of 0.22 microns, and obtain the beer filtrate;

[0049] Take 50 ml of beer filtrate and add it to a Teflon-lined stainless steel autoclave. After sealing the autoclave, put it into a blast drying oven for hydrothermal carbonization at 160°C for 6 hours to obtain a light yellow solution, and cool it down to room temperature naturally. ;

[0050] Pass the light yellow solution through a 0.22-micron microporous membrane and centrifuge; the centrifuged supernatant is dialyzed in pure water for 12 hours with a molecular cut-off of 500 Da to obtain a dialysate;

[0051] The obtained dialysate was freeze-dried at -80°C to obtain powdered beer-based nitrogen and phosphorus co-doped biomass carbon dots.

[0052] see figure 1 , is the rendering of the ...

Embodiment 2

[0061] This example discloses the use of beer-based nitrogen and phosphorus co-doped biomass carbon points to detect Fe 3+ The method is specifically: adding 1 milliliter of beer-based nitrogen and phosphorus co-doped biomass carbon dot dialysate prepared in Example 2, and 300 microliters of phosphate buffered saline solution (concentration is 10 millimoles, pH value is 7.4), mix well;

[0062] Prepare Fe at a concentration of 0 to 400 micromolar per liter 3+ Several parts of the standard solution, the above mixed buffer solution was added to different concentrations of Fe 3+ Remove from the standard solution, shake well, and let it stand for 5 minutes;

[0063] For each standard solution, the fluorescence spectrum of the solution was measured, and the excitation wavelength was 370 nm.

[0064] image 3 for different concentrations of Fe 3+ Quenching effect of standard solution on fluorescence intensity of beer-based nitrogen and phosphorus co-doped biomass carbon dots. ...

Embodiment 3

[0066] This embodiment discloses a method for detecting ascorbic acid with beer-based nitrogen and phosphorus co-doped biomass carbon points, specifically:

[0067] Add 1 milliliter of the beer-based nitrogen and phosphorus co-doped biomass carbon point dialysate prepared in Example 2, and 300 microliters of phosphate buffered saline solution (concentration is 10 mmol, pH value is 7.4) to the centrifuge tube, and mix well;

[0068] Prepare Fe at a concentration of 0 to 500 micromolar per liter 3+ Several parts of the standard solution, as a fluorescence quencher, the above mixed buffer solution was added to different concentrations of Fe 3+ Remove from the standard solution, shake well, and let it stand for 5 minutes;

[0069] Prepare several parts of ascorbic acid standard solution with a concentration of 0 to 300 micromoles per liter as a fluorescence recovery agent. After the fluorescence quenching of beer-based nitrogen and phosphorus co-doped biomass carbon dots reaches ...

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Abstract

The invention discloses a preparation method of beer-based nitrogen-phosphorus co-doped biomass carbon dots. The preparation method comprises the following steps: step 1, filtering beer to obtain beer filtrate, adding the beer filtrate into a reaction kettle, carrying out hydrothermal carbonization to obtain a faint yellow solution, and naturally cooling to room temperature; step 2, enabling the faint yellow solution cooled to the room temperature in the step 1 to pass through a membrane, centrifuging, and dialyzing the centrifuged supernate in pure water to obtain dialysate; and step 3, freeze-drying the dialysate obtained in the step 2 to obtain the powdery beer-based nitrogen and phosphorus co-doped biomass carbon dots. The invention also provides an application method thereof. The preparation method is simple, the biomass carbon dots can be obtained by performing one-step hydrothermal carbonization synthesis on the beer filtrate, the method is simple and feasible, the raw materials are cheap and easy to obtain, the cost is low, the yield is high, and the method is suitable for batch production.

Description

technical field [0001] The invention belongs to the fields of fluorescent carbon nanomaterials, biosensing technology and food analysis, and relates to a preparation method and application of beer-based nitrogen and phosphorus co-doped biomass carbon dots. Background technique [0002] Iron is an essential trace element for the human body. Iron deficiency in the human body can cause various diseases such as anemia and decreased resistance. Ascorbic acid is one of the most important water-soluble vitamins. As an important micronutrient and antioxidant, it plays an important role in the growth of the human body. , metabolism and development process play an important role, insufficient intake of ascorbic acid can lead to severe scurvy; dietary intake is an important way to maintain iron and ascorbic acid content in the body; therefore, the monitoring of dietary iron and ascorbic acid and evaluation are important. [0003] At present, many methods for the detection of iron ions...

Claims

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Application Information

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IPC IPC(8): C01B32/15G01N21/64C09K11/65
CPCC01B32/15G01N21/643C09K11/65G01N2021/6432
Inventor 方勇李彭王超孙昕炀丁俭樊凤娇
Owner NANJING UNIV OF FINANCE & ECONOMICS
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