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Culture method of asparagus and Italian wild interspecific hybridization F1-generation regenerated plants

A cultivation method and a technique for regenerating plants, which are applied in botany equipment and methods, plant regeneration, gardening methods, etc., can solve the problems of difficult rooting of regenerated plants of embryoid bodies, poor growth of embryoid bodies and regenerated plants, etc., and achieve Promote cell division and cell growth, high seedling rate, simple operation

Active Publication Date: 2021-12-31
VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the defects of the prior art, and provides a kind of asparagus cultivar and Italian wild interspecies hybrid F 1 The cultivation method of generation anther microspore culture induction to obtain embryoid regenerated plant, to solve the problem of hybridization between asparagus cultivars and Italian wild species under the cultivation conditions in the prior art. 1 The technical problems of embryoid bodies induced by the microspores of the anthers and poor growth of the regenerated plants
[0005] Another technical problem to be solved by the present invention is the hybridization between asparagus cultivars and Italian wild species under the cultivation conditions in the prior art. 1 Rooting Difficulty in Regenerated Plants Obtaining Embryoids Induced by Substituent Anther Microspores

Method used

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Experimental program
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Effect test

Embodiment 1

[0023] A kind of asparagus and Italian wild interspecific hybrid F 1 The culture method that generation anther microspore induces and obtains the embryoid body regeneration plant may further comprise the steps:

[0024] (1) Prepare solid culture medium and liquid culture medium and put into petri dish:

[0025] Solid medium: in MS medium, add 7g. L -1 Agar, 20g﹐ L -1 of sucrose and 10g. L -1 Glucose, pH5.8; liquid medium: in MS medium, add 40g. L -1 sucrose, 20g. L -1 Glucose, 0.5mg. L -1 The 6-benzylamino adenine 6-BA, 0.5mg. L -1 2,4-dichlorophenoxyacetic acid 2,4-D, 0.1mg﹒ L -1 Naphthaleneacetic acid NAA, 0.03mg. L -1 Glutathione L-GLU, 200mg. L -1 Glutamine GLU, pH 5.8;

[0026] The solid culture is sterilized at 121°C and 1.1MPa for 20 minutes, and the liquid medium is sterilized by filtration with a filter equipped with a 0.22 micron microporous membrane to ensure the sterility of the liquid medium; Inject 15mL of solid medium into the Petri dish bef...

Embodiment 2

[0032] A method for cultivating embryoid body regenerated plants induced by F1 generation anther microspores of asparagus and Italian wild interspecies hybridization, comprising the following steps:

[0033] (1) Prepare solid culture medium and liquid culture medium and put into petri dish:

[0034] Solid medium: in MS medium, add 7.5g. L -1 of agar and 20g. L -1 sucrose, 10g. L -1 Glucose, pH5.8; liquid medium: in MS medium, add 40g. L -1 sucrose, 20g. L -1 Glucose, 0.8mg. L -1 The 6-benzylamino adenine 6-BA, 1.0mg. L -1 2,4-dichlorophenoxyacetic acid 2,4-D, 0.5mg. L -1 Naphthaleneacetic acid NAA, 0.03mg. L -1 Glutathione L-GLU, 200mg. L -1 Glutamine GLU, pH 5.8;

[0035] The solid culture is sterilized at 121°C and 1.1MPa for 20 minutes, and the liquid medium is sterilized by filtration with a filter equipped with a 0.22 micron microporous membrane to ensure the sterility of the liquid medium; Inject 15mL of solid medium into the Petri dish before it soli...

Embodiment 3

[0041] A method for cultivating embryoid body regenerated plants induced by F1 generation anther microspores of asparagus and Italian wild interspecies hybridization, comprising the following steps:

[0042] (1) Prepare solid culture medium and liquid culture medium and put into petri dish:

[0043] Solid medium: in MS medium, add 8.0g. L -1 of agar and 20g. L -1 sucrose, 10g. L -1 Glucose, pH5.8; liquid medium: in MS medium, add 40g. L -1 sucrose, 20g. L -1 Glucose, 1.0mg﹑ L -1 The 6-benzylamino adenine 6-BA, 1.0mg. L -1 2,4-dichlorophenoxyacetic acid 2,4-D, 0.5mg. L -1 Naphthaleneacetic acid NAA, 0.03mg. L -1 Glutathione L-GLU, 200mg. L -1 Glutamine GLU, pH 5.8;

[0044] The solid culture is sterilized at 121°C and 1.1MPa for 20 minutes, and the liquid medium is sterilized by filtration with a filter equipped with a 0.22 micron microporous membrane to ensure the sterility of the liquid medium; Inject 15mL of solid medium into the Petri dish before it soli...

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Abstract

The invention discloses a culture method of asparagus and Italian wild interspecific hybridization F1-generation regenerated plants. The culture method comprises the following steps that suitable-period flower buds of an interspecific hybridization F1-generation are selected, sterilizing is carried out, anther is stripped, culturing is carried out, cotyledon type embryos are transferred into an embryo germination culture medium, illumination culture is carried out for 12-14 hours every day to develop and form robust cluster buds, and the robust cluster buds are transplanted into an embryo germination culture medium, so that the regenerated plants are obtained. According to the method, sucrose with relatively high concentration is adopted, indolebutyric acid, naphthylacetic acid and glutamine with proper concentration are emphatically added to jointly induce rooting and promote root development, the problem that asparagus and Italian wild interspecific hybridization F1-generation anther culture seedlings are difficult to root is effectively solved, and the rooting rate exceeds 80%. The prepared embryoid can grow normally, the seedling rate is high, operation is easy, time is saved, efficiency is high, and generally only 56-65 days are needed from starting of culture to obtaining of the embryoid.

Description

technical field [0001] The invention belongs to the field of plant cultivation, in particular to asparagus and Italian wild interspecies hybrid F 1 A culture method for obtaining embryoid body regenerated plants by inducing anther microspores. Background technique [0002] Asparagus (Asparagus officinalis L.), also known as Asparagus officinalis L., is a perennial herbaceous plant belonging to Asparagus family Asparagus. It is an important economic crop in the world. It is tender and delicious, rich in nutrition and unique in flavor. "King of Vegetables" has been popular in the international market for a long time and has high economic value. It is now the single vegetable variety with the largest processing and export trade volume in China. Because it is rich in various active ingredients such as saponins, flavonoids, plant polysaccharides and aspartic acid, it has good anti-tumor, anti-oxidation and blood pressure-lowering effects. Asparagus planting and processing not o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 汤泳萍周劲松张冰冰叶艳英尹玉玲罗绍春谢启鑫陈光宇
Owner VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI
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