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Method and device for rapidly detecting nickel ions

A nickel ion, fast technology, applied in the field of nickel ion detection, can solve the problems of inconvenient portability of the detection device, high cost and long time of nickel ion detection, and achieve the effects of sustainable development, fast detection speed and low cost

Active Publication Date: 2021-11-12
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, it is necessary to provide a method and device for rapid detection of nickel ions to solve the technical problems of high nickel ion detection cost, long time and inconvenient portability of the detection device in the prior art

Method used

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  • Method and device for rapidly detecting nickel ions
  • Method and device for rapidly detecting nickel ions
  • Method and device for rapidly detecting nickel ions

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Embodiment 1

[0042] The present embodiment provides a method for preparing a nickel ion detector and a standard colorimetric card, comprising the following steps:

[0043] (1) Preparation of color developing solution: 0.01 g of sodium hydroxide and 1 mmol of diacetyl oxime are added to 100 mL of water to dissolve, and the diacetyl oxime alkaline solution is prepared;

[0044](2) Preparation of bacterial cellulose membrane: prepare 500 mL of Acetobacter xylinum medium (containing 2.5wt% glucose, 0.5wt% yeast extract, 0.5wt% tryptone, 0.11wt% citric acid monohydrate, 0.11wt% dihydrogen phosphate Potassium, 0.5wt% disodium hydrogen phosphate dodecahydrate), adjust the pH of the medium to 6, inoculate 5mL of bacterial liquid (preservation number ATCC700178) under aseptic conditions, activate it in a constant temperature incubator at 30°C for 48h, and after activation, the bacteria The liquid was transferred to a 10×20×20cm plastic box, placed in a 30°C incubator for 10 days, cultured to obtain...

Embodiment 2

[0050] The present embodiment provides a method for preparing a nickel ion detector and a standard colorimetric card, comprising the following steps:

[0051] (1) Preparation of color developing solution: add 0.01 g of sodium hydroxide and 0.8 mmol / L of diacetyl oxime into 100 mL of water to dissolve, and prepare a diacetyl oxime alkaline solution;

[0052] (2) Preparation of bacterial cellulose membrane: prepare 500 mL of Acetobacter xylinum medium (containing 2.5wt% glucose, 0.5wt% yeast extract, 0.5wt% tryptone, 0.11wt% citric acid monohydrate, 0.11wt% dihydrogen phosphate Potassium, 0.5wt% disodium hydrogen phosphate dodecahydrate), adjust the pH of the medium to 6, inoculate 5ml of bacterial liquid (preservation number ATCC700178) under sterile conditions, activate it in a constant temperature incubator at 30 ° C for 48h, after activation, the bacteria The solution was transferred to a 10×20×20cm plastic box, placed in a 30°C incubator for 14 days, cultured to obtain a fi...

Embodiment 3

[0058] The present embodiment provides a method for preparing a nickel ion detector and a standard colorimetric card, comprising the following steps:

[0059] (1) Preparation of color-developing solution: 0.01g of sodium hydroxide and 1.2mmol of dimethylglyoxime are added to 100mL of water to dissolve, to prepare a dimethylglyoxime alkaline solution;

[0060] (2) Preparation of bacterial cellulose membrane: prepare 500 mL of Acetobacter xylinum medium (containing 2.5wt% glucose, 0.5wt% yeast extract, 0.5wt% tryptone, 0.11wt% citric acid monohydrate, 0.11wt% dihydrogen phosphate Potassium, 0.5wt% disodium hydrogen phosphate dodecahydrate), adjust the pH of the medium to 6, inoculate 5mL of bacterial liquid (preservation number ATCC700178) under aseptic conditions, activate it in a constant temperature incubator at 30°C for 48h, and after activation, the bacteria The solution was transferred to a 10×20×20cm plastic box, placed in a 30°C incubator for 12 days, cultured to obtain ...

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Abstract

The invention discloses a method and a device for rapidly detecting nickel ions. The method for rapidly detecting nickel ions comprises the following steps: preparing a developing solution, preparing a carboxylated bacterial cellulose membrane, preparing a nickel ion detector, preparing a standard colorimetric card, and detecting a sample to be detected. According to the nickel ion detector, the concentration of nickel ions can be rapidly, simply and conveniently detected, the detection lower limit can be accurate to the ppm level, the whole detection process only needs 1-3 minutes, the detection result can be obtained on site, and the detection efficiency is improved; the use process is simple, professional personnel are not needed, and daily detection requirements of common people can be met; and the nickel ion detector is small in size, easy to carry, low in cost and high in detection speed, only the detector and a standard colorimetric card are needed in the use process, other solutions or large devices are not needed, detection is extremely convenient, environmental pollution is avoided, energy is saved, environment friendliness is achieved, use is convenient, and sustainable development of technical economy is facilitated.

Description

technical field [0001] The invention relates to the technical field of nickel ion detection, in particular to a method and a device for rapid detection of nickel ions. Background technique [0002] Nickel is one of the essential elements of the human body. Under normal circumstances, the adult body contains about 10mg of nickel. Nickel is also one of the most easily sensitized elements in daily life. People who are sensitized by exposure to nickel will experience symptoms such as inflammation, itching, papulo-vesicular dermatitis and eczema, and the allergic symptoms can last indefinitely. When the human body ingests excessive nickel, vomiting, diarrhea, gastroenteritis, etc. will occur, and in severe cases, it will lead to edema, water discharge and degeneration of organs. Nickel in cigarettes can irritate and damage the lungs. Therefore, controlling and reducing nickel intake is indispensable. [0003] Nickel has good plasticity, corrosion resistance and magnetic proper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 张甜蒋向阳赵子懿廖雨潇皮埃尔
Owner WUHAN UNIV OF TECH
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