Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection method of endogenous glucocorticoid and application thereof

A glucocorticoid and detection method technology, applied in the field of analytical chemistry, can solve problems such as high quantitative limit, inability to apply to people of different races and different constitutions, poor sensitivity of glucocorticoid, etc., achieve high degree of automation, good selectivity, and improve repeatability Effects on sex and stability

Pending Publication Date: 2021-06-11
SHANGHAI UNIV OF SPORT
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing detection of glucocorticoids has poor sensitivity and high limit of quantification, and cannot be applied to the detection of people of different races, ages, and constitutions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of endogenous glucocorticoid and application thereof
  • Detection method of endogenous glucocorticoid and application thereof
  • Detection method of endogenous glucocorticoid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1 of the present invention provides a kind of detection method of endogenous glucocorticoid, specifically as follows:

[0088] (1) Extraction of glucocorticoids:

[0089] Take 200uL of urine, add 20μL of internal standard, 100μL of phosphate buffered saline (PBS) and 10μL of enzyme, mix well, and enzymolyze at 55°C in a water bath for 2 hours; Ether, shake (2500rpm speed) for 3min; then centrifuge for 15min, centrifuge temperature 4°C, speed 13000rpm, take 600μL of supernatant, N 2 Blow dry, add 50 μ L of extract (V water: V acetonitrile = 4:1) to redissolve, obtain a urine test sample, to be tested; the phosphate buffer is a mixed solution of sodium dihydrogen phosphate and disodium hydrogen phosphate, The pH is 6.75; the enzyme is glucuronidase; the lye is sodium bicarbonate and potassium carbonate, and the pH is 9.5.

[0090] (2) Liquid chromatography detection:

[0091] The urine test sample that step (1) obtains is carried out liquid chromatography de...

Embodiment 2

[0119] Example 2 of the present invention provides a detection method for endogenous glucocorticoids, the specific implementation of which is the same as that of Example 1, except that the pH of the phosphate buffer is 7.5.

[0120] Cortisone standard curve drawing, limit of detection, limit of quantitation test method are the same as embodiment 1, obtain R 2 is 0.98725, the LOD is 0.1 ng / mL, and the LOQ is 1.5 ng / mL.

Embodiment 3

[0122] Example 3 of the present invention provides a detection method for endogenous glucocorticoids, the specific implementation of which is the same as that of Example 1, except that the amount of methyl tert-butyl ether added is 0.

[0123] Cortisone standard curve drawing, limit of detection, limit of quantitation test method are the same as embodiment 1, obtain R 2 is 0.81275, the LOD is 1 ng / mL, and the LOQ is 5 ng / mL.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of analytical chemistry, and particularly relates to a detection method of endogenous glucocorticoid and application thereof. The detection method of the endogenous glucocorticoids comprises the following steps: optimizing extraction conditions of a plurality of endogenous glucocorticoids in a urine sample, establishing a mass spectrometric detection process and analyzing a crowd value of an exercise training sample. According to the detection method of the endogenous glucocorticoid, the obtained LOD is 0.1 ng / mL, the obtained LOQ is 1 ng / mL, the extraction recovery rate is larger than 87%, and the matrix effect, the precision and the accuracy are all within + / -15%. The method provided by the invention can meet daily detection requirements, can realize high-throughput sample pretreatment during actual sample detection, saves time cost, reduces manual operation errors, and improves reproducibility and stability.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and more specifically, the invention relates to a detection method of endogenous glucocorticoids and an application thereof. Background technique [0002] Glucocorticoid (GC) is an extremely important class of regulatory molecules in the body. It plays an important role in regulating the development, growth, metabolism and immune function of the body. It is the most important regulatory hormone for the body's stress response. The most widely used and effective anti-inflammatory and immunosuppressant. In urgent or critical situations, glucocorticoids are often the first choice. Common clinical glucocorticoid drugs include prednisone, methylprednisone, betamethasone, beclomethasone dipropionate, prednisolone, hydrocortisone, dexamethasone, etc., which have anti-toxic, anti-allergic, Anti-shock, non-specific immune suppression and antipyretic effects can prevent and prevent the occurr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N33/74
CPCG01N30/02G01N33/74
Inventor 刘冰龙紫阳卓悦徐昕陈佩杰候飞
Owner SHANGHAI UNIV OF SPORT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com