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rH-PLGA/PEI microsphere and dopamine modified small-caliber intravascular stent material and preparation method thereof

A vascular stent, dopamine technology, applied in pharmaceutical formulations, microcapsules, transportation and packaging, etc., can solve problems such as inability to provide anticoagulant properties, and achieve the goal of promoting vascular endothelial cell adhesion, promoting anticoagulant properties, and accelerating speed and quality. Effect

Inactive Publication Date: 2021-06-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this preparation method protects the functional group in the binding region of thrombin from being reacted to affect the structural domain binding to thrombin, it can also make hirudin bind to polyethylene glycol diamine cationization with a strong ionic bond. On the silk fibroin, the obtained anticoagulant material has the function of significantly inhibiting the activity of thrombin, but it cannot provide sustained anticoagulation

Method used

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  • rH-PLGA/PEI microsphere and dopamine modified small-caliber intravascular stent material and preparation method thereof
  • rH-PLGA/PEI microsphere and dopamine modified small-caliber intravascular stent material and preparation method thereof
  • rH-PLGA/PEI microsphere and dopamine modified small-caliber intravascular stent material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Step 1: Preparation of PCL nanofiber scaffold

[0046] Add polycaprolactone to hexafluoroisopropanol, PCL / hexafluoroisopropanol=1.5g / 15ml, stir for 12 hours to make a spinning solution, and use electrospinning technology to prepare a PCL nanofiber support; wherein, the spinneret The distance from the receiving iron plate is adjusted to 18cm; the flow rate of the spinning solution in the syringe pump is 2ml / h, and the liquid volume is adjusted to 8ml; the rotation speed of the rotating motor is adjusted to 100 rpm; It is 11kv; turn on the power of the rotating motor, turn on the switch of the injection pump, and the nanofiber bundle will be ejected from the spinning needle. After the spinning is stable, the continuous spinning time will be 240 minutes. After the spinning is completed, turn off the switch of the syringe pump and the motor in turn. Power supply, obtain PCL nanofiber support;

[0047] Step 2: Preparation of rH-PLGA-PEI microspheres

[0048] Water-in-oil-i...

Embodiment 2

[0054] Step 1: Preparation of PCL nanofiber scaffold

[0055] Add polycaprolactone in hexafluoroisopropanol, PCL / hexafluoroisopropanol=2g / 15ml, stir for 24h to make spinning solution, and use electrospinning technology to prepare PCL nanofiber support; wherein, the spinneret and The distance to accept the iron plate is adjusted to 18cm; the flow rate of the spinning liquid in the syringe pump is 2ml / h, and the liquid volume is adjusted to 8ml; the rotating speed of the rotating motor is adjusted to 100 rpm. After the power is turned on, the voltage of the high-voltage direct current generator is adjusted to 11kv; after turning on the power supply of the rotating motor and turning on the switch of the injection pump, the nanofiber bundles are ejected from the spinning needle. After the spinning is stable, the continuous spinning time is 240 minutes. After the filament is finished, turn off the syringe pump switch and the motor power supply in turn to obtain the PCL nanofiber sc...

Embodiment 3

[0064] Test of anticoagulant performance of rH-PLGA / PEI microspheres and dopamine-modified small-caliber vascular stent materials. Film samples (1.0×1.0cm 2 ) was placed in a cuvette, and 0.1 mL of plasma (PPP) was incubated at 37°C for 30 minutes. Then, the plasma coagulation time (ie APTT value) was recorded by an automatic coagulation analyzer, and the test was repeated three times to obtain the average value. No sample film was added to the blank control group, and other operating conditions and operating methods were exactly the same as those of the experimental group. APTT results such as Figure 4 As shown, the results show that the APTT of rH-PLGA / PEI microspheres and dopamine-modified small-caliber vascular scaffolds is 8.8s longer than that of blank PCL, indicating that the loading of rH-PLGA / PEI microspheres significantly improves the anticoagulant properties of PCL . Figure 4 PPP in the abscissa means blank control group, PCL means PCL nanofiber scaffold, PDA-...

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Abstract

The invention discloses a rH-PLGA / PEI microspheres and dopamine modified small-caliber intravascular stent material and a preparation method thereof. The method comprises the following steps: preparing a PCL nanofiber scaffold as a substrate by using an electrostatic spinning technology, depositing a layer of dopamine on the surface of the PCL nanofiber scaffold, and loading PLGA / PEI microspheres wrapped with hirudin on PCL nanofibers by using the adhesion of the dopamine to obtain the small-caliber intravascular stent material. According to the method, hirudin, PLGA / PEI microspheres and dopamine are combined, the obtained stent can prevent thrombus and promote rapid endothelialization and revascularization for a long time, the process is simple and feasible, and repeatability is good. The small-caliber intravascular stent has good biocompatibility, and the vascular tissue repair speed and quality are accelerated by utilizing the promotion effect and anticoagulant effect of hirudin on endothelial cell adhesion and proliferation.

Description

technical field [0001] The invention belongs to the field of degradable vascular stents, and in particular relates to a small-diameter vascular stent material modified by rH-PLGA / PEI microspheres and dopamine and a preparation method thereof. Background technique [0002] With the improvement of living standards, the aging of the social population has become a trend, and the main culprit of the death of the aging population is cardiovascular disease. In addition to drug therapy, stent interventional surgery and vascular grafting have become the main means of atherosclerosis treatment. [0003] At present, the replacement of large blood vessels has achieved good clinical results. However, the transplantation of small-caliber blood vessels still has a low long-term patency rate and faces problems such as secondary surgery. Therefore, preparing synthetic materials with good biocompatibility, meeting the property requirements of blood vessels, preventing thrombus formation, and...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/54A61L27/58A61L33/00A61L33/12
CPCA61L27/18A61L27/54A61L27/58A61L33/0041A61L33/128A61L2400/12A61L2300/622A61L2300/602A61L2300/42C08L67/04C08L71/02
Inventor 杜昶戴欣郑志雯李雪杨
Owner SOUTH CHINA UNIV OF TECH
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