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Traditional Chinese medicine composition for resisting HPV infection and preparation method thereof

A technology of composition and traditional Chinese medicine, which is applied in the direction of drug combination, antineoplastic drugs, drug delivery, etc., can solve the problems of high recurrence rate, low efficiency, easy recurrence, and difficult evaluation, so as to inhibit cervical precancerous lesions, ensure reproductive function, The effect of avoiding the pain of surgery

Active Publication Date: 2021-10-22
苏州龙达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing types of anti-HPV products mainly include: α-interferon immunomodulatory type, which does not directly affect the virus and enhances the immune response, with unsatisfactory curative effect and high recurrence rate; corrosive chemical topical drugs, which are ineffective and easy to relapse, and are effective against high-risk HPV Viruses are ineffective; biological protein dressing type, biological protein charge non-specific combination, mainly plays a physical isolation role, has limited direct effect on viruses, and the curative effect varies too much from individual to individual, making it difficult to evaluate; most products are ineffective against persistent infection, and most of them have no clinical symptoms HPV infection is transient, and the infected person will heal itself without using the product, so most of the existing products have fished in troubled waters and are ineffective for persistent infection

Method used

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  • Traditional Chinese medicine composition for resisting HPV infection and preparation method thereof
  • Traditional Chinese medicine composition for resisting HPV infection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation of Chinese medicine composition of the present invention

[0029] S1. Take 5 parts of black nightshade, 12 parts of sophora flavescens, 8.5 parts of Andrographis paniculata, 2 parts of rhubarb and 8.5 parts of indigo naturalis, wash and dry at 40°C for 15 hours, and then pulverize at 10000r / min for 60s to obtain a coarse powder.

[0030] S2. The coarse powder obtained in step S1 is crushed with a vibrating grinding type broken wall superfine pulverizer under the condition of 960r / min for 40min, the temperature is -15°C, and pulverized until the particle size is below 25 microns to obtain superfine broken wall powder ; Ultrafine broken wall powder is sterilized by cobalt 60 rays.

[0031] S3. First add 1 part of carbomer, 1-2 parts of low-molecular-weight sodium hyaluronate, and 0.1 part of sodium ethylparaben, and add distilled water to 85 parts, and fully swell to prepare a gel matrix. Add the gel matrix to 15 parts of the superfine wall-...

Embodiment 2

[0033] Embodiment 2: Pharmacological efficacy experiment

[0034] The antiviral activity of the gel paste prepared in Example 1 was tested, and the activity against human papillomavirus HPV16 was mainly detected. The detection basis is "Disinfection Technical Specifications" (2002 edition), with Vero-E6 cells as the target cells, the gel paste prepared in Example 1 as the test group, the S3 step in Example 1, without adding 15 parts of ultramicro The gel matrix prepared by broken wall powder was used as the control group. Three parallel experiments were set up in the experimental group and the control group respectively. Human papillomavirus HPV16 was added to the normal growth medium of the target cell Vero-E6 cells for co-incubation, and then the condensate was added. Glue paste or gel base, observe the inhibitory effect of gel paste or gel base on human papillomavirus HPV16, the test results are shown in the table below. The results show that the gel ointment prepared by the...

Embodiment 3

[0037] Embodiment 3: toxicology experiment

[0038] This embodiment illustrates the safety of the gel paste based on in vitro cytotoxicity:

[0039] In this embodiment, Vero-E6 cells are used as target cells, and they are co-incubated with media containing different concentrations of gel paste. The results show that different drug doses have different toxicity to Vero-E6 cells, and the cell survival rate is proportional to the concentration of gel paste. Inversely, the results are shown in the table below:

[0040] Table 2

[0041]

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Abstract

The invention discloses a traditional Chinese medicine composition for resisting HPV infection and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw material medicines: black nightshade, radix sophorae flavescentis, common andrographis herb, rheum officinale and indigo naturalis. The effective components in the traditional Chinese medicine composition make full contact with vagina and cervical mucosa by means of high permeability and directly reach basal layer cells, viruses can be killed more thoroughly and deeply, the curative effect on HPV infection is remarkable, the negative conversion rate reaches 88% or above, precancerous lesions of cervical cancer are effectively inhibited, and sequelae of lesions below the CIN II level is achieved. The effective rate of treating chronic inflammation such as cervical erosion and vaginitis reaches 98% or above.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition for resisting HPV infection and a preparation method thereof, belonging to the technical field of traditional Chinese medicines. Background technique [0002] Cervical cancer is one of the three major malignant tumors of the female reproductive system, and it is the fourth most morbid and fatal tumor among women in the world. The number of new cases in China is 130,000 per year. The pathogenesis of cervical cancer is closely related to the persistent infection of high-risk human papillomavirus (HR-HPV). HPV can be detected in all invasive cervical cancers and 95% of cervical intraepithelial neoplasia and carcinoma in situ. High-risk HPVs are mainly HPV16 and HPV18, and the main tumorigenic proteins E6 and E7 expressed by HPV play a key role in the process of developing cervical cancer from HPV infection. 70-80% of women have been infected with HPV in their lifetime, and 10% of them ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/81A61K9/06A61K47/32A61K47/36A61P15/00A61P29/00A61P31/20A61P35/00
CPCA61K36/81A61K36/489A61K36/19A61K36/708A61K36/704A61K36/315A61K36/195A61K9/06A61K47/32A61K47/36A61K9/0034A61P31/20A61P35/00A61P15/00A61P29/00A61K2236/15A61K2300/00
Inventor 李丽娅尹斯佳李丽琦
Owner 苏州龙达生物科技有限公司
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