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Detection method of common goldenrop particles

A technology of Xiaobupleurum granule and determination method, which is applied in the field of detection of Xiaochaihu granule, can solve the problems of cumbersome and complicated detection methods, many mobile phase channels, and many organic reagents, and achieve improved quality control level, good universality, and repeatability. sex high effect

Active Publication Date: 2021-06-08
GUANGZHOU BAIYUSN GUANGHUA PHARMA
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Problems solved by technology

There are obvious deficiencies in the above method: (1) the fingerprint and content determination are detected by different methods; (2) the fingerprint is measured by a nonlinear gradient, which is not easy to reproduce, and the reproducibility is relatively poor; Three kinds of solutions of acetonitrile and 0.2% phosphoric acid are used as the mobile phase, and there are many channels for the mobile phase, which consumes a lot of organic reagents, making the detection method cumbersome and complicated; (4) The detection time is 105 minutes, which takes a long time and is not efficient

Method used

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  • Detection method of common goldenrop particles
  • Detection method of common goldenrop particles
  • Detection method of common goldenrop particles

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preparation example Construction

[0061] Preparation of mixed reference solution: take baicalin, baicalein, wogonin, wogonin, ammonium glycyrrhizinate, saikosaponin b 2 , saikosaponin b 1 Reference substance, diluted with solvent, as a mixed reference substance solution;

[0062] Preparation of the reference medicinal material solution: take the reference medicinal materials of Bupleurum, Radix Scutellariae, Radix Glycyrrhizae, Codonopsis Radix, and Jujube, add extraction solvent to dissolve, filter, and take the subsequent filtrate as the reference medicinal material solution;

[0063] The preparation of need testing solution: get Xiao Bupleurum granule, add extracting solvent to dissolve, filter, get continued filtrate and be need testing solution;

[0064] The mixed reference substance solution, the reference medicinal material solution and the test solution are respectively injected into a high-performance liquid chromatograph for measurement to obtain fingerprints;

[0065] The mobile phase adopted by t...

Embodiment 1

[0071] Instruments and reagents

[0072] Instrument: ultra-fast high-performance liquid mass spectrometer (LC-20AD-XR binary pump, SIL-20AD-XR autosampler, CTO-20A column thermostat, SPD-M10A-PDA detector) from Japan SHIMADZU company; Agilent1260 high-efficiency Liquid chromatography (Germany Agilent, G1311B single quaternary pump, G1329B autosampler, G1316A column thermostat, G1315D detector, OPENLABCDS ChemStation Edition data processing software); Dionex Ultimate 3000DGLC high performance liquid chromatography (DGP-3600SD Double ternary pump, SRD-3600 degasser, WPS-3000SL autosampler, TCC3000-RS column thermostat, DAD detector); Chromatographic column: Phecda-C18 (4.6×250mm, 5μm).

[0073] Test drug: 10 batches of finished products were provided by Guangzhou Baiyunshan Guanghua Pharmaceutical Co., Ltd. (K00226, K00227, K00228, K00229, K00230, K00231, K00232, K00233, K00234, K00235). The acetonitrile and phosphoric acid reagents used in the liquid chromatography in the expe...

Embodiment 2

[0120] Example 2 Determination of content of index components in Xiaobupleurum granules

[0121] 1. Preparation of the test solution: Take the Xiaobupleurum granules of this product, grind them finely, take about 1.0g, weigh them accurately, put them in a stoppered Erlenmeyer flask, add 10ml of 70% ethanol precisely, seal the plug, weigh it, Sonicate for 30 minutes, let cool, weigh again, make up the lost weight with 70% ethanol, shake well, pass through a 0.45 μm filter membrane, and take the subsequent filtrate to obtain the final product.

[0122] 2. Preparation of mixed reference substance solution: Weigh appropriate amounts of baicalin and ammonium glycyrrhizinate reference substances respectively, place them in the same volumetric flask, add 70% ethanol to make a mixed reference substance containing 0.26 mg of baicalin and 40 μg of glycyrrhizic acid per ml solution. (Glycyrrhizic acid=ammonium glycyrrhizinate / 1.0207).

[0123] 3. High performance liquid chromatography ...

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Abstract

The invention relates to a detection method of common goldenrop particles. The detection method of the common goldenrop particles comprises the following steps: preparation of a mixed reference substance solution: taking baicalin, baicalein, wogonin, wogonoside, ammonium glycyrrhizinate, saikoside b2 and saikoside b1 reference substances, adding a solvent for dilution to as the mixed reference substance solution and preparing a reference medicinal material solution; preparation of a test solution: taking common goldenrop particles, adding an extraction solvent for dissolving, filtering, and taking a subsequent filtrate as the test solution; and respectively injecting the mixed reference substance solution, the reference medicinal material solution and the test solution into a high performance liquid chromatograph for determination. According to the method, 15 components of 5 medicinal materials of the common goldenrop particles and 2 components of 2 medicinal materials can be subjected to qualitative treatment and quantitative treatment at the same time, the operation is simple and convenient, the repeatability is high, and the chromatographic peak pattern is good.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicines, in particular to a detection method for Xiaobupleurum granules. Background technique [0002] The Xiaobupleurum granule of the present invention is a traditional Chinese medicine granule made from the traditional Chinese medicine Bupleurum Radix, Scutellaria Baicalensis, Licorice Root, Codonopsis Codonopsis, Ginger Pinellia, and Jujube. Evil criminal Shaoyang syndrome. Xiaochaihu granules have made important contributions to the prevention and treatment of SARS, influenza A, dengue fever, and new coronary pneumonia in recent years, and have been recognized by many doctors and patients. They have a good reputation and broad market prospects. [0003] In the current Pharmacopoeia standards, the thin-layer identification of Xiaochaihu granules is used to characterize baicalin, licorice and Bupleuri, respectively, and the content determination index is baicalin, which ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/8686G01N2030/324
Inventor 郑如文刘宏周毅红司徒文辉戴艳萍江志强张俊华胡卫林杜海泳
Owner GUANGZHOU BAIYUSN GUANGHUA PHARMA
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